T hestudyofinteractionbetweenRab8andERMfamily i ntheocularciliarybody

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592253
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: 独立行政法人国立病院機構東京医療センター(臨床研究センター) (2011); 独立行政法人国立病院機構(東京医療センター臨床研究センター) (2009-2010)
• Principal Investigator: KIMURA Itaru 独立行政法人国立病院機構東京医療センター(臨床研究センター), 分子細胞生物学研究部, 外部研究員 (60296663)
• Co-Investigator(Renkei-kenkyūsha): IWATA Takeshi 東京医療センター臨床研究センター, 分子細胞生物学研究部, 部長 (90374157)
• Project Period (FY): 2009 – 2011
• Keywords: 緑内障 / 眼圧 / 房水 / 毛様体 / Rab8 / ERM family

Research Abstract

Using wild type mice and healthy cynomolgus monkeys, we prepared ocular sections, performed immuno-staining and found the co-existence of Rab8 and ERM family in the ocular ciliary body. However, significant results from immuno-staining of ocular sections in Rab8-deficient mice were not found. We performed western blot analysis of Rab8 using cultured ocular ciliary epithelium cells of cynomolgus monkeys, and found that the Rab8 reduced concentration-dependently when dexamethasone was added to culture medium, whereas obvious changes were not found when other drugs were added.

Research Products

• [Journal Article] Mutant WDR36 directly affects axon growth of retinal ganglion cells leading to progressive retinal degeneration in mice. (2010)
  • Author(s): Chi ZL, Yasumoto F, Sergeev Y, Minami M, Obazawa M, Kimura I, Takada Y, Iwata T
  • Journal Title: Hum Mol Genet Volume: 19 Pages: 3806-3815
  • DOI: DOI:10.1093/hmg/ddq299
  • Peer Reviewed
• [Journal Article] Elevated serum immuno globulin G titers against Chlamydia pneumoniae in primary open angle glaucoma patients without systemic disease (2010)
  • Author(s): Yuki K, Kimura I, Shiba D, Imamura Y, Tsubota K.
  • Journal Title: J Glaucoma Volume: 19 Pages: 535-539
  • DOI: DOI:10.1097/IJG.0b013e3181ca7868
  • Peer Reviewed
• [Presentation] Normotension Glaucoma (2012)
  • Author(s): Kimura I
  • Organizer: Scientific Meeting West Java Region IOA
  • Place of Presentation: Grand Royal Panghegar Hotel, Bandung, Indonesia
  • Year and Date: 2012-03-31
• [Presentation] ほか、ラタノプロスト/チモロール配合点眼への切替えによる眼圧下降効果の検討(中間報告) (2011)
  • Author(s): 木村至
  • Organizer: 第22回日本緑内障学会
  • Place of Presentation: 秋田ビューホテル
  • Year and Date: 2011-09-24
• [Presentation] Analysis of colocalization of Rab8 and ERM family in the ocular ciliary body (2010)
  • Author(s): Kimura I, Iwata T, et al
  • Organizer: ARVO 2010 Annual meeting
  • Place of Presentation: Fort Lauderdale, USA
  • Year and Date: 2010-05-04
• [Presentation] 毛様体におけるRab8 とERM family の局在についての検討 (2009)
  • Author(s): 木村至、岩田岳
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2009-04-16
• [Presentation] 緑内障遺伝子WDR36トランスジェニックマウスの解析 (2009)
  • Author(s): 池在龍、木村至、岩田岳
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2009-04-16
• [Book] 専門医のための眼科診療クオリファイ3緑内障診療ガイド、診断の基本問診と診診 (2010)
  • Author(s): 木村至
  • Total Pages: 08-13
  • Publisher: 中山書店