Morphological and functional changes in visual cortex after retinal damage and its pharmacological approach

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592262
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Gifu Pharmaceutical University
• Principal Investigator: HARA Hideaki 岐阜薬科大学, 薬学部, 教授 (20381717)
• Co-Investigator(Renkei-kenkyūsha): SHIMAZAWA Masamitsu 岐阜薬科大学, 薬学部, 准教授 (80381721) ; ARAIE Makoto 東京大学, 医学部付属病院眼科, 教授 (00092122) ; WATANABE Yasuyoshi 大阪市立大学, 大学院・医学研究科・システム神経科学, 教授 (40144399)
• Project Period (FY): 2009 – 2011
• Keywords: 眼科学

Research Abstract

We examined lateral geniculate nucleus(LGN) degeneration secondary to experimental hypertensive glaucoma as an indicator for glaucoma in ocular hypertensive monkeys using positron emission tomography(PET), and validated glial activation by morphological and immunohistochemical examinations. Furthermore, we investigated whether endoplasmic reticulum(ER) stress was involved in the pathophysiological mechanisms underlying neuronal death of the LGN after intraocular pressure(IOP) elevation. In the present study, we showed that ER stress may play a pivotal role in neuronal death of the LGN after IOP elevation, that glial activation occurs in the LGN, and that the LGN degeneration could be detected by a PET imaging after unilateral ocular hypertension. Therefore, activated glial markers such as PBR in the LGN may be useful in noninvasive molecular imaging for diagnosis of glaucoma.

Research Products

• [Journal Article] An alteration in the lateral geniculate nucleus of experimental glaucoma monkeys : In vivo positron emission tomography imaging of glial activation (2012)
  • Author(s): Shimazawa M., Ito Y., Inokuchi Y., Yamanaka H., Nakanishi T., Hayashi T., Ji B., Higuchi M., Suhara T., Imamura K., Araie M., Watanabe Y., Onoe H. and Hara H.
  • Journal Title: PLoS ONE Volume: 7 Pages: e30526
• [Journal Article] 眼圧非依存障害因子への挑戦-ネズミ・サル・そしてヒトへ- (2012)
  • Author(s): 新家眞、北澤克明、山本哲也、澤田明、岩瀬愛子、山本精一郎、吉村健一、原英彰、嶋澤雅光、伊藤保志、山嶋哲盛、今村和幸、笹岡正顕、太田貴史、水野憲、川北耕二、大橋靖雄、白土城照、山崎芳夫、松本長太、玉置泰裕、相原一、富所敦男、永原幸、山上淳吉、古関信之、石井清、高山淳、長谷川智之、間山千尋、内田彩子、山田秀之、大橋正明、Yi-Ning Chen、佐伯忠賜朗、重枝崇史、村田博、Kelvin Lee、鶴我英和、Mao Nakayama、山岸麗子、緑内障
  • Journal Title: 日本眼科学会雑誌 Volume: 115 Pages: 213-237
• [Journal Article] Involvement of endoplasmic reticulum stress on neuronal cell death in the lateral geniculate nucleus in the monkey glaucoma model (2011)
  • Author(s): Ito Y., Shimazawa M., Inokuchi Y., Yamanaka H., Tsuruma K., Imamura K., Onoe H., Watanabe Y., Aihara M., Araie M. and Hara H.
  • Journal Title: European Journal of Neuroscience Volume: 33 Pages: 843-855
• [Journal Article] PETを用いた緑内障早期診断法の確立 (2011)
  • Author(s): 嶋澤雅光、原英彰
  • Journal Title: PETジャーナル Volume: 13 Pages: 10-11
• [Journal Article] Lomerizine, Ca^<2+>-channel blocker, protects against neuronal degeneration within the visual center of the brain after retinal damage in mice (2010)
  • Author(s): Ito Y., Nakamura S., Tanaka H., Tsuruma K., Shimazawa M., Araie M. and Hara H.
  • Journal Title: CNS Neuroscience and Therapeutics Volume: 16 Pages: 103-114
• [Journal Article] Involvement of brain-derived neurotrophic factor in time-dependent neurodegeneration in the murine superior colliculus after intravitreal injection of N-methyl-D-aspartate (2009)
  • Author(s): Tanaka H., Ito Y., Nakamura S., Shimazawa M. and Hara H.
  • Journal Title: Mol. Vision Pages: 662-669
• [Presentation] 網膜疾患における小胞体ストレスの関与と新規治療戦略 (2012)
  • Author(s): 嶋澤雅光、原英彰
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-28
• [Presentation] PETおよびMRIを用いた緑内障モデルサルにおける分子脳イメージング (2011)
  • Author(s): 原英彰、嶋澤雅光
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 20110914-17
• [Presentation] 網膜虚血障害の発症機序解明とその治療 (2011)
  • Author(s): 原英彰
  • Organizer: 第13回Japan Glaucoma Council
  • Place of Presentation: 東京
  • Year and Date: 2011-12-10
• [Presentation] Brain imaging in laser-induced ocular hypertensive monkeys using PET and MRI (2011)
  • Author(s): 原英彰
  • Organizer: 浙江大学薬学院招待講演
  • Place of Presentation: 中国、杭州
  • Year and Date: 2011-11-17
• [Presentation] Brain imaging in laser-induced ocular hypertensive monkeys using PET and MRI (2011)
  • Author(s): 原英彰
  • Organizer: 中国薬科大学招待講演
  • Place of Presentation: 中国、南京
  • Year and Date: 2011-11-14
• [Presentation] 網膜・視神経障害に伴う視路変性およびその治療戦略 (2011)
  • Author(s): 嶋澤雅光、原英彰
  • Organizer: 第54回日本神経化学大会
  • Place of Presentation: 金沢
  • Year and Date: 2011-09-26
• [Presentation] 緑内障性視神経症と神経変性疾患の関連 (2011)
  • Author(s): 嶋澤雅光、鶴間一寛、原英彰
  • Organizer: 第115回日本眼科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-07-08
• [Presentation] NMDA誘発視神経障害における小胞体ストレスの関与 (2011)
  • Author(s): 三輪彰範、伊藤保志、鶴間一寛、嶋澤雅光、原英彰
  • Organizer: 第119回日本薬理学会近畿部会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-07-08
• [Presentation] 眼疾患における脳内分子イメージング、大阪医科大学眼科オープンカンファレンスンファレンス (2011)
  • Author(s): 原英彰
  • Organizer: 第127回特別講演
  • Place of Presentation: 高槻
  • Year and Date: 2011-06-16
• [Presentation] Brain imaging in ocular hypertensive monkeys using PET and MRI (2011)
  • Author(s): 原英彰
  • Organizer: 3rd Ocular Diseases & Drug Discovery
  • Place of Presentation: Boston, MA, USA
  • Year and Date: 2011-05-26
• [Presentation] PETを用いた緑内障早期診断法の確立 (2011)
  • Author(s): 嶋澤雅光
  • Organizer: 第5回日本分子イメージング学会学術集会
  • Place of Presentation: ピアザ淡海(滋賀県)
  • Year and Date: 2011-05-22
• [Presentation] 緑内障視神経障害モデル動物における拡散テンソル画像 (2011)
  • Author(s): 林拓也
  • Organizer: 第5回日本分子イメージング学会学術集会
  • Place of Presentation: ピアザ淡海(滋賀県)
  • Year and Date: 2011-05-22
• [Presentation] 緑内障における外側膝状体と大脳皮質の分子イメージング (2011)
  • Author(s): 原英彰
  • Organizer: 第115回日本眼科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-12
• [Presentation] 視神経と中枢の変化 (2010)
  • Author(s): 原英彰
  • Organizer: 第12回Japan Glaucoma Council
  • Place of Presentation: 東京
  • Year and Date: 2010-12-11
• [Presentation] 分子イメージングを用いた緑内障早期診断及び創薬研究 (2010)
  • Author(s): 嶋澤雅光
  • Organizer: 第2回異分野交流研究会
  • Place of Presentation: 岐阜薬科大学(岐阜市)
  • Year and Date: 2010-12-10
• [Presentation] Evaluations of retina and brain imaging in an experimental model for glaucomatous monkeys (2010)
  • Author(s): Hideaki Hara
  • Organizer: Asia-Pacific Joint Glaucoma Congress 2010 Taipei
  • Place of Presentation: Taipei International Convention Center(Taipei, Taiwan)
  • Year and Date: 2010-12-05
• [Presentation] Modeling glaucomatous neurodegeneration using diffusion tensor, imaging (2010)
  • Author(s): Takuya Hayashi
  • Organizer: 2010 World Molecular Imaging Congress
  • Place of Presentation: Kyoto International Conference Center(国立京都国際会館)(京都府)
  • Year and Date: 2010-09-09
• [Presentation] サルPET及びMRIを用いたレーザー照射誘発高眼圧後の脳のイメージング (2010)
  • Author(s): 嶋澤雅光
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2010-03-23
• [Presentation] サル高眼圧モデルにおける視覚中枢の形態学的・経時的変化 (2009)
  • Author(s): 伊藤保志他
  • Organizer: 第115回日本薬理学会近畿部会
  • Place of Presentation: 金沢
  • Year and Date: 2009-06-26
• [Book] Central changes in glaucoma : Neuroscientific study using animal models, In : The Mystery of Glaucoma (2011)
  • Author(s): Imamura K., Shimazawa M., Onoe H., Watanabe Y., Ishii K., Mayama C., Akasaki T., Shimegi S., Sato H., Nakadate K., Hara H. and Araie M.
  • Total Pages: 17
  • Publisher: InTech, Croatia