Ananlysis of the retinal degenerative diseases using iPS cells

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592265
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Keio University
• Principal Investigator: OZAWA Yoko 慶應義塾大学, 医学部, 講師 (90265885)
• Project Period (FY): 2009 – 2011
• Keywords: 網膜 / 視細胞 / 再生 / iPS / 神経保護

Research Abstract

We analyzed the pathogenesis of the retinal degenerative diseases using iPS cell-induced retinal cells. We generated the iPS cell which conserves patient's gene sequences. Retinal cells were induced both from control iPS cells and the patient-derived iPS cells, and analyzed the characteristics of the cells. This research was valuable to clarify the pathogenesis of the retinal degenerative disease, and further study is now required to explore a new therapeutic approach.

Research Products

• [Journal Article] Blockade of vascular adhesion protein-1 attenuates choroidal neovascularization (2012)
  • Author(s): Yoshikawa N, Noda K, Ozawa Y, Tsubota K, Mashima Y, Ishida S
  • Journal Title: Mol Vis Volume: 18 Pages: 593-600
  • URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3298450/?tool=pubmed
  • Peer Reviewed
• [Journal Article] The Vision Van, a Mobile Eye Clinic, Aids Relief Efforts in Tsunami-stricken Areas (2012)
  • Author(s): Oshima CR, Yuki K, Uchida A, Dogru M, Koto T, Ozawa Y, Tsubota K
  • Journal Title: Keio J Med Volume: 61(1) Pages: 10-4
  • URL: http://www.kjm.keio.ac.jp/past/61/1/10.pdf
  • Peer Reviewed
• [Journal Article] Vision preservation during retinal inflammation by anthocyanin-rich bilberry extract : cellular and molecular mechanism (2012)
  • Author(s): Miyake S, Takahashi N, Sasaki M, Kobayashi S, Tsubota K, Ozawa Y
  • Journal Title: Lab Invest Volume: 92(1) Pages: 102-9
  • DOI: doi:10.1038/labinvest.2011.132
  • Peer Reviewed
• [Journal Article] Usage of micronutrient supplement for preventing advanced Age-related Macular Degeneration in Japan (2012)
  • Author(s): Sasaki M, Shinoda H, Koto T, Uchida A, Tsubota K, Ozawa Y
  • Journal Title: Archives of Ophthalmology Volume: 130(2) Pages: 254-5
  • DOI: doi:10.1001/archopthalmol.2011.1368
  • Peer Reviewed
• [Journal Article] Neuroprotective Effects of Lutein in the Retina (2012)
  • Author(s): Ozawa Y, Sasaki M, Takahashi N, Kamoshita M, Miyake S, Tsubota K
  • Journal Title: Current Pharmaceutical Design Volume: 18(2) Pages: 51-6
  • DOI: doi:10.2174/138161212798919101
  • Peer Reviewed
• [Journal Article] The formation of an angiogenic astrocyte template is regulated by the neuroretina in a HIF-1-dependent manner (2011)
  • Author(s): Nakamura-Ishizu A, Kurihara T, Okuno Y, Ozawa Y, Kishi K, Goda N, Tsubota K, Okano H, Suda T, Kubota Y
  • Journal Title: Dev Biol
  • DOI: doi:10.1016/j.ydbio.2011.12.027
  • Peer Reviewed
• [Journal Article] Neuroprotective response after photodynamic therapy : Role of vascular endothelial growth factor (2011)
  • Author(s): Suzuki M, Ozawa Y, Kubota S, Hirasawa M, Miyake S, Noda K, Tsubota K, Kadonosono K, Ishida S
  • Journal Title: J Neuroinflammation Volume: 8 Pages: 176
  • DOI: doi:10.1186/1742-2094-8-176
  • Peer Reviewed
• [Journal Article] Neural degeneration in the retina of the streptozotocin(STZ)-induced type 1 diabetes model (2011)
  • Author(s): Ozawa Y, Kurihara T, Sasaki M, Ban N, Yuki K, Kubota S, Tsubota K
  • Journal Title: Experimental Diabetes Research 2011 Volume: 2011 Pages: 108328
  • DOI: doi:10.1155/2011/108328
  • Peer Reviewed
• [Journal Article] Roles of AMP-Activated Protein Kinase in Diabetes-Induced Retinal Inflammation (2011)
  • Author(s): Kubota S, Ozawa Y, Kurihara T, Sasaki M, Yuki K, Miyake S, Noda K, Ishida S, Tsubota K
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 52(12) Pages: 9142-8
  • DOI: doi:10.1167/iovs.11-8041
  • Peer Reviewed
• [Journal Article] Biological role of lutein in the light-induced retinal degeneration (2011)
  • Author(s): Sasaki M, Yuki K, Kurihara T, Miyake S, Noda K, Kobayashi S, Ishida S, Tsubota K, Ozawa Y
  • Journal Title: J Nutr Biochem
  • DOI: doi:10.1016/j.jnutbio.2011.01.006
  • Peer Reviewed
• [Journal Article] Retinal ganglion cell loss in superoxide dismutase 1 deficiency (2011)
  • Author(s): Yuki K, Ozawa Y, Yoshida T, Kurihara T, Hirasawa M, Ozeki N, Shiba D, Noda K, Ishida S, Tsubota K
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 52(7) Pages: 4143-50
  • DOI: doi:10.1167/iovs.10-6294
  • Peer Reviewed
• [Journal Article] Transcriptional factors associated with epithelial-mesenchymal transition in choroidal neovascularization (2011)
  • Author(s): Hirasawa M, Noda K, Noda S, Suzuki M, Ozawa Y, Shinoda K, Inoue M, Ogawa Y, Tsubota K, Ishida S
  • Journal Title: Mol Vis Volume: 17 Pages: 1222-30
  • URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3102030/?tool=pubmed
  • Peer Reviewed
• [Journal Article] Clinical findings in a choroideremia patient who underwent vitrectomy for retinal detachment associated with macular hole (2011)
  • Author(s): Shinoda H, Koto T, Fujiki K, Murakami A, Tsubota K, Ozawa Y
  • Journal Title: Jpn J Ophthalmol Volume: 55(2) Pages: 169-71
  • DOI: DOI:10.1007/s10384-010-0911-0
  • Peer Reviewed
• [Journal Article] Hydrogen and N-acetyl-L-cysteine rescue oxidative stress-induced angiogenesis in a mouse corneal alkali-burn model (2011)
  • Author(s): Kubota M, Shimmura S, Kubota S, Miyashita H, Kato N, Noda K, Ozawa Y, Usui T, Ishida S, Umezawa K, Kurihara T, Tsubota K
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 52(1) Pages: 427-33
  • DOI: doi:10.1167/iovs.10-6167
  • Peer Reviewed
• [Journal Article] Regulation of posttranscriptional modification as a possible therapeutic approach for retinal neuroprotection (2011)
  • Author(s): Ozawa Y, Kurihara T, Tsubota K, Okano H
  • Journal Title: J Ophthalmol Volume: 2011 Pages: 506137
  • DOI: doi:10.1155/2011/506137
  • Peer Reviewed
• [Journal Article] Resveratrol prevents light-induced retinal degeneration via suppressing activator protein-1 activation (2010)
  • Author(s): Kubota S, Kurihara T, Ebinuma M, Kubota M, Yuki K, Sasaki M, Noda K, Ozawa Y, Oike Y, Ishida S, Tsubota K
  • Journal Title: Am J Pathol Volume: 177(4) Pages: 1725-31
  • DOI: DOI:10.2353/ajpath.2010.100098
  • Peer Reviewed
• [Journal Article] Retinal aging and sirtuins (2010)
  • Author(s): Ozawa Y, Kubota S, Narimatsu T, Yuki K, Koto T, Sasaki M, Tsubota K
  • Journal Title: Ophthalmic Res Volume: 44(3) Pages: 199-203
  • DOI: DOI:10.1159/000316484
  • Peer Reviewed
• [Journal Article] Secondary macular hole formation with presumed evulsion of foveal hard exudates in a patient with diabetic retinopathy (2010)
  • Author(s): Tomita Y, Noda K, Shinoda H, Ozawa Y, Tsubota K, Ishida S
  • Journal Title: Jpn J Ophthalmol Volume: 54(4) Pages: 366-8
  • DOI: DOI:10.1007/s10384-010-0823-
  • Peer Reviewed
• [Journal Article] Eicosapentaenoic acid suppresses ocular inflammation in endotoxin-induced uveitis (2010)
  • Author(s): Suzuki M, Noda K, Kubota S, Hirasawa M, Ozawa Y, Tsubota K, Mizuki N, Ishida S
  • Journal Title: Mol Vis Volume: 16 Pages: 1382-8
  • URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2905642/?tool=pubmed
  • Peer Reviewed
• [Journal Article] von Hippel-Lindau protein regulates transition from the fetal to the adult circulatory system in retina (2010)
  • Author(s): Kurihara T, Kubota Y, Ozawa Y, Takubo K, Noda K, Simon MC, Johnson RS, Suematsu M, Tsubota K, Ishida S, Goda N, Suda T, Okano H
  • Journal Title: Development Volume: 137(9) Pages: 1563-71
  • DOI: doi:10.1242/dev.049015
  • Peer Reviewed
• [Journal Article] Neurodegenerative influence of oxidative stress in the retina of a murine model of diabetes (2010)
  • Author(s): Sasaki M, Ozawa Y, Kurihara T, Kubota S, Yuki K, Noda K, Kobayashi S, Ishida S, Tsubota K
  • Journal Title: Diabetologia Volume: 53(5) Pages: 971-9
  • DOI: DOI:10.1007/s00125-009-1655-
  • Peer Reviewed
• [Journal Article] Mechanism of visual sensations experienced during pars plana vitrectomy under retrobulbar anesthesia (2009)
  • Author(s): Sugisaka E, Shinoda K, Sano RY, Ishida S, Imamura Y, Ozawa Y, Shinoda H, Suzuki K, Tsubota K, Inoue M
  • Journal Title: Ophthalmologica Volume: 224(2) Pages: 103-8
  • DOI: DOI:10.1159/000235858
  • Peer Reviewed
• [Journal Article] Retinal phototoxicity in a novel murine model of intraocular lens implantation (2009)
  • Author(s): Kurihara T, Omoto M, Noda K, Ebinuma M, Kubota S, Koizumi H, Yoshida S, Ozawa Y, Shimmura S, Ishida S, Tsubota K
  • Journal Title: Mol Vis Volume: 15 Pages: 2751-61
  • URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2793903/?tool=pubmed
  • Peer Reviewed
• [Journal Article] Secondary macular hole associated with central retinal vein occlusion treated with corticosteroid injection (2009)
  • Author(s): Hirasawa M, Noda K, Shinoda H, Ozawa Y, Tsubota K, Ishida S
  • Journal Title: Jpn J Ophthalmol Volume: 53(3) Pages: 279-81
  • DOI: DOI:10.1007/s10384-009-0665-8
  • Peer Reviewed
• [Journal Article] Involvement of hyaluronan and its receptor CD44 with choroidal neovascularization (2009)
  • Author(s): Mochimaru H, Takahashi E, Tsukamoto N, Miyazaki J, Yaguchi T, Koto T, Kurihara T, Noda K, Ozawa Y, Ishimoto T, Kawakami Y, Tanihara H, Saya H, Ishida S, Tsubota K
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 50(9) Pages: 4410-5
  • DOI: DOI:10.1167/iovs.08-3044
  • Peer Reviewed
• [Journal Article] (Pro) renin receptor-mediated signal transduction and tissue renin-angiotensin system contribute to diabetes-induced retinal inflammation (2009)
  • Author(s): Satofuka S, Ichihara A, Nagai N, Noda K, Ozawa Y, Fukamizu A, Tsubota K, Itoh H, Oike Y, Ishida S
  • Journal Title: Diabetes Volume: 58(7) Pages: 1625-33
  • DOI: DOI:10.2337/db08-0254
  • Peer Reviewed
• [Journal Article] Prevention of ocular inflammation in endotoxin-induced uveitis with resveratrol by inhibiting oxidative damage and nuclear factor-kappaB activation (2009)
  • Author(s): Kubota S, Kurihara T, Mochimaru H, Satofuka S, Noda K, Ozawa Y, Oike Y, Ishida S, Tsubota K
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 50(7) Pages: 3512-9
  • DOI: DOI:10.1167/iovs.08-2666
  • Peer Reviewed
• [Journal Article] Neuroprotective effect of an antioxidant, lutein, during retinal inflammation (2009)
  • Author(s): Sasaki M, Ozawa Y, Kurihara T, Noda K, Imamura Y, Kobayashi S, Ishida S, Tsubota K
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 50(3) Pages: 1433-9
  • DOI: DOI:10.1167/iovs.08-2493
  • Peer Reviewed
• [Presentation] A gene therapy for a gene mutation in human iPS cell using helper-dependent adenoviral vector (2011)
  • Author(s): Yoshida T, Koizumi H, Yuki K, Hirabayashi Y, Suzuki K, Mitani K, Tsubota K, Shimmura S, Ozawa Y, Okano H
  • Organizer: The Association for Research in Vision and Ophthalmology(ARVO) Annual Meeting
  • Place of Presentation: Fort Lauderdale, FL, USA
  • Year and Date: 20110501-05
• [Presentation] Purification of iPS cells derived from a retinitis pigmentosa patient from feeder cells (2010)
  • Author(s): Yoshida T, Koizumi H, Yuki K, Tsubota K, Okano H, Ozawa Y
  • Organizer: Association for Research in Vision and Ophthalmology(ARVO) Annual Meeting
  • Place of Presentation: Fort Lauderdale, FL, USA
  • Year and Date: 20100502-06
• [Presentation] SOCS3is required to temporally fine-tune photoreceptor cell differentiation (2009)
  • Author(s): Ozawa Y, Nakao K, Tsubota K, Yoshimura A, Okano H
  • Organizer: OIST Retinal Workshop "The Retina : Neural Stem Cells and Photoreceptor Degeneration"
  • Place of Presentation: Okinawa
  • Year and Date: 20091109-11
• [Presentation] Expression of Epiplakin1 in the developing and adult mouse retina (2009)
  • Author(s): Yoshida T, Kume S, Harada T, Tsubota K, Ozawa Y
  • Organizer: OIST Retinal Workshop "The Retina : Neural Stem Cells and Photoreceptor Degeneration"
  • Place of Presentation: Okinawa
  • Year and Date: 20091109-11
• [Remarks]
  • URL: http://www.keio-eye.net/research/rcb.html