2011 Fiscal Year Final Research Report
Basic research about the development of the allogeneic reconstituted skin equivalent which induces local immunotolerance
| Project/Area Number |
21592290
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YOTSUYANAGI Takatoshi 札幌医科大学, 医学部, 教授 (70250595)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 組織培養 / 移植学 |
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| Research Abstract |
Basic research was performed with inbred rat strain to develope the allogeneic reconstituted skin equivalent inducing local immunotolerance.
Keratinocyte and fibroblast was derived from rat skin, and the keratinocyte was immortalized(AK-E7). The reconstituted skin equivalent, using AK-E7 with fibroblast, showed nearly normal histologic epidermal structure. The cultured supernatant of the fibroblast transfected with IL-10 cDNA(AFB-IL10) inhibited mixed lymphocyte reaction. As the reconstituted skin equivalent using AK-E7 and AFB-IL10 was grafted into autograft model, its partial take was recognized histologically.
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