2011 Fiscal Year Final Research Report
Experimental study of chronic skin ulcer re-epithelization by organotypic culture
| Project/Area Number |
21592293
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Dokkyo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ASATO Hirotaka 獨協医科大学, 医学部, 教授 (20222581)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 細胞・組織 / 創傷治癒 |
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| Research Abstract |
Using a three-dimensional skin culture model and a wound healing model, we tried to review difference in epithelium formation and re-epithelizaion process on skin equivalent model consist of fibroblasts which were obtained from normal skin and chronic ulcer. Fibroblasts obtained from chronic ulcer showed poor proliferative capacity, and both epithelium formation and re-epithelizaion process were insufficient. We used a substitute system that we use multiple passaged fibroblasts or hydrocortisone containing culture medium. In a skin equivalent model, the contraction process and the hardness were inhibited. And in a skin culture model and wound healing model, epithelium formation and re-epithelizaion process were restrained.
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