2011 Fiscal Year Final Research Report
Effect of local environment, fibrin, and basic FGF incorporation on a canine autologous model of bio-engineered cartilage tissue
| Project/Area Number |
21592300
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Kinki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ASAMURA Shinichi 近畿大学, 医学部, 准教授 (20340804)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 生分解性高分子 / 自家移植 / 軟骨再生 / bFGF / 徐放システム |
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| Research Abstract |
We developed a technique to form a bioabsorbable synthetic polymer(poly glycolic acid ; PGA) combined with a natural polymer(fibrin) to serve as a scaffold to help retain seeded cells and improve the seeding efficiency of chondrocytes in an implantable construct. This approach was evaluated in a canine autologous implant model of bio-engineered cartilage. The implantation site(subcutaneous or intra-fascial) and the use of basic fibroblast growth factor(b-FGF) were also evaluated with this system. The intra-fascial implantation site yielded optimal results, especially when used in conjunction with fibrin and a b-FGF sustained-release system incorporated into the complex. A thicker, more sustained cartilagenous layer was formed, with a more vascularized outer fibrous supporting tissue layer. This combined approach of implant environment selection, natural polymer for cell retention, and growth factor supplementation offers a more optimized method for generating bio-engineered auricular cartilage.
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