Investigation of consortia formation by periodontopathic bacteria

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592344
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: ISHIHARA Kazuyuki 東京歯科大学, 歯学部, 教授 (00212910)
• Co-Investigator(Kenkyū-buntansha): KATO Tetsuo 東京歯科大学, 微生物学講座, 教授 (00159253) ; KIMIZUKA Ryuta 東京歯科大学, 微生物学講座, 講師 (90287178) ; INAGAKI Satoru 東京歯科大学, 微生物学講座, 助教 (20385165)
• Project Period (FY): 2009 – 2011
• Keywords: 口腔細菌学 / バイオフィルム / 歯周病 / 歯周病原性菌 / Synergistic effect / コンソーシア

Research Abstract

In this project, our goal was to investigate bacterial interaction in the process of biofilm formation to clarify the virulence of periodontopathic bacteria in dental plaque. In the process of colonization, P. gingivalis adhered to T. denticola by means of Hgp44, which is located on its surface. A synergistic effect was observed in co-culture of F. nucleatum and P. intermedia, C. ochracea, or P. gingivalis. In addition, invasion of epithelial cells by P. gingivalis was enhanced by co-infection with F. nucleatum or P. gingivalis. These results suggest that organization as consortia confers an advantage on periodontopathic bacteria in the formation of dental plaque.

Research Products

• [Journal Article] Transmission of Periodontopathic Bacteria from Natural Teeth to Implants (2012)
  • Author(s): Aoki, M., Takanashi, K., Matsukubo, T., Yajima, Y., Okuda, K., Sato, T., Ishihara, K
  • Journal Title: Clin Implant Dent Relat Res Volume: 14 Pages: 406-411
  • DOI: DOI:10.1111/j.1708-8208.2009.00260.x
  • Peer Reviewed
• [Journal Article] Antibacterial activity of povidone-iodine against an artificial biofilm of Porphyromonas gingivalis and Fusobacterium nucleatum (2012)
  • Author(s): Hosaka, Y., Saito, A., Maeda, R., Fukaya, C., Morikawa, S., Makino, A., Ishihara, K., Nakagawa, T
  • Journal Title: Arch Oral Biol Volume: 57 Pages: 364-368
  • DOI: DOI:10.1016/j.archoralbio.2011.09.005
  • Peer Reviewed
• [Journal Article] Detection of periodontal bacteria in thrombi of patients with acute myocardial infarction by polymerase chain reaction (2012)
  • Author(s): Ohki, T., Itabashi, Y., Kohno, T., Yoshizawa, A., Nishikubo, S., Watanabe, S., Yamane, G., Ishihara, K
  • Journal Title: Am Heart J Volume: 163 Pages: 164-167
  • DOI: DOI:10.1016/j.ahj.2011.10.012
  • Peer Reviewed
• [Journal Article] Synergy in biofilm formation between Fusobacterium nucleatum and Prevotella species (2012)
  • Author(s): Okuda, T., Kokubu, E., Kawana, T., Saito, A., Okuda, K., Ishihara, K
  • Journal Title: Anaerobe Volume: 18 Pages: 110-116
  • DOI: DOI:10.1016/j.anaerobe.2011.09.003
  • Peer Reviewed
• [Journal Article] Synergistic effect on biofilm formation between Fusobacterium nucleatum and Capnocytophaga ochracea (2012)
  • Author(s): Okuda, T., Okuda, K., Kokubu, E., Kawana, T., Saito, A., Ishihara, K
  • Journal Title: Anaerobe Volume: 18 Pages: 157-161
  • DOI: DOI:10.1016/j.anaerobe.2012.01.001
  • Peer Reviewed
• [Journal Article] Fimbriae-associated Genes are Biofilm-forming Factors in Aggregatibacter actinomycetemcomitans Strains (2011)
  • Author(s): Saito, T., Ishihara, K., Ryu, M., Okuda, K., Sakurai, K
  • Journal Title: Bull Tokyo Dent Coll Volume: 51 Pages: 145-150
  • DOI: DOI:JST.JSTAGE/tdcpublication/51.145 [pii]
  • Peer Reviewed
• [Journal Article] Funoran-containing Xylitol gum and tablets inhibit adherence of oral streptococci (2011)
  • Author(s): Inagaki, S., Saeki, Y., Ishihara, K
  • Journal Title: J Oral Biosci Volume: 53 Pages: 82-86
  • URL: https://www.jstage.jst.go.jp/article/joralbiosci/53/1/53_1_82/_pdf
  • Peer Reviewed
• [Journal Article] Effect of smoking on subgingival microflora of patients with periodontitis in Japan (2011)
  • Author(s): Kubota, M., Tanno-Nakanishi, M., Yamada, S., Okuda, K., Ishihara, K
  • Journal Title: BMC Oral Health Volume: 11 Pages: 1
  • DOI: DOI:10.1186/1472-6831-11-1
  • Peer Reviewed
• [Journal Article] HGP44 induces protection against Porphyromonas gingivalis-induced alveolar bone loss in mice (2011)
  • Author(s): Muramatsu, K., Kokubu, E., Shibahara, T., Okuda, K., Ishihara, K
  • Journal Title: Clin Vaccine Immunol Volume: 18 Pages: 888-891
  • DOI: DOI:10.1128/CVI.00556-10
  • Peer Reviewed
• [Journal Article] Exposure of P. gingivalis to noradrenaline reduces bacterial growth and elevates ArgX protease activity (2011)
  • Author(s): Saito, T., Inagaki, S., Sakurai, K., Okuda, K., Ishihara, K.
  • Journal Title: Arch Oral Biol Volume: 56 Pages: 244-250
  • DOI: DOI:10.1016/j.archoralbio.2010.09.014
  • Peer Reviewed
• [Journal Article] The inhibition of infection by wound pathogens on scaffold in tissue-forming process using N-acetyl cysteine (2011)
  • Author(s): Yamada, M., Ishihara, K., Ogawa, T., Sakurai, K
  • Journal Title: Biomaterials Volume: 32 Pages: 8474-8485
  • DOI: DOI:10.1016/j.biomaterials.2011.07.074
  • Peer Reviewed
• [Journal Article] Colonization of the oral cavity by periodontopathic bacteria in complete denture warers (2011)
  • Author(s): Yasui, M., Ryu, M., Sakurai, K., Ishihara, K
  • Journal Title: Gerodontology
  • DOI: DOI:10.1111/j.1741-2358.2011.005.x
  • Peer Reviewed
• [Journal Article] Oral bacterial extracts facilitate early osteogenic/dentinogenic differentiation in human dental pulp-derived cells (2010)
  • Author(s): Abe, S., Imaizumi, M., Mikami, Y., Wada, Y., Tsuchiya, S., Irie, S., Suzuki, S., Satomura, K., Ishihara, K., Honda, M. J
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Volume: 109 Pages: 149-154
  • DOI: DOI:10.1016/j.tripleo.2009.08.028
  • Peer Reviewed
• [Journal Article] Virulence factors of Treponema denticola (2010)
  • Author(s): Ishihara, K
  • Journal Title: Periodontol 2000 Volume: 54 Pages: 117-135
  • DOI: DOI:10.1111/j.1600-0757.2009.00345.x
  • Peer Reviewed
• [Journal Article] Dentipain, a Streptococcus pyogenes IdeS protease homolog, is a novel virulence factor of Treponema denticola (2010)
  • Author(s): Ishihara, K., Wawrzonek, K., Shaw, L. N., Inagaki, S., Miyamoto, M., Potempa, J
  • Journal Title: Biological chemistry Volume: 391 Pages: 1047-1055
  • DOI: DOI:10.1515/BC.2010.113
  • Peer Reviewed
• [Journal Article] Hemagglutinin/Adhesin domains of Porphyromonas gingivalis play key roles in coaggregation with Treponema denticola (2010)
  • Author(s): Ito, R., Ishihara, K., Shoji, M., Nakayama, K., Okuda, K
  • Journal Title: FEMS Immunol Med Microbiol Volume: 60 Pages: 251-260
  • DOI: DOI:10.1111/j.1574-695X.2010.00737.x
  • Peer Reviewed
• [Journal Article] Investigation of subgingival profile of periodontopathic bacteria using polymerase chain reaction (2010)
  • Author(s): Komiya-Ito, A., Ishihara, K., Tomita, S., Kato, T., Yamada, S
  • Journal Title: Bull Tokyo Dent Coll Volume: 51 Pages: 139-144
  • DOI: DOI:JST.JSTAGE/tdcpublication/51.139 [pii]
  • Peer Reviewed
• [Journal Article] Basic peptide protamine exerts antimicrobial activity against periodontopathic bacteria (2010)
  • Author(s): Miura, T., Iohara, K., Kato, T., Ishihara, K., Yoshinari, M
  • Journal Title: J Biomed Sci Eng Volume: 3 Pages: 1069-1072
  • DOI: DOI:10.4236/jbise.2010.311138
  • Peer Reviewed
• [Journal Article] Oral environmental factors affecting number of microbes in saliva of complete denture wearers (2010)
  • Author(s): Ryu, M., Ueda, T., Saito, T., Yasui, M., Ishihara, K., Sakurai, K
  • Journal Title: J Oral Rehabil Volume: 37 Pages: 194-201
  • DOI: DOI:10.1111/j.1365-2842.2009.02042.x
  • Peer Reviewed
• [Journal Article] Analysis of matrix metalloproteinase(MMP-8 and MMP-2) activity in gingival crevicular fluid from children with Down's syndrome (2010)
  • Author(s): Yamazaki-Kubota, T., Miyamoto, M., Sano, Y., Kusumoto, M., Yonezu, T., Sugita, K., Okuda, K., Yakushiji, M., Ishihara, K
  • Journal Title: J Periodontal Res Volume: 45 Pages: 170-176
  • DOI: DOI:10.1111/j.1600-0765.2009.01214.x
  • Peer Reviewed
• [Journal Article] Characterization of bacterial flora in persistent apical periodontitis lesions (2009)
  • Author(s): Fujii, R., Saito, Y., Tokura, Y., Nakagawa, K. I., Okuda, K., Ishihara, K
  • Journal Title: Oral Microbiol Immunol Volume: 24 Pages: 502-505
  • DOI: DOI:10.1111/j.1399-302X.2009.00534.x
  • Peer Reviewed
• [Journal Article] Inhibitory effect of galectin-3 on the cytokine-inducing activity of periodontopathic Aggregatibacter actinomycetemcomitans endotoxin in splenocytes derived from mice (2009)
  • Author(s): Kato, T., Uzawa, A., Ishihara, K
  • Journal Title: FEMS Immunol Med Microbiol Volume: 57 Pages: 40-45
  • DOI: DOI:10.1111/j.1574-695X.2009.00577.x
  • Peer Reviewed
• [Journal Article] Differential ability of periodontopathic bacteria to modulate invasion of human gingival epithelial cells by Porphyromonas gingivalis (2009)
  • Author(s): Saito, A., Inagaki, S., Ishihara, K
  • Journal Title: Microb Pathog Volume: 47 Pages: 329-333
  • DOI: DOI:10.1016/j.micpath.2009.09.012
  • Peer Reviewed
• [Presentation] Behavior of epithelial rests of Malassez cell infected by Treponema denticola (2012)
  • Author(s): Kokubu E, Inagaki S, Kimizuka R, Ishihara K
  • Organizer: 第85回日本細菌学会総会、長崎
  • Place of Presentation: 長崎
  • Year and Date: 20120327-29
• [Presentation] Characterization of bacteriocin associated protein in Treponema denticola (2012)
  • Author(s): Tanaka K, Inagaki S, Shintani S, Ishihara K
  • Organizer: 第85回日本細菌学会総会、長崎
  • Place of Presentation: 長崎
  • Year and Date: 20120327-29
• [Presentation] Treponema denticolaの細胞侵入に対するMalassez上皮遺残細胞の細胞動態 (2011)
  • Author(s): 国分栄仁, 稲垣覚, 君塚隆太, 石原和幸
  • Organizer: 第53回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 岐阜
  • Year and Date: 20110930-1002
• [Presentation] Identification and characterization of potential solute-binding protein of Treponema denticola (2011)
  • Author(s): Asai T, Nakagawa K-I, Ishihara K
  • Organizer: XIII International Congress for Bacteriology and Applied Microbiology
  • Place of Presentation: Sapporo
  • Year and Date: 20110906-10
• [Presentation] Behavior of epithelial rests of Malassez cells infected by Treponema denticola (2011)
  • Author(s): Kokubu E, Inagaki S, Kimizuka R, Ishihara K
  • Organizer: XIII International Congress for Bacteriology and Applied Microbiology
  • Place of Presentation: Sapporo
  • Year and Date: 20110906-10
• [Presentation] Periodontopathic biofilm formation of Fusobacterium nucleatum with Prevotella intermedia, Prevotella nigrescens or Capnocytophaga ochracea (2011)
  • Author(s): Okuda T, Kokubu E, Saito A, Okuda K, Ishihara K
  • Organizer: XIII International Congress for Bacteriology and Applied Microbiology
  • Place of Presentation: Sapporo
  • Year and Date: 20110906-10
• [Presentation] Treponema denticolaの細胞侵入に対するMalassez上皮遺残細胞の細胞動態 (2010)
  • Author(s): 国分栄仁, 稲垣覚, 君塚隆太, 石原和幸
  • Organizer: 第52回歯科基礎医学会学術大会・総会
  • Place of Presentation: 東京
  • Year and Date: 20100920-22
• [Presentation] Treponema denticolaのIdeS類似システインプロテアーゼdentipainの解析 (2010)
  • Author(s): 石原和幸, 稲垣覚
  • Organizer: 第52回歯科基礎医学会学術大会・総会
  • Place of Presentation: 東京
  • Year and Date: 20100920-22
• [Presentation] Treponema denticola invasion of human gingival epithelial cells (2010)
  • Author(s): Inagaki S, Saito A, Kimizuka R, Ishihara K
  • Organizer: 88 th General session & exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20100712-17
• [Presentation] Differential ability of periodontopathogens to modulate Porphyromonas gingivalis host invasion (2010)
  • Author(s): Saito A, Inagaki S, Kokubu E, Ishihara K
  • Organizer: 88 th General session & exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20100712-17
• [Presentation] 歯周病原性菌と心血管系疾患 (2010)
  • Author(s): 石原和幸
  • Organizer: 第83回日本細菌学会
  • Place of Presentation: 横浜
  • Year and Date: 20100327-29
• [Presentation] Treponema denticola invasion of human gingival epithelial cells (2010)
  • Author(s): Ishihara K, Inagaki S, Kimizuka R
  • Organizer: Godon Conference, Spirochetes, Biology of Jan.
  • Year and Date: 20100131-0206
• [Presentation] 細菌の生残戦略をめぐるあらたな展開複数菌種によるバイオフィルム形成機構 (2009)
  • Author(s): 石原和幸
  • Organizer: 第82回日本生化学会
  • Place of Presentation: Kobe
  • Year and Date: 20091021-24
• [Presentation] Invasin of host cells by periodontopathic bacteria In polymicrobial infection (2009)
  • Author(s): Saito A, Inagaki S, Kimizuka R, Ishihara K
  • Organizer: 87 th General session & exhibition of the International Association for Dental Research
  • Place of Presentation: Miami, USA
  • Year and Date: 20090401-04
• [Presentation] Porphyromonas gingivalis enhances outer membrane protein expression of Fusobacterium nucleatum (2009)
  • Author(s): Yamaguchi Y, Fujii R, Asai T, Ito R, Nakagawa K-I, Ishihara K
  • Organizer: 87 th General session & exhibition of the International Association for Dental Research
  • Place of Presentation: Miami, USA
  • Year and Date: 20090401-04
• [Presentation] 周病原菌Treponema denticolaのヒト歯肉上皮細胞への侵入 (2009)
  • Author(s): 稲垣覚, 齋藤淳, 君塚隆太, 石原和幸
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 20090312-14
• [Book] Invasion of Host Cells by Porphyromonas Gingivalis in Polymicrobial Infection, Periodontal Disease : Symptoms, Treatment and Prevention (2011)
  • Author(s): Saito, A., Inagaki, S., Kokubu, E., Kimizuka, R., Ishihara, K, Yamamoto, S. L.
  • Total Pages: 257-271
  • Publisher: Nova Science Publishers, Inc.
• [Book] Chapter 44.Capnocytophaga, In : Liu, D., ed. Molecular Detection Of Human Bacterial Pathogens (2011)
  • Author(s): Ishihara, K., Inagaki, S., Saito, A
  • Publisher: CRC press