2012 Fiscal Year Final Research Report
Study of relationship of physiological roles and pathogenicity of aggressive periodontopathic bacterial quinol peroxidase
| Project/Area Number |
21592346
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2012
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| Keywords | 口腔細菌学 |
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| Research Abstract |
Quinol peroxidase (QPO) of Aggregatibacter actinomycetemcomitans catalyzes peroxidase activity using a quinol on respiratory chain.Aqpo null mutant exhibits an oxidative stress phenotype, suggesting that QPO plays animportant role in scavenging endogenously generated hydrogen peroxide, and exhibitsreduced production of leukocyte (LtxA), a secreted bacterial toxin that is known to targethuman leukocytes. Screening identified ascofuranone as a highly potent inhibitor of QPO(Ki = 9.6 nM). This inhibitor also reduced the secretion of LtxA, with an associatedreduction in the pathogenicity ofA. actinomycetemcomitans for HL-60 cells. We producedthe recombinant QPO expression system inEscherichia coli. Purified recombinant QPOwas used to analyse the catalytic mechanism, indicating that QPO catalyzes as ping pongbi bi mechanism.
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Research Products
(7 results)
