Synaptic Inputs Switching Functional Modes in Primary Sensory Neurons of Rat Trigeminal Mesencephalic Nucleus

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592358
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Osaka University
• Principal Investigator: SAITO Mitsuru 大阪大学, 大学院・歯学研究科, 講師 (50347770)
• Project Period (FY): 2009 – 2011
• Keywords: 三叉神経中脳路核 / 一次感覚ニューロン / 介在ニューロン / 機能モード / AMPA型グルタミン酸受容体チャネル / hチャネル / Na^+マイクロドメイン / 咀嚼運動

Research Abstract

Since primary sensory neurons(PSNs) in the mesencephalic trigeminal nucleus(MTN) are unique in receiving synaptic inputs onto various types of receptors such as a glutamatergic type-I AMPA receptor channel(AMPARCh), MTN neurons are thought to act not only as PSNs but also but also as interneurons independent of the activities of the peripheral receptors(Saito et al., 2006). In this study, we have demonstrated that in the rat MTN neurons, enhancement of h-current, caused by hyperpolarization of holding potential, activation of leak K^+ current or PKA activation, suppresses AMPARCh-mediated currents and might switch the MTN neuron to the PSN function mode.

Research Products

• [Journal Article] 【口腔領域をめぐる神経基盤】噛みしめ運動における咬筋運動ニューロン序列動員の階層性制御機構 (2011)
  • Author(s): 姜英男, 齋藤充, 豊田博紀, 佐藤元
  • Journal Title: 脳21 Volume: 14 Pages: 385-301
  • Peer Reviewed
• [Journal Article] Rank-ordered recruitment of masseter motoneurons by the activity of mesencephalic trigeminal neurons during slow closing phase of mastication cycle (2010)
  • Author(s): Kang Y, Saito M, Toyoda H, Sato H.
  • Journal Title: Journal of Oral Bioscience Volume: 52 Pages: 330-335
  • Peer Reviewed
• [Journal Article] Protein Kinase G Dynamically Modulates TASK1-Mediated Leak K+ Currents in Cholinergic Neurons of the Basal Forebrain (2010)
  • Author(s): Toyoda H*, Saito M*, Okazawa M*, Hirao K, Sato H, Abe H, Takada K, Funabiki K, Takada M, Kaneko T, Kang Y.
  • Journal Title: The Journal of Neuroscience Volume: 30 Pages: 5677-5689
  • Peer Reviewed
• [Journal Article] Occlusal-masticatory function and learning and memory : Immunohistochemical, biochemical, behavioral and electrophysiological studies in rats (2010)
  • Author(s): Hirai T, Kang Y, Koshino H, Kawanishi K, Toyoshita Y, Ikeda Y, Saito M.
  • Journal Title: Japanese Dental Science Review Volume: 46 Pages: 143-149
  • Peer Reviewed
• [Journal Article] Rapid use-dependent down-regulation of gamma-aminobutyric acid type A receptors in rat mesencephalic trigeminal neurons (2009)
  • Author(s): Saito M, Toyoda H, Sato H, Ishii H, Kang Y.
  • Journal Title: Journal of Neuroscience Research Volume: 87 Pages: 3120-3133
  • Peer Reviewed
• [Presentation] cGMP differentially modulates leak K^+ currents in masseter motoneurons (2011)
  • Author(s): Toyoda H, Hirao K, Saito M, Sato H, Kang Y.
  • Organizer: The 41st Annual Meeting of Society for Neuroscience
  • Place of Presentation: Walter E. Washington Convention Center, Washington, DC, USA.
  • Year and Date: 2011-11-13
• [Presentation] TASK1チャネル抗体の作製および咬筋運動ニューロンにおけるTASK1/ 3チャネル発現分布の検討 (2011)
  • Author(s): 榎村徳仁, 齋藤充, 豊田博紀, 佐藤元, 姜英男
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 長良川国際会議場,岐阜
  • Year and Date: 2011-10-02
• [Presentation] cGMPによるラット閉口筋運動ニューロンの興奮性の修飾 (2011)
  • Author(s): 深津雄己, 豊田博紀, 齋藤充, 佐藤元, 姜英男
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 長良川国際会議場,岐阜
  • Year and Date: 2011-10-02
• [Presentation] 閉口筋運動ニューロンの序列動員とcGMPによるその修飾 (2011)
  • Author(s): 佐藤元, 深津雄己, 豊田博紀, 齋藤充, 姜英男
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 長良川国際会議場,岐阜
  • Year and Date: 2011-10-01
• [Presentation] Intercolumnar and intracolumnar desynchronization by GABAB receptor-mediated presynaptic inhibition in the rat barrel cortex (2011)
  • Author(s): Saito M, Sato H, Toyoda H, Tanaka T, Kobayashi M, Althof D, Kulik A, Aoyagi T, Shigemoto R, Kang Y.
  • Organizer: The 88th Annual Meeting of the Physiological Society of Japan & The 116th Annual Meeting of the Japanese Association of Anatomists
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
  • Year and Date: 2011-03-28
• [Presentation] Bidirectional regulation of TASK1 channels expressed in HEK cells by modulation of PKG (2011)
  • Author(s): Toyoda H, Saito M, Okazawa M, Hirao K, Sato H, Abe H, Takada K, Funabiki K, Takada M, Kaneko T, Kang Y.
  • Organizer: The 88th Annual Meeting of the Physiological Society of Japan & The 116th Annual Meeting of the Japanese Association of Anatomists
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
  • Year and Date: 2011-03-28
• [Presentation] Differential electrophysiological properties of capsaicin-induced currents between layer II/ III and layer V pyramidal cells of the insular cortex (2011)
  • Author(s): Sato H, Kawakami S, Toyoda H, Saito M, Bae YC, Kang Y.
  • Organizer: The 88th Annual Meeting of the Physiological Society of Japan & The 116th Annual Meeting of the Japanese Association of Anatomists
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
  • Year and Date: 2011-03-28
• [Presentation] Membrane hyperpolarization depresses AMPA currents in the primary sensory neuron of the rat mesencephalic trigeminal nucleus (2010)
  • Author(s): Kawasaki Y, Saito M, Toyoda H, Sato H, Tanaka S, Kogo M, Kang Y.
  • Organizer: The 40th Annual Meeting of Society for Neuroscience
  • Place of Presentation: San Diego Convention Center, San Diego, USA.
  • Year and Date: 2010-11-17
• [Presentation] Interand intracolumnar desynchronization by presynaptic GABAB inhibition in the rat barrel cortex (2010)
  • Author(s): Saito M, Sato H, Toyoda H, Kobayashi M, Althof D, Kulik A, Shigemoto R, Kang Y.
  • Organizer: The 40th Annual Meeting of Society for Neuroscience
  • Place of Presentation: San Diego Convention Center, San Diego, USA.
  • Year and Date: 2010-11-15
• [Presentation] Kang Y Protein kinase G(PKG) bidirectionally modulates TASK1 currents in PKG-loaded HEK 293 cells (2010)
  • Author(s): Toyoda H, Saito M, Okazawa M, Hirao K, Sato H, Abe H, Takada K, Funabiki K, Takada M, Kaneko T
  • Organizer: The 40th Annual Meeting of Society for Neuroscience
  • Place of Presentation: San Diego Convention Center, San Diego, USA.
  • Year and Date: 2010-11-15
• [Presentation] Electrophysiological properties of capsaicin-induced currents in layer II/ III and layer V pyramidal cells of the insular cortex (2010)
  • Author(s): Sato H, Kawakami S, Toyoda H, Saito M, Bae YC, Kang Y.
  • Organizer: The 40th Annual Meeting of Society for Neuroscience
  • Place of Presentation: San Diego Convention Center, San Diego, USA.
  • Year and Date: 2010-11-15
• [Presentation] 三叉神経中脳路核ニューロンにおいてグルタミン酸作動性入力を無効化するイオン機構 (2010)
  • Author(s): 川嵜康大, 齋藤充, 佐藤元, 豊田博紀, 姜英男
  • Organizer: 第103回近畿生理学談話会
  • Place of Presentation: 大阪大学銀杏会館,吹田
  • Year and Date: 2010-10-02
• [Presentation] カプサイシン投与により島皮質において誘発されるnetwork oscillatioN (2010)
  • Author(s): 佐藤元, 豊田博紀, 齋藤充, 姜英男
  • Organizer: 第103回近畿生理学談話会
  • Place of Presentation: 大阪大学銀杏会館,吹田
  • Year and Date: 2010-10-02
• [Presentation] Interand intra-columnar desynchronization of excitatory input by presynaptic GABAB inhibition in the rat barrel cortex (2009)
  • Author(s): Saito M, Sato M, Toyoda H, Kang Y.
  • Organizer: The 39th Annual Meeting of Society for Neuroscience
  • Place of Presentation: McCormick Place Convention Center, Chicago, USA.
  • Year and Date: 2009-10-21
• [Presentation] PKG modulates the recruitment of masseter motoneurons caused by the presumed spindle Ia inputs (2009)
  • Author(s): Sato M, Saito M, Toyoda H, Kang, Y.
  • Organizer: The 39th Annual Meeting of Society for Neuroscience
  • Place of Presentation: McCormick Place Convention Center, Chicago, USA.
  • Year and Date: 2009-10-20
• [Presentation] Recruitment of masseter motoneurons by the presumed spindle Ia inputs (2009)
  • Author(s): Sato H, Saito M, Toyoda H, Kang Y.
  • Organizer: The 36th International Congress of Physiological Sciences
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2009-07-30
• [Presentation] Presynaptic GABA_B inhibition involved in the inter-and intra-columnar desynchronization of glutamatergic input in the rat barrel cortex (2009)
  • Author(s): Saito M, Sato H, Toyoda H, Kang Y.
  • Organizer: The 36th International Congress of Physiological Sciences
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2009-07-29
• [Book] Breathe, Walk and Chew. The Neural Challenge : Part I(Progress in Brain Research, Vol.187) (2010)
  • Author(s): Kang Y, Saito M, Toyoda H, Sato H.
  • Total Pages: 163-172
  • Publisher: Elsevier, Amsterdam
• [Book] Interface Oral Health Science 2009 (2010)
  • Author(s): Kang Y, Toyoda H, Saito M, Sato H.
  • Total Pages: 60-68
  • Publisher: Springer, Tokyo
• [Remarks]
  • URL: http://web.dent.osaka-u.ac.jp/~phys/