Role of a ubiquitin ligase for Sox9 on endochondral ossification and neurological disorder

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592359
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Okayama University
• Principal Investigator: HATTORI Takako 岡山大学, 大学院・医歯薬学総合研究科, 助教 (00228488)
• Co-Investigator(Kenkyū-buntansha): TAKIGAWA Masaharu 岡山大学, 大学院・医歯薬学総合研究科, 教授 (20112063) ; KUBOTA Satoshi 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (90221936) ; NISHIDA Takashi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (30322233) ; AOYAMA Eriko 岡山大学, 大学院・医歯薬学総合研究科, 助教 (10432650)
• Project Period (FY): 2009 – 2011
• Keywords: Sox9 / ユビキチン / プロテオミクス解析 / 内軟骨性骨形成 / 神経疾患

Research Abstract

Sox9 is a master regulatory transcription factor of the SRY gene family regulating chondrogenesis as well as neural development. In this report, we identified a ubiquitin ligase which binds specifically and regulates cellular concentration of Sox9, and examined the role of the ubuquitin ligase on endochondral ossification as well as neurological disorder through controlling cellular concentration of Sox9.

Research Products

• [Journal Article] The Wnt antagonist Wif-1 interacts with CTGF and inhibits CTGF activity. (2011)
  • Author(s): Surmann-Schmitt, C., Sasaki, T., Hattori, T., Eitzinger, N., Schett, G., von der Mark, K., and Stock, M.
  • Journal Title: J Cell Physiol. Volume: (in press)
  • Peer Reviewed
• [Journal Article] The dimerization domain of SOX9 is required for transcription activation of a chondrocyte-specific chromatin DNA template (2010)
  • Author(s): Coustry, Francoise ; Oh, Chundo ; Hattori, Takako ; MAITY, SANKAR ; de Crombrugghe, Benoit and Yasuda, Hideyo
  • Journal Title: Nucleic Acids Research Volume: 38 Pages: 6018-6028
  • Peer Reviewed
• [Journal Article] Sox9 is a major negative regulator of cartilage vascularisation, bone marrow formation, and endochondral ossification. (2010)
  • Author(s): Takako Hattori, Catharina Mueller, Sonja Gebhard, Eva Bauer, Friederike Pausch, Britta Schlund, Michael Boesl, Andreas Hess, Cordula Surmann-Schmitt, Helga von der Mark, Benoit de Crombrugghe, Klaus von der Mark.
  • Journal Title: Development Volume: 137 Pages: 901-911
  • Peer Reviewed
• [Journal Article] N-terminal domains of CCN protein 2/connective tissue growth factor bind to aggrecan (2009)
  • Author(s): Aoyama E, Hattori T, Hoshijima M, Araki D, Nishida T, Kubota S, Takigawa M.
  • Journal Title: Biochem J. Volume: 420(3) Pages: 413-20
  • Peer Reviewed
• [Presentation] CCN2/CTGF-CCN3/NOVおよびCCN2-CCN2のダイマー形成が軟骨細胞の分化機能に及ぼす役割 (2012)
  • Author(s): 星島光博、服部高子、, 他
  • Organizer: 第25回日本軟骨代謝学会
  • Place of Presentation: 名古屋
  • Year and Date: 20120309-10
• [Presentation] 結合組織成長因子CCN2/CTGFは頭部神経堤由来の軟骨細胞分化に必須である (2011)
  • Author(s): 服部高子、, 他
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 20111213-16
• [Presentation] The low-density lipoprotein receptor related protein 1(LRP1) deficiency in the cartilage tissue leads to skeletal dysmorphisms (2011)
  • Author(s): Kawata, K., Kubota, S., Hattori, T., et al.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111213-16
• [Presentation] Characterization of the effect of CCN2 modules independently and in different combinations on chondrocytic cells (2011)
  • Author(s): Abd El Kader, T., Kubota, S., Nishida, T., Hattori, T., et al.
  • Organizer: A meeting sponsored by the International CCN society
  • Place of Presentation: Vancouver, BC, Canada
  • Year and Date: 20110924-27
• [Presentation] Assessment of combinational effect of independent CCN2 modules on chondrocytic cells (2011)
  • Author(s): Abd El Kdaer, T., Kubota, S., Nishida, T., Hattori, 他
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会議場、京都
  • Year and Date: 20110921-24
• [Presentation] Roles of the heterotypic CCN2/CTGF-CCN3/NOV and homotypic CCN2-CCN2 interactions in matrix synthesis in chondrocytes (2011)
  • Author(s): Hoshijima, M., Hattori, T., et al.
  • Organizer: The 33th Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: San Diego, California
  • Year and Date: 20110916-20
• [Presentation] Harmine, an inducer of CCN2/CTGF, promotes chondrogenesis and suppresses TNF-α-induced inflammatory response (2011)
  • Author(s): Emilio Satoshi Hara, Mitsuaki Ono, Satoshi Kubota, Wataru Sonoyama, Takako Hattori, 他
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場,大阪
  • Year and Date: 20110728-30
• [Presentation] Evaluation of independent and combinational effect of CCN2 modules on chondrocytic cells (2011)
  • Author(s): Tarek Abd El Kdaer, Satoshi Kubota, Takashi Nishida, Takako Hattori, 他
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場,大阪
  • Year and Date: 20110728-30
• [Presentation] CCN2/CTGFのホモダイマー形成、およびCCN3/NOVとのヘテロダイマーの形成が軟骨細胞の基質合成に及ぼす役割 (2011)
  • Author(s): 星島光博、服部高子、, 他
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場,大阪
  • Year and Date: 20110728-30
• [Presentation] Cartilage-specific overexpression of CCN2/CTGF protects articular cartilage from age-related osteoarthritis-like changes (2011)
  • Author(s): Shinsuke Itoh, Takako Hattori, et al.
  • Organizer: The 2011 Gordon Conference on Cartilage Biology and Pathology
  • Place of Presentation: Ventura, CA
  • Year and Date: 20110306-11
• [Presentation] Cartilage-specific overexpression of CCN2/CTGF protects articular cartilage from age-related degenerative changes (2011)
  • Author(s): Shinsuke Itoh, Takako Hattori, et al.
  • Organizer: 連携機能を活用した口腔からQOL向上を目指す研究「国際シンポジウム」
  • Place of Presentation: ホテルオークラ新潟,新潟
  • Year and Date: 20110209-10
• [Presentation] CCN2/CTGFはマトリリン-3と結合して軟骨マトリックス成分のネットワーク形成を促進する (2010)
  • Author(s): 服部高子、, 他
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド,神戸
  • Year and Date: 20101207-10
• [Presentation] Cartilage-specific overexpression of ccn2/ctgf protects articular cartilage from age-related osteoarthritis-like changes (2010)
  • Author(s): Shinsuke Itoh, Takako Hattori, et al.
  • Organizer: The sixth International Workshop on the CCN Family of Genes
  • Place of Presentation: Slieve Donard Hotel, Newcastle, Northern Ireland
  • Year and Date: 20101020-24
• [Presentation] CCN2/CTGF Interacts with Matrilin-3 and Enhances Assembly of a Cartilage Matrix Protein and Filamentous Network (2010)
  • Author(s): Takako Hattori, et al.
  • Organizer: The sixth International Workshop on the CCN Family of Genes
  • Place of Presentation: Slieve Donard Hotel, Newcastle, Northern Ireland
  • Year and Date: 20101020-24
• [Presentation] Exportin-1によるCCN2/CTGFの核-細胞質間分子輸送とその生理的意義 (2010)
  • Author(s): 内田瑶子、服部高子、, 他
  • Organizer: 第52回歯科基礎医学会総会
  • Place of Presentation: タワーホール船越、東京
  • Year and Date: 20100920-22
• [Presentation] CCN2/CTGFはマトリリン. 3と結合して軟骨マトリックス成分のネットワーク形成を促進する (2010)
  • Author(s): 服部高子、, 他
  • Organizer: 第42回日本結合組織学会学術大会・第57回マトリックス研究会大会合同学術集会
  • Place of Presentation: 秋田拠点センター・アルヴェ、秋田
  • Year and Date: 20100819-20
• [Presentation] CCN2/CTGFの核内移行とExportin-1による核.細胞質間分子輸送 (2010)
  • Author(s): 服部高子、, 他
  • Organizer: 第28回日本骨代謝学会
  • Place of Presentation: 京王プラザホテル、東京
  • Year and Date: 20100721-23
• [Presentation] Suppression of BMP-2-induced osteogenic differentiation of hBMSCs by TNF-α (2010)
  • Author(s): T. Fujisawa, M. Ueda, M. Ono, T. Hattori, et al.
  • Organizer: The International Association for Dental Research
  • Place of Presentation: Centre Convencions Internacional Barcelona, Barcelona, Spain
  • Year and Date: 20100714-17
• [Presentation] Sox9は血管侵入、骨髄形成および内軟骨性骨形成の最終段階を抑制する. Col10a1-BACトランスジェニックマウスを用いた解析- (2010)
  • Author(s): 服部高子、, 他
  • Organizer: 第23回日本軟骨代謝学会,シンポジウム「軟骨分化の分子生物学」
  • Place of Presentation: 鹿児島県医師会館、鹿児島
  • Year and Date: 20100402-03
• [Presentation] Sox9 is a major negative regulator of cartilage vascularization (2009)
  • Author(s): Takako Hattori, et al.
  • Organizer: 2009 Gordon Conference on Cartilage Biology and Pathology
  • Place of Presentation: Switzerland
  • Year and Date: 20090607-12
• [Remarks]
  • URL: http://www.dent.okayama-u.ac.jp/seika/index_sc_j.html
• [Patent(Industrial Property Rights)] がんの治療又は予防剤 (2012)
  • Inventor(s): 滝川正春、服部高子、他
  • Industrial Property Rights Holder: 滝川正春、服部高子、他
  • Industrial Property Number: 特許
  • Filing Date: 2012-01-12
• [Patent(Industrial Property Rights)] アポトーシス誘導剤と併用されるがんの治療又は予防剤 (2012)
  • Inventor(s): 滝川正春、服部高子、他
  • Industrial Property Rights Holder: 滝川正春、服部高子、他
  • Industrial Property Number: 特許
  • Filing Date: 2012-01-12