REGULATION OF WATER TRANSPORT VIA PARACELLULAR PATHWAY BY PHOSPHORYLATION AND DEPHOSPHORYLATION IN SALIVARY GLASNDS

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592375
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Nihon University
• Principal Investigator: SUGIYA Hiroshi 日本大学, 生物資源科学部, 教授 (20050114)
• Co-Investigator(Renkei-kenkyūsha): NARITA Takanori 日本大学, 生物資源科学部, 助教 (70453884)
• Project Period (FY): 2009 – 2011
• Keywords: 顎下腺 / 水分泌 / 傍細胞輸送 / カルシウムイオン / タイト結合 / 翻訳後修飾 / ニューロキニンA / IGF-I

Research Abstract

The relationship between neurokinin A-or pilocarpine-provoked salivary fluid secretion and paracellular transport by using Lucifer Yellow was investigated in perfused rat submandibular gland. Salivary fluid secretion provoked by neurokinin A-or pilocarpine was dependent on the increase in intracellular Ca^<2+> concentrations, which was linked to Lucifer Yellow secretion. These results suggest that Ca^<2+>-dependent phosphorylation and/or dephosphorylation appears to contribute to the paracellular transport. On the other hand, expression and localization of tight junction proteins was induced by insulin-like growth factor I(IGF-I) in cultured rat submandibular gland cells, suggesting that IGF-I contributes to maintenance of the paracellular pathway system in salivary gland. Since the IGF-I effect was inhibited by PI3-kinase inhibitors, phosphorylation by PI3-kinase appears to contribute to the effect of IGF-I.

Research Products

• [Journal Article] The actin-specific reagent jasplakinolide induces apoptosis in primary rat parotid acinar cells (2012)
  • Author(s): Matsuki-Fukushima M, Hashimoto S, Murakami M, Ogata Y, Fujita-Yoshigaki J, Narita T, Sugiya H
  • Journal Title: Arch Oral Biol Volume: 57(5) Pages: 567-576
  • Peer Reviewed
• [Journal Article] Role of aquaporin-6 in rat parotid secretory granules (2011)
  • Author(s): Matsuki-Fukushima M, Fujita-Yoshigaki J, Murakami M, Katsumata-Kato O, Sugiya H
  • Journal Title: J Oral Biosci Volume: 53(4) Pages: 312-317
  • Peer Reviewed
• [Journal Article] 唾液分泌のメカニズム (2011)
  • Author(s): 杉谷博士
  • Journal Title: 日本口腔外科学会雑誌 Volume: 57(4) Pages: 182-186
• [Journal Article] 唾液腺機能とアクアポリン5 (2011)
  • Author(s): 杉谷博士
  • Journal Title: 日大口腔科学 Volume: 37(2) Pages: 75-82
• [Journal Article] Maintenance of paracellular barrier function by insulin-like growth factor-I in submandibular gland cells (2010)
  • Author(s): Mitsui R, Fujita-Yoshigaki J, Narita T, Matsuki-Fukushima M, Satoh K, Qi B, Guo MY, Katsumata-Kato O, Sugiya H
  • Journal Title: Arch Oral Biol Volume: 55(12) Pages: 963-969
  • Peer Reviewed
• [Journal Article] Characteristics of neurokinin A-induced salivary fluid secretion in perfused rat submandibular gland (2010)
  • Author(s): Qi B, Narita T, Satoh K, Guo MY, Katsumata-Kato O, Murakami M, Fujita-Yoshigaki J, Sugiya H
  • Journal Title: Arch Oral Biol Volume: 55(10) Pages: 737-44
  • Peer Reviewed
• [Journal Article] C2-ceramide inhibits the prostaglandin E2-induced accumulation of cAMP in human gingival fibroblasts (2010)
  • Author(s): Nakao S, Moriyama S, Segawa M, Guo MY, Sugiya H
  • Journal Title: Biomed Res Volume: 31(2) Pages: 97-103
  • Peer Reviewed
• [Journal Article] Thiol-oxidation reduces the release of amylase induced by β-adrenergic receptor activation in rat parotid acinar cells (2010)
  • Author(s): Guo M-Y, Satoh K, Qi B, Narita T, Katsumata-Kato O, Matsuki-Fukushima M, Fujita-Yoshigaki J, Sugiya H
  • Journal Title: Biomed Res Volume: 31(5) Pages: 293-299
  • Peer Reviewed
• [Journal Article] 唾液腺からの水とタンパク質の分泌の仕組み (2010)
  • Author(s): 杉谷博士
  • Journal Title: 歯科学報 Volume: 110(6) Pages: 800-805
• [Journal Article] E2F-1-deficient NOD/SCID mice developed showing decreased saliva production (2009)
  • Author(s): Matsui-Inohara H, Uematsu H, Narita T, Satoh K, Yonezawa H, Kuroda K, Ito T, Yoneda S, Kawarai T, Sugiya H, Watanabe H, Senpuku H
  • Journal Title: Exp Biol Med(Maywood) Volume: 234(12) Pages: 1525-36
  • Peer Reviewed
• [Journal Article] Role of protein kinase C in cAMP-dependent exocytosis in parotid acinar cells (2009)
  • Author(s): Sugiya H, Satoh K
  • Journal Title: Jap Dent Sci Rev Volume: 45 Pages: 121-126
  • Peer Reviewed
• [Journal Article] RP-HPLC-ESI-MS characterization of novel peptide fragments related to rat parotid secretory protein in parasympathetic induced saliva (2009)
  • Author(s): Ekstrom J, Murakami M, Inzitari R, Khosravani N, Fanali C, Cabras T, Fujita-Yoshigaki J, Sugiya H, Messana I, Castagnola M
  • Journal Title: J Sep Sci Volume: 32(17) Pages: 2944-52
  • Peer Reviewed
• [Journal Article] Separation of immature granules containing color dye from the rat parotid gland (2009)
  • Author(s): Katsumata-Kato O, Fujita-Yoshigaki J, Narita T, Sugiya H
  • Journal Title: J Med Invest Volume: 56(Suppl) Pages: 391-2
  • Peer Reviewed
• [Journal Article] Role of protein kinase C-delta in isoproterenol-induced amylase release in rat parotid acinar cells (2009)
  • Author(s): Sugiya H, Satoh K, Matsuki-Fukushima M, Qi B, Guo MY, Fujita-Yoshigaki J
  • Journal Title: J Med Invest Volume: 56(Suppl) Pages: 368-70
  • Peer Reviewed
• [Journal Article] Electrophysiological properties of AQP6 in mouse parotid acinar cells (2009)
  • Author(s): Ichikawa H, Shibukawa Y, Sahara Y, Tsumura M, Qi B, Satoh K, Narita T, Hashimoto S, Momose Y, Tazaki M, Shimono M, Sugiya H
  • Journal Title: J Med Invest Volume: 56(Suppl) Pages: 347-9
  • Peer Reviewed
• [Journal Article] The thiol-oxidizing agent diamide reduces isoproterenol-stimulated amylase release in rat parotid acinar cells (2009)
  • Author(s): Guo MY, Narita T, Qi B, Satoh K, Katsumata-Kato O, Matsuki-Fukushima M, Fujita-Yoshigaki J, Sugiya H
  • Journal Title: J Med Invest Volume: 56(Suppl) Pages: 284-6
  • Peer Reviewed
• [Journal Article] Pilocarpine-induced salivary fluid secretion in the perfused submandibular gland of the rat (2009)
  • Author(s): Qi B, Narita T, Sugiya H, Murakami M
  • Journal Title: J Med Invest Volume: 56(Suppl) Pages: 281-3
  • Peer Reviewed
• [Journal Article] Characterization of neurokinin A-evoked salivary secretion in the perfused rat submandibular gland (2009)
  • Author(s): Narita T, Qi B, Fukano M, Matsuki-Fukushima M, Murakami M, Sugiya H
  • Journal Title: J Med Invest Volume: 56(Suppl) Pages: 278-80
  • Peer Reviewed
• [Journal Article] E2F1-deficient NOD/SCID mice are an experimental model for dry mouth (2009)
  • Author(s): Satoh K, Narita T, Matsui-Inohara H, Ito T, Senpuku H, Sugiya H
  • Journal Title: J Med Invest Volume: 56(Suppl) Pages: 260-1
  • Peer Reviewed
• [Journal Article] Parotid acinar cells transiently change to duct-like cells during epithelial-mesenchymal transition (2009)
  • Author(s): Fujita-Yoshigaki J, Qi B, Narita T, Sugiya H
  • Journal Title: J Med Invest Volume: 56(Suppl) Pages: 258-9
  • Peer Reviewed
• [Presentation] ラット顎下腺に特異的なAQP5タンパク質修飾 (2011)
  • Author(s): 成田貴則, 岡林堅, 杉谷博士
  • Organizer: 第56回日本唾液腺学会
  • Place of Presentation: 東京
  • Year and Date: 2011-12-03
• [Presentation] 耳下腺腺房細胞の分泌顆粒形成におけるエンドサイトーシスの関与 (2011)
  • Author(s): 加藤治, 福島美和子, 成田貴則, 杉谷博士, 吉垣順子
  • Organizer: 第56回日本唾液腺学会
  • Place of Presentation: 東京
  • Year and Date: 2011-12-03
• [Presentation] ヒト歯髄培養細胞におけるエンドサイトーシスを介したMMP-3によるCTGF/CCN2発現調節 (2011)
  • Author(s): 室町幸一郎, 神尾直人, 成田貴則, 神尾素代, 杉谷博士, 松島潔
  • Organizer: 第135回日本歯科保存学会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-21
• [Presentation] NOD/SCID. E2f1-/-マウスにおける口腔乾燥症の病態解析 (2011)
  • Author(s): 佐藤慶太郎, 成田貴則, 福島美和子, 伊藤龍朗, 泉福英信, 杉谷博士
  • Organizer: 第53回歯科基礎医学会
  • Place of Presentation: 岐阜
  • Year and Date: 2011-10-01
• [Presentation] ラット顎下腺におけるAQP5タンパク質修飾 (2011)
  • Author(s): 成田貴則, 岡林堅, 杉谷博士
  • Organizer: 第152回日本獣医学会
  • Place of Presentation: 大阪
  • Year and Date: 2011-09-20
• [Presentation] エタクリン酸によるラット耳下腺腺房細胞の調節性アミラーゼ分泌阻害 (2011)
  • Author(s): 岡林堅, 高橋悠, 山田恵, 田川知嘉, 成田貴則, 杉谷博士
  • Organizer: 第152回日本獣医学会
  • Place of Presentation: 大阪
  • Year and Date: 2011-09-19
• [Presentation] 耳下腺cAMP依存性開口分泌におけるRhoA-Rhoキナーゼ系の関与 (2011)
  • Author(s): 杉谷博士, 成田貴則, 岡林堅
  • Organizer: 第152回日本獣医学会
  • Place of Presentation: 大阪
  • Year and Date: 2011-09-19
• [Presentation] アクアポリン5と6による分泌顆粒の浸透圧調節機構 (2011)
  • Author(s): 福島美和子, 吉垣純子, 勝俣治, 祁兵, 郭明宇, 成田貴則, 中尾寿美, 茂呂周, 浅野正岳, 伊藤芳久, 小菅康弘, 杉谷博士
  • Organizer: 第9回日本大学口腔科学会
  • Place of Presentation: 松戸
  • Year and Date: 2011-09-06
• [Presentation] ヒト歯髄培養細胞においてCTGF/CCN2はMMP-3により産生され細胞遊走および石灰化に関与する (2011)
  • Author(s): 室町幸一郎, 神尾直人, 成田貴則, 神尾素代, 細谷史規, 山浦賀弘, 三浦孝司, 杉谷博士, 松島潔
  • Organizer: 第134回日本歯科保存学会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-09
• [Presentation] マウス耳下腺腺房細胞におけるAQP6の電気生理学による特性 (2011)
  • Author(s): 市川秀樹, 澁川義幸, 佐原資謹, 津村麻記, 祁兵, 佐藤慶太郎, 成田貴則, 橋本貞充, 百瀬弥寿徳, 田崎雅和, 下野正基, 杉谷博士
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 盛岡
  • Year and Date: 2011-05-21
• [Presentation] ラット耳下腺分泌顆粒の浸透圧調節におけるアクアポリン6の寄与 (2011)
  • Author(s): 福島美和子, 杉谷博士, 村上政隆
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 盛岡
  • Year and Date: 2011-05-20
• [Presentation] 耳下腺細胞のアミラーゼ調節性分泌におけるPKA-PKCδ-MARCKS系シグナルの関与 (2011)
  • Author(s): 佐藤慶太郎, 杉谷博士
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 盛岡
  • Year and Date: 2011-05-20
• [Presentation] cAMP-dependent exocytosis in parotid acinar cells (2011)
  • Author(s): Sugiya H
  • Organizer: Gordon Research Conference 'Salivary Glands & Exocrine Biology'
  • Place of Presentation: Galveston, Texas, USA
  • Year and Date: 2011-02-10
• [Presentation] 口腔乾燥症病態モデルとしてのNOD/SCID. E2f1^<-/->マウス (2010)
  • Author(s): 成田貴則, 佐藤慶太郎, 福島美和子, 祁兵, 岡林堅, 泉福英信, 杉谷博士
  • Organizer: 第55回日本唾液腺学会
  • Place of Presentation: 東京
  • Year and Date: 2010-12-04
• [Presentation] Analysis of saliva fluid flow by perfusion system (2010)
  • Author(s): Qi B, Narita T, Murakami M, Sugiya H
  • Organizer: 88^<th> IADR
  • Place of Presentation: Balcerona, Spain
  • Year and Date: 2010-07-17
• [Presentation] Transient expression of claudin-4 in salivary glands indeuced by g-irradiation (2010)
  • Author(s): Fujita-Yoshigaki J, Narita T, Sugiya H
  • Organizer: 88^<th> IADR
  • Place of Presentation: Balcerona, Spain
  • Year and Date: 2010-07-15
• [Presentation] Involvement of aquaporin-6 in osmoregulation of rat parotid secretory granules (2010)
  • Author(s): Matsuki-Fukushima M, Murakami M, Fujita-Yoshigaki J, Katsumata-Kato O, Sugiya H
  • Organizer: 88^<th> IADR
  • Place of Presentation: Balcerona, Spain
  • Year and Date: 2010-07-15
• [Presentation] Study of dry mouth behavior of E2F-1-deficient NOD/SCID mice (2010)
  • Author(s): Satoh K, Narita T, Matsui-Inohara H, Ito T, Senpuku H, Sugiya H
  • Organizer: 88^<th> IADR
  • Place of Presentation: Balcerona, Spain
  • Year and Date: 2010-07-15
• [Presentation] 唾液と口腔疾-患唾液分泌の研究から (2010)
  • Author(s): 杉谷博士
  • Organizer: 第43回無菌生物ノートバイオロジー学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-01-21
• [Presentation] Separation of immature granules containing color dye from the rat parotid gland (2009)
  • Author(s): Katsumata-Kato O, Fujita-Yoshigaki J, Narita T, Sugiya H
  • Organizer: The 11^<th> International Symposium on Exocrine Secretion
  • Place of Presentation: Tokushima, Japan
  • Year and Date: 2009-07-25
• [Presentation] Role of protein kinase C in cAMP-dependent exocytosis in parotid acinar cells (2009)
  • Author(s): Sugiya H, Satoh K, Matsuki-Fukushima M, Qi B, Guo MY, Fujita-Yoshigaki J
  • Organizer: The 11^<th> International Symposium on Exocrine Secretion
  • Place of Presentation: Tokushima, Japan
  • Year and Date: 2009-07-25
• [Presentation] Electrophysiological properties of AQP6 in mouse parotid acinar cells (2009)
  • Author(s): Ichikawa H, Shibukawa Y, Sahara Y, Tsumura M, Qi B, Satoh K, Narita T, Hashimoto S, Momose Y, Tazaki M, Shimono M, Sugiya H
  • Organizer: The 11^<th> International Symposium on Exocrine Secretion
  • Place of Presentation: Tokushima, Japan
  • Year and Date: 2009-07-25
• [Presentation] The thiol-oxidizing agent diamide reduces isoproterenol-stimulated amylase release in rat parotid acinar cells (2009)
  • Author(s): Guo MY, Narita T, Qi B, Satoh K, Katsumata-Kato O, Matsuki-Fukushima M, Fujita-Yoshigaki J, Sugiya H
  • Organizer: The 11^<th> International Symposium on
  • Place of Presentation: Tokushima, Japan
  • Year and Date: 2009-07-25
• [Presentation] Pilocarpine-induced salivary fluid secretion in the perfused submandibular gland of the rat (2009)
  • Author(s): Qi B, Narita T, Sugiya H, Murakami M
  • Organizer: The 11^<th> International Symposium on
  • Place of Presentation: Tokushima, Japan
  • Year and Date: 2009-07-25
• [Presentation] Characterization of neurokinin A-evoked salivary secretion in the perfused rat submandibular gland (2009)
  • Author(s): Narita T, Qi B, Fukano M, Matsuki-Fukushima M, Murakami M, Sugiya H
  • Organizer: The 11^<th> International Symposium on
  • Place of Presentation: Tokushima, Japan
  • Year and Date: 2009-07-25