Role of SPARC on malignant phenotype of oral squamous cell carcinoma

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592403
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Ohu University (2010-2011); Kanagawa Dental College (2009)
• Principal Investigator: KATO Yasumasa 奥羽大学, 歯学部, 教授 (50214408)
• Co-Investigator(Renkei-kenkyūsha): OZAWA Shigeyuki 神奈川歯科大学, 歯学部, 助教 (40434394)
• Project Period (FY): 2009 – 2011
• Keywords: SPARC / 癌 / 肺転移

Research Abstract

Here we established new SPARC knock out mouse on C3H strain from MF strain by back crossing method. Using microarray technique, expression of 6 kinds of gene such as Camp, Ngp, Ig-., Saa3, Gpr84, Ptk6 was induced in the lung of SPARC knock our mice. These were suggested to be candidate genes associating lung metastasis of cancer.

Research Products

• [Journal Article] 学校法人神奈川歯科大学の学生・職員における喫煙経験と性・年齢,学生の所属,職員の職種,健康状態,および喫煙に対する知識・意識との関係 (2011)
  • Author(s): 山本龍生, 加藤靖正, 菅谷彰, 塚本秀人, 辻本勝美, 中村澄夫, 林田丞太, 町田宏夫, 平田幸夫
  • Journal Title: 神奈川歯学 Volume: 46 Pages: 57-64
  • Peer Reviewed
• [Journal Article] Hemangioma with phleboliths in the floor of the mouth presenting as a submental swelling : a case report (2011)
  • Author(s): Baba Y, Kato Y
  • Journal Title: J. Med. Case Reports Volume: 2(1) Pages: 28-30
  • DOI: DOI:10.4021/jmc108w
  • Peer Reviewed
• [Journal Article] Unusual computed tomography findings of radio-necrosis after chemoradiation of stage IV hypopharyngeal cancer : a case report (2011)
  • Author(s): Baba Y, Kato Y, Ogawa K
  • Journal Title: J. Med. Case Reports Volume: 5(1) Pages: 25
  • DOI: DOI:10.1186/1752-1947-5-25
  • Peer Reviewed
• [Journal Article] Hyperplasia of lymphoid structures in the hypopharynx : a case report (2010)
  • Author(s): Baba Y, Kato Y, Ogawa K
  • Journal Title: J. Med. Case Reports Volume: 4 Pages: 388
  • DOI: DOI:10.1186/1752-1947-4-388
  • Peer Reviewed
• [Journal Article] Heterozygous mutation(G/G→G/A) at 2607 is closely associated with increase in EGFR copy number and mRNA half life but impaired EGFR protein synthesis in squamous cell carcinoma of the head and neck-implication to the gefitinib efficacy- (2010)
  • Author(s): Nakazaki K, Kato Y, Taguchi T, Inayama Y, Ishiguro Y, Tsukuda M
  • Journal Title: Oncol. Lett Volume: 1 Pages: 1017-1020
  • DOI: DOI:10.3892/ol.2010.188
  • Peer Reviewed
• [Journal Article] Reactive oxygen species(ROS) reduce the expression of BRAK/CXCL14 in human head and neck squamous cell carcinoma cells (2010)
  • Author(s): Maehata Y, Ozawa S, Kobayashi K, Kato Y, Yoshino F, Miyamoto C, Izukuri K, Kubota, E, Hata R, Lee MC
  • Journal Title: Free Radical Res Volume: 44(8) Pages: 913-24
  • DOI: DOI:10.3109/10715762.2010.490836
  • Peer Reviewed
• [Journal Article] MAP kinase mediates UV irradiation induced up-regulation of the gene expression of chemokine BRAK/CXCL14 (2010)
  • Author(s): Ozawa S, Ito S, Kato Y, Kubota E, Hata R.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 396(4) Pages: 1060-1064
  • DOI: DOI:10.1016/j.bbrc.2010.05.072
  • Peer Reviewed
• [Journal Article] Functional characterization of proximal promoter of gene for human chemokine BRAK/CXCL14 (2010)
  • Author(s): Komori R, Ozawa S, Kato Y, Shinji H, Kimoto S, Hata R
  • Journal Title: Biomed. Res Volume: 31(2) Pages: 123-131
  • DOI: DOI:10.2220/biomedres.31.123
  • Peer Reviewed
• [Journal Article] Expression of tumor-suppressing chemokine BRAK/CXCL14 reduces cell migration rate of HSC-3 tongue carcinoma cells and stimulates attachment to collagen and formation of elongated focal adhesions in vitro (2010)
  • Author(s): Sato K, Ozawa S, Izukuri K, Kato Y, Hata R
  • Journal Title: Cell Biol. Int Volume: 34 Pages: 513-522
  • DOI: DOI:10.1042/CBI20090108
  • Peer Reviewed
• [Journal Article] Inostamycin prevents malignant phenotype of cancer : inhibition of phosphatidyl-inositol synthesis provides a therapeutic advantage for head and neck squamous cell carcinoma (2010)
  • Author(s): Baba Y, Kato Y, Ogawa K
  • Journal Title: Cell Biol Int Volume: 34(2) Pages: 171-175
  • DOI: DOI:10.1042/CBI20090310
  • Peer Reviewed
• [Journal Article] BRAK/CXCL14 expression in oral carcinoma cells completely suppresses tumor cell xenografts in SCID mouse (2009)
  • Author(s): Ozawa S, Kato Y, Kubota E, Hata R
  • Journal Title: Biomed. Res Volume: 30(5) Pages: 315-318
  • DOI: DOI:10.2220/biomedres.30.315
  • Peer Reviewed
• [Journal Article] Restoration of BRAK/CXCL14 gene expression by gefitinib is associated with antitumor efficacy of the drug in head and neck squamous cell carcinoma (2009)
  • Author(s): Ozawa S, Kato Y, Ito S, Komori R, Shiiki N, Tsukinoki K, Ozono S, Maehata Y, Taguchi T, Imagawa-Ishiguro Y, Tsukuda M, Kubota E, Hata R
  • Journal Title: Cancer Sci Volume: 100(11) Pages: 2202-2209
  • DOI: DOI:10.1111/j.1349-7006.2009.01281.x
  • Peer Reviewed
• [Journal Article] Management of deep neck infection by a transnasal approach : a case report (2009)
  • Author(s): Baba Y, Kato Y, Saito H, Ogawa, K
  • Journal Title: J. Med. Case Reports Volume: 3 Pages: 7317
  • DOI: DOI:10.4076/1752-1947-3-7317
  • Peer Reviewed
• [Presentation] TRPM5は、酸性細胞外pHシグナルに関与し、メラノーマの肺転移に関与する (2011)
  • Author(s): 加藤靖正, 畑隆一郎, 佃守, 宮崎香
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 20111000
• [Presentation] オステオネクチンノックアウトマウスの肺における遺伝子発現プロファイリング (2011)
  • Author(s): 加藤靖正, 前畑洋次郎, 前田豊信, 畑隆一郎
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岐阜
  • Year and Date: 20111000
• [Presentation] Cell-dependent suppression of tumor growth and metastasis in CXCK14/BRAK transgenic mice (2011)
  • Author(s): Hata R, Izukuri K, Kato Y. NK
  • Organizer: Roche/Nature Medicine symposium on cancer immunology and immunotherapy
  • Place of Presentation: Nutley, NJ, USA
  • Year and Date: 20110911-13
• [Presentation] ケモカインCXCL14/BRAKは幹細胞分化促進因子か (2011)
  • Author(s): 畑隆一郎, 佐藤-楠畑かおり, 小澤重幸, 加藤靖正, 古江-楠田美保
  • Organizer: 第43回日本結合組織学会学術大会/第58回マトリックス研究会大会合同学術集会
  • Place of Presentation: 別府
  • Year and Date: 20110600
• [Presentation] Analysis of promoter region of human BRAK/CXCL14 in SCC cells (2011)
  • Author(s): Komori R, Ozawa S, Kato Y, Maehata Y, Shinji H, Hata R, Kimoto S
  • Organizer: 89th General Session & Exhibition of the IADR/40th Annual Meeting of the AADR/35th Annual Meeting of the CADR
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 20110314-10
• [Presentation] CXCL14 transgenic mice are resistant to carcinoma growth and metastasis (2011)
  • Author(s): Hata R, Izukuri K, Kato Y
  • Organizer: 89th General Session & Exhibition of the IADR/40th Annual Meeting of the AADR/35th Annual Meeting of the CADR
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 20110314-10
• [Presentation] 習慣的喫煙経験と学生の学科,職員の職種,健康状態,喫煙の知識・意識との関係 (2010)
  • Author(s): 山本龍生, 加藤靖正, 菅谷彰, 菅原光則, 田口利秋, 塚本秀人, 辻本勝美, 長尾健, 中村澄夫, 林弘之, 林田丞太, 町田宏夫, 米田睦夫, 平田幸夫
  • Organizer: 神奈川歯科大学学会第45回総会
  • Place of Presentation: 横須賀
  • Year and Date: 20101200
• [Presentation] ROCK阻害剤Fasudilによる抗腫瘍性ケモカインBRAK/CXCL14の細胞外分泌促進と腫瘍進展抑制の検討 (2010)
  • Author(s): 宮本千央, 前畑洋次郎, 小澤重幸, 生駒丈晴, 小森令賀, 居作和人, 加藤靖正, 畑隆一郎, 李昌一
  • Organizer: 第123回日本薬理学会関東部会
  • Place of Presentation: 栃木県下野市
  • Year and Date: 20101000
• [Presentation] 癌進展抑制因子BRAK/CXCL14遺伝子の発現制御機構-MAPキナーゼシグナルのクロストークによる制御- (2010)
  • Author(s): 畑隆一郎, 居作和人, 加藤靖正
  • Organizer: 第52回歯科基礎医学会学術大会・総会
  • Place of Presentation: 東京
  • Year and Date: 20100900
• [Presentation] BRAK/CXCL14遺伝子トランスジェニックマウスの腫瘍抑制・転移抑制機構の解明 (2010)
  • Author(s): 居作和人, 加藤靖正, 畑隆一郎
  • Organizer: 第52回歯科基礎医学会学術大会・総会
  • Place of Presentation: 東京
  • Year and Date: 20100900
• [Presentation] 阻害剤はケモカインBRAK/CXCL14の細胞外分泌を促進し抗腫瘍効果を発揮する (2010)
  • Author(s): 宮本千央, 前畑洋次郎, 高橋俊介, 高橋聡子, 杉山秀太, 徳富文彬, 加藤靖正, 畑隆一郎, 李昌一
  • Organizer: 第52回歯科基礎医学会学術大会・総会
  • Place of Presentation: 東京
  • Year and Date: 20100900
• [Presentation] ROCK阻害剤FasudilはケモカインBRAK/CXCL14の細胞外分泌を促進し抗腫瘍効果を発揮する (2010)
  • Author(s): 宮本千央, 前畑洋次郎, 小澤重幸, 加藤靖正, 畑隆一郎, 李昌一
  • Organizer: 第42回日本結合組織学会学術大会/第57回マトリックス研究会大会合同学術集会
  • Place of Presentation: 秋田
  • Year and Date: 20100800
• [Presentation] ST2細胞においてSimvastatinは骨分化を促進し、脂肪分化を抑制する (2010)
  • Author(s): 前田豊信, 加藤靖正, 堀内登
  • Organizer: 第42回日本結合組織学会学術大会/第57回マトリックス研究会大会合同学術集会
  • Place of Presentation: 秋田
  • Year and Date: 20100800
• [Presentation] 口腔扁平上皮癌細胞においてGefitinib(Iressa)は酸化ストレスに誘導される抗腫瘍性ケモカインBRAK/CXCL14の発現低下を抑制する (2010)
  • Author(s): 前畑洋次郎, 小澤重幸, 小林杏, 吉野文彦, 宮本千央, 加藤靖正, 畑隆一郎, 李昌一
  • Organizer: 第123回日本薬理学会関東部会
  • Place of Presentation: 栃木県下野市
  • Year and Date: 2010-10-23
• [Presentation] 頭頸扁平上皮癌において, GefitinibはBRAK/CXCL14の発現を介して抗腫瘍効果を示す (2009)
  • Author(s): 加藤靖正, 田口享秀, 石黒由香利, 佃守
  • Organizer: 第22回日本バイオセラピィ学会学術集会総会
  • Place of Presentation: 大阪
  • Year and Date: 20091100
• [Presentation] 頭頸部癌進展抑制因子BRAK/CXCL14遺伝子の発現制御配列の解析 (2009)
  • Author(s): 畑隆一郎, 加藤靖正
  • Organizer: 第51回歯科基礎医学会学術大会・総会
  • Place of Presentation: 新潟
  • Year and Date: 20090900
• [Book] 口腔生化学題5版 (2011)
  • Author(s): 畑隆一郎、高橋信博、宇田川信之、東俊文、上條竜太郎、石崎明、加藤靖正、早川太郎、須田立雄、木崎治俊
  • Publisher: 医歯薬出版