A basic research to establish molecular targeted therapy for odontogenic cysts

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592433
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Nihon University
• Principal Investigator: YAMAGUCHI Yoko 日本大学, 歯学部, 助手 (00239922)
• Co-Investigator(Kenkyū-buntansha): OHSHIMA Mitsuhiro 奥羽大学, 薬学部, 教授 (30194145)
• Co-Investigator(Renkei-kenkyūsha): ABIKO Yoshimitsu 日本大学, 松戸歯学部, 教授 (70050086)
• Project Period (FY): 2009 – 2011
• Keywords: 歯原性嚢胞

Research Abstract

To establish molecular targeted therapy for odontogenic cysts, a basic in vitro study was carried out. Fibroblasts originated from apical cyst and keratocystic odontogenic tumor were treated with various growth factor receptor kinase inhibitors, and the survival level of the cells was investigated. Since PD166866 and PD089828 induced effective fibroblast cell death, these receptor tyrosine kinase inhibitors may be candidate drugs for molecular targeted, non-surgical therapy for odontogenic cysts.

Research Products

• [Journal Article] TGF-beta Signaling in Gingival Fibroblast-Epithelial Interaction (2010)
  • Author(s): M. Ohshima, Y. Yamaguchi, N. Matsumoto, P. Micke, Y. Takenouchi, T. Nishida, M. Kato, K. Komiyama, Y. Abiko, K. Ito, K. Otsuka, K. Kappert
  • Journal Title: J Dent Res Volume: 89 Pages: 1315-1321
  • Peer Reviewed
• [Journal Article] Insulin-like growth factor binding proteins(IGFBP)-2,-3 in gingival crevicular fluid (2010)
  • Author(s): Takenouchi Y, Ohshima M, Yamaguchi Y, Nishida T, Senda N, Idesawa M, Otsuka K, Ito K
  • Journal Title: J Periodontal Res Volume: 45 Pages: 803-808
  • Peer Reviewed
• [Presentation] 受容体チロシンキナーゼ阻害薬による非外科的顎.胞治療法の開発 (2011)
  • Author(s): 大島光宏、山口洋子、石井輝彦、大木秀郎
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会議場、京都
  • Year and Date: 2011-09-23
• [Presentation] FLT-1 represents a potential therapeutic target in human periodontitis. Gordon Research Conference in Periodontal Diseases (2011)
  • Author(s): Mitsuhiro Ohshima, Yoko Yamaguchi, Yoshimitsu Abiko
  • Organizer: Davidson College
  • Place of Presentation: Davidson, NC, USA
  • Year and Date: 2011-07-21
• [Presentation] 生体外歯周炎モデルによる生薬のスクリーニング (2010)
  • Author(s): 山口洋子、鳥居塚和生、大島光宏
  • Organizer: 第33回分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際会議場、兵庫
  • Year and Date: 2010-12-10
• [Presentation] 生薬による生体外歯周炎モデルのコラーゲン分解阻害薬 (2010)
  • Author(s): 山口洋子、鳥居塚和生、大島光宏
  • Organizer: 日本薬学会東北支部
  • Place of Presentation: 奥羽大学、福島
  • Year and Date: 2010-10-24
• [Presentation] 3次元培養法を用いた新規歯周炎治療薬のスクリーニング (2010)
  • Author(s): 山口洋子、大島光宏
  • Organizer: 第42回日本結合組織学会学術大会・第57回マトリックス研究会大会合同学術集会
  • Place of Presentation: 秋田拠点セン拠点センター、秋田
  • Year and Date: 2010-08-19
• [Presentation] TGF-beta signaling in aggressive matrix destruction by gingival fibroblasts (2009)
  • Author(s): Mitsuhiro Ohshima, Yoko Yamaguchi, Naoyuki Matsumoto, Patrick Micke, Yuka Takenouchi, Tetsuya Nishida, Mitsuyasu Kato, Kazuo Komiyama, Yoshimitsu Abiko, Koichi Ito, Kichibee Otsuka, Kai Kappert
  • Organizer: TGF-beta meeting in Uppsala
  • Place of Presentation: Uppsala, Sweden
  • Year and Date: 2009-08-27
• [Presentation] A novel drug screening system for periodontitis using three-dimensional culture (2009)
  • Author(s): Mitsuhiro Ohshima, Yoko Yamaguchi, Naoyuki Matsumoto, Yuka Takenouchi, Tetsuya Nishida, Mitsuyasu Kato, Kazuo Komiyama, Yoshimitsu Abiko, Koichi Ito, Kichibee Otsuka
  • Organizer: Gordon Research Conference in Periodontal Diseases, Colby-Sawyer College
  • Place of Presentation: New London, NH, USA
  • Year and Date: 2009-08-04
• [Presentation] A novel drug screening system for periodontitis using 3D culture (2009)
  • Author(s): Mitsuhiro Ohshima, Yoko Yamaguchi, Naoyuki Matsumoto, Yuka Takenouchi, Tetsuya Nishida, Mitsuyasu Kato, Kazuo Komiyama, Yoshimitsu Abiko, Koichi Ito, Kichibee Otsuka
  • Organizer: Europerio 6
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2009-06-05
• [Patent(Industrial Property Rights)] 口腔癌細胞浸潤阻害剤 (2011)
  • Inventor(s): 山口洋子、大島光宏、鳥居塚和生
  • Industrial Property Rights Holder: 日本大学,昭和大学
  • Industrial Property Number: 特許権、特願2011-220423
  • Filing Date: 2011-10-04
• [Patent(Industrial Property Rights)] 顎.胞治療薬 (2010)
  • Inventor(s): 山口洋子、大島光宏、大木秀郎
  • Industrial Property Rights Holder: 日本大学
  • Patent Publication Number: 特許権、特開2012-012365
  • Filing Date: 2010-07-05
• [Patent(Industrial Property Rights)] コラーゲン分解阻害剤 (2010)
  • Inventor(s): 山口洋子、大島光宏、鳥居塚和生
  • Industrial Property Rights Holder: 日本大学,昭和大学
  • Patent Publication Number: 特許権、特開2011-256136
  • Filing Date: 2010-06-09