2011 Fiscal Year Final Research Report
Immune-histological observations of reconstructed gingiva-like cultures using epithelial cells derived from PDL
| Project/Area Number |
21592434
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
IGARASHI Masaru 日本歯科大学, 新潟生命歯学部, 教授 (90168104)
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| Co-Investigator(Kenkyū-buntansha) |
KITAJIMA Kayoko 日本歯科大学, 新潟生命歯学部, 准教授 (00177841)
ARAI Kyoko 日本歯科大学, 新潟生命歯学部, 講師 (10434143)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 歯根膜 / 上皮細胞 / 線維芽細胞 / 再構成培養 / 免疫染色 / コラーゲンゲル / 分化 / 移植 |
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| Research Abstract |
This investigation showed the comparison the differentiations and proliferations of the epithelial cells derived from periodontal ligaments or Hertwig's epithelial root sheath against to gingiva. The three dimensional organotypic cultures were incubated under air-liquid interface environment. The specimens showed that the multilayered epithelial structures without keratinization and the dispersing tendency of the cells in the epithelial layer were seen after 2 weeks incubation. The results showed that the reconstructed tissues were not similar to original gingiva. The continuous proper growth without scar after transplantation to immunodeficiency mice indicated the usefulness of transplantations.
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