Mesenchymal stem cells-photocurable gelatin-based gelation material for application to periodontal regeneration

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592501
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Keio University
• Principal Investigator: NAKAGAWA Taneaki 慶應義塾大学, 医学部, 教授 (00227745)
• Co-Investigator(Kenkyū-buntansha): MORIKAWA Satoru 慶應義塾大学, 医学部, 助教 (00424169) ; HOSAKA Yasuo 慶應義塾大学, 医学部, 助教 (30246350)
• Project Period (FY): 2009 – 2011
• Keywords: 再生歯学

Research Abstract

We confirmed that prospective isolatedMSCs showed a 120, 000-fold higher colony forming frequency thanthe conventional MSCs. Furthermore, Our results suggested that MSCs in adultbone marrow have at least two developmental origins, one of which is the neural crest. Furthermore, prospectively enrichedMSCs are a promising candidate for the efficient generation of high-quality iPS cells. This photocurable solution also has adequate viscosity at room temperature before curing, and it manipulation is enhanced. It is assumed that forms suitable for various bone defects will thus be easily rendered, and it is also assumed that the shape can be retained with the mechanical strength required by photocurable.

Research Products

• [Journal Article] Antibacterial activity of povidone. iodine against an artificial biofilm of Porphyromonas gingivalis and Fusobacterium nucleatum (2011)
  • Author(s): Yasuo Hosaka, Atsushi Saito, Ryo Maeda, Chie Fukaya, Satoru Morikawa, Asako Makino, Kazuyuki Ishihara, Taneaki Nakagawa
  • Journal Title: Archives of Oral Biology Volume: 57(4) Pages: 364-368
  • Peer Reviewed
• [Journal Article] Purified Mesenchymal Stem Cells Are an Efficient Source for iPS Cell Induction (2011)
  • Author(s): Kunimichi Niibe, Yoshimi Kawamura, Daisuke Araki, Satoru Morikawa, Kyoko Miura, Sadafumi Suzuki, Shigeto Shimmura, Takehiko Sunabori, Yo Mabuchi, Yasuo Nagai, Taneaki Nakagawa, Hideyuki Okano Yumi Matsuzaki.
  • Journal Title: PLoS ONE Volume: 6(3) Pages: e17610
  • Peer Reviewed
• [Journal Article] Mesp1+early paraxial mesodermal cells supply initial bone marrow mesenchymal stem cells capable of differentiating into neural crest lineage cells (2011)
  • Author(s): Kunimichi Niibe, Satoru Morikawa, Yo Mabuchi, Taneaki Nakagawa, Hideyuki Okano, Yumi Matsuzaki
  • Journal Title: Inflammation and Regeneration Jan Volume: 31(1) Pages: 116-124
  • Peer Reviewed
• [Journal Article] Anti-IL-6-receptor antibody promotes repair of spinal cord injury by inducing microglia-dominant inflammation (2010)
  • Author(s): Masahiko Mukaino, Masaya Nakamura, Osamu Yamada, Seiji Okada, Satoru Morikawa, Francois Renault-Mihara, Akio Iwanami, Takeshi Ikegami, Yoshiyuki Ohsugi, Osahiko Tsuji, Hiroyuki Katoh, Yumi Matsuzaki, Yoshiaki Toyama, Meigen Liu, and Hideyuki Okano
  • Journal Title: Experimental Neurology Aug Volume: 224(2) Pages: 403-414
  • Peer Reviewed
• [Journal Article] Inhibition of Abcg2 transporter on primitive hematopoietic stem cells by All-trans retinoic acid increases sensitivity to anthracycline (2010)
  • Author(s): Lawrence Lein, Yasuo Nagai, Yo Mabuchi, Sadafumi Suzuki, Satoru Morikawa, and Yumi Matsuzaki
  • Journal Title: Inflammation and Regeneration Jan Volume: 30(2) Pages: 55-62
  • Peer Reviewed
• [Journal Article] Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow (2009)
  • Author(s): Satoru Morikawa, Yo Mabuchi, Yoshiaki Kubota, Yasuo Nagai, Kunimichi Niibe, Emi Hiratsu, Sadafumi Suzuki, Chikako Miyauchi-Hara, Narihito Nagoshi, Takehiko Sunabori, Shigeto Shimmura, Atsushi Miyawaki, Taneaki Nakagawa, Toshio Suda, Hideyuki Okano, and Yumi Matsuzaki
  • Journal Title: The Journal of Experimental Medicine Volume: 206(11) Pages: 2483-2496
  • Peer Reviewed
• [Journal Article] Improvement of Hydrogelation Abilities and Handling of Photocurable Gelatin-based Crosslinking Materials (2009)
  • Author(s): Chie Fukaya, Yasuhide Nakayama, Yoshinobu Murayama, Sadao Omata, Ayaka Ishikawa, Yasuo Hosaka, Taneaki Nakagawa
  • Journal Title: Journal of Biomedical Materials Research Volume: 91(1) Pages: 329-36
  • Peer Reviewed
• [Presentation] ヒト間葉系幹細胞の予期的分離とClonal解析 (2010)
  • Author(s): 森川暁, 新部邦透, 深谷千絵, 穂坂康朗, 中川種昭
  • Organizer: 第53回日本歯周病学会春季学術大会
  • Place of Presentation: 盛岡
  • Year and Date: 20100514-15
• [Presentation] Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow (2010)
  • Author(s): Satoru Morikawa, Yo Mabuchi, Yoshiaki Kubota, Kunimichi Niibe, Narihito Nagoshi, Shigeto Shimmura, Taneaki Nakagawa, Toshio Suda, Hideyuki Okano, and Yumi Matsuzaki
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 20100318-19
• [Presentation] Flow cytometric isolation and clonal identification of mesenchymal stem cells in human bone marrow. (2009)
  • Author(s): Yo Mabuchi, Satoru Morikawa, Hideyuki Okano, Yumi Matsuzaki
  • Organizer: International Society for Stem Cell Research(ISSCR), 7^<th> ANNUAL MEETING
  • Place of Presentation: Barcelona(ポスター)
  • Year and Date: 20090708-11
• [Presentation] 間葉系幹細胞の一部は神経堤から発生する (2009)
  • Author(s): 森川暁, 深谷千絵, 穂坂康朗, 中川種昭
  • Organizer: 第52回日本歯周病学会春季学術大会
  • Place of Presentation: 岡山
  • Year and Date: 20090515-16
• [Presentation] Isolation and Identification of Mesenchymal Stem Cells in Human Bone Marrow (2009)
  • Author(s): 馬渕洋、森川暁、岡野栄之、松崎有未
  • Organizer: 第7回幹細胞シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 20090515-16
• [Presentation] Prospective Isolation of Mesenchymal Stem Cells (2009)
  • Author(s): S. MORIKAWA, C. FUKAYA, and T. NAKAGAWA
  • Organizer: 87^<th> General Session and Exhibition of the IADR(International Association for Dental Reseach)
  • Place of Presentation: Miami, Flolida, USA
  • Year and Date: 20090401-04