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2011 Fiscal Year Final Research Report

Epithelialization in oral mucous wound healing in terms of energy metabolism

Research Project

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Project/Area Number 21592512
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionGunma University

Principal Investigator

YOKOO Satoshi  群馬大学, 大学院・医学系研究科, 教授 (00322206)

Project Period (FY) 2009 – 2011
Keywordsケラチノサイト / 口腔粘膜 / 創傷治癒 / 脂肪酸 / パルミチン酸
Research Abstract

The purpose of this study was to clarify the healing capacity of wounds of the oral mucosa in comparison to those of the skin, and to evaluate the wound healing mechanism of oral mucosa using a cytobiological approach from the aspect of energy metabolism in oral keratinocytes. Samples of epidermal and oral keratinocytes collected at surgery and of cultured oral keratinocytes were used to analuze (1) by gas chromatography the composition of fatty acids (16:0, 18:2, 20:4) in the cell membranes of keratinocytes, (2) by immunohistochemical staining of GLUT-1 antibody and specific PAS staining the localization of glucose metabolism, and (3) by RT-PCR and Western blotting the expression of GLUT-1 mRNA and of protein in the keratinocytes of the basal and parabasal layers of each epithelial tissue. The % composition of palmitic acid (16:0) was significantly higher in buccal mucosal keratinocyte and in the gingival than in the epidermis. Immunohistochemical staining showed GLUT-1 protein in the skin to be expressed only in the bulge region of hair follicles and in the epidermal basal layer, and observed nearly throughout all epithelial cell layers in the oral mucosa. PAS-positive cell were observed among differentiation-enhanced cells in the upper prickle layer in oral mucosa. The same results were obtained from RT-RCR and a Western blotting analysis. The present study demonstrated definite cytobiological evidence that the oral mucosa surpasses the skin in regard to its wound healing capacity.

  • Research Products

    (2 results)

All 2011 2009

All Journal Article (2 results) (of which Peer Reviewed: 2 results)

  • [Journal Article] Cell Celluler fatty acid composition of stratified squamous epithelia after transplantation of ex vivo produced oral mucosa equivalent2011

    • Author(s)
      Moriyama K, Yokoo S, Terashi H, Komori T
    • Journal Title

      Kobe J Med Sci

      Volume: 56 Pages: 253-62

    • Peer Reviewed
  • [Journal Article] Epithelialization in oral mucosal wound healing in terms of energy metabolism2009

    • Author(s)
      Kuroki S, Yokoo S, Terashi H, Hasegawa M, Komori T
    • Journal Title

      Kobe J Med Sci

      Volume: 55 Pages: 5-15

    • Peer Reviewed

URL: 

Published: 2013-07-31  

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