Heparanase localization and stimulatory effect during formation and regeneration of periodontal tissues

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592571
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kyoto Prefectural University (2011); Osaka University (2009-2010)
• Principal Investigator: HIRATA Azumi 京都府立大学, 大学院・生命環境科学研究科, 助教 (40263587)
• Co-Investigator(Kenkyū-buntansha): UENO Takaaki 大阪医科大学, 口腔外科学教室, 教授 (60252996) ; TSUJI Takehito 岡山大学, 大学院・自然科学研究科, 准教授 (90314682) ; MATSUMURA Tatsushi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (70432648)
• Co-Investigator(Renkei-kenkyūsha): SUGAHARA Toshio 愛知学院大学, 歯学部, 客員教授 (10116048)
• Project Period (FY): 2009 – 2011
• Keywords: ヘパラナーゼ / 歯周組織 / 基底膜 / 器官培養 / モデルマウス / 幹細胞 / 組織再生

Research Abstract

We have previously reported that heparanase may contribute to root formation, especially cementoblast differentiation, by degrading perlecan in the dental basement membrane. The aim of this study was to determine whether heparanase might function in periodontal development and regeneration. Our studies indicated that 1) Heparanase localization was observed in osteoblasts and in the basal cells of the oral epithelium. 2) Palatal epithelial cells were labeled with intense heparanase signal during palatogenesis ; in contrast, perlecan labeling was sparse in the basement membrane of medial epithelial seam. 3) Exogenous heparanase might exhibit proliferative potential to dental mesenchymal stem cells in the apical space. These findings suggested that heparanase may have an important role during the process of formation and regeneration of periodontal tissues.

Research Products

• [Journal Article] Pulpal progenitors and dentin repair (2011)
  • Author(s): Harichane, Y. Hirata, A. Dimitrova-Nakov, S. Granja, I. Goldberg, M. Kellermann, O. and Poliard, A.
  • Journal Title: Advances in Dental Research Volume: 23 Pages: 307-312
  • DOI: DOI:10.1177/0022034511405322
  • Peer Reviewed
• [Journal Article] Localization of the HOXC homeobox gene family during palate formation in mice (2010)
  • Author(s): Hirata, A. Tsuji, T. Imura, H. Yamada, T. Ueno, T. Mishima, K. Natsume, N. Sugahara, T. Koga, Y. Takano, K. and Nakamura, H.
  • Journal Title: Selected papers from the XX Congress of the European Association of Cranio-Maxillo-Facial Surgery Pages: 415-420
• [Journal Article] Localization of Runx2, osterix and osteopontin in tooth root formation in rat molars (2009)
  • Author(s): Hirata, A. Sugahara, T. and Nakamura, H.
  • Journal Title: Journal of Histochemistry and Cytochemistry Volume: 57 Pages: 397-403
  • DOI: DOI:10.1369/jhc.2008.952192
  • Peer Reviewed
• [Presentation] Homeobox family HOX遺伝子は口蓋形成に関与する (2011)
  • Author(s): 平田あずみ、井村英人、南克浩、夏目長門、菅原利夫
  • Organizer: 第56回日本口腔外科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-21
• [Presentation] Homeobox family HOXC遺伝子は口蓋形成に関与する (2010)
  • Author(s): 平田あずみ、井村英人、山田朋弘、植野高章、三島克章、南克浩、夏目長門、菅原利夫
  • Organizer: 第55回日本口腔外科学会総会
  • Place of Presentation: 千葉
  • Year and Date: 2010-10-16
• [Presentation] Localization of the HOXC homeobox gene family during palate formation in mice (2010)
  • Author(s): Hirata, A. Tsuji, T. Imura, H. Yamada, T. Ueno, T. Mishima, K. Natsume, N. Sugahara, T. Koga, Y. Takano, K. and Nakamura, H.
  • Organizer: XX Congress of the European Association of Cranio-Maxillo-Facial Surgery
  • Place of Presentation: Bruges, Belgium
  • Year and Date: 2010-09-17
• [Presentation] 口蓋の骨形成過程におけるヘパラン硫酸とヘパラナーゼの役割 (2009)
  • Author(s): 平田あずみ、山田朋弘、植野高章、井村英人、香川智正、山近英樹、松村達志、森谷徳文、三島克章、菅原利夫
  • Organizer: 第54回日本口腔外科学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2009-10-10
• [Presentation] 口蓋の骨形成過程におけるヘパラン硫酸とヘパラナーゼの役割 (2009)
  • Author(s): 平田あずみ、植野高章、松村達志、森谷徳文、菅原利夫、中村浩彰
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-10
• [Presentation] Localization of heparanase during palatal bone formation in palatogenesis in mice (2009)
  • Author(s): Hirata, A. Tsuji, T. Ueno, T. Yamada, T. Imura, H. Kagawa, T. Matsumura, T. Moritani, N. Mishima, K. Sugahara, T. and Nakamura, H.
  • Organizer: 36^<th> European Symposium on Calcified Tissues
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2009-05-24