Mechanical response in artificially controlled osteocyte network

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592594
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Okayama University
• Principal Investigator: KAMIOKA Hiroshi 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (80253219)
• Co-Investigator(Kenkyū-buntansha): SUGAWARA Yasuyo 岡山大学, 大学院・医歯薬学総合研究科, 助教 (70379775) ; KATANOSAKA Yuki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (60432639)
• Project Period (FY): 2009 – 2011
• Keywords: 骨細胞 / ナノパターニング / 機械的刺激

Research Abstract

Osteocyte in bone are thought to be mechanosensory cells. They form cellular network. However, the role of osteocyte network is not well known. We employed the nano-patterning method to form artificially-formed osteocyte network and applied mechanical stress with fluid shear stress in micro-fluid flow chamber.

Research Products

• [Journal Article] In situ imaging of the autonomous intracellular Ca2+ oscillations of osteoblasts and osteocytes in bone (2012)
  • Author(s): Ishihara Y, Sugawara Y, Kamioka H, Kawanabe N, Kurosaka H, Naruse K, Yamashiro T
  • Journal Title: Bone Volume: 50(4) Pages: 842-52
  • Peer Reviewed
• [Journal Article] 骨細胞による機械的刺激の感知 (2012)
  • Author(s): 上岡寛、山城隆
  • Journal Title: Clinical Calcium Volume: 21巻5号 Pages: 97-104
  • Peer Reviewed
• [Journal Article] Roles of heparan sulfate sulfation in dentinogenesis (2012)
  • Author(s): Hayano H, Kurosaka H, Yanagita T, Kalus I, Fabian Milz, Ishihara Y, Islam MN, Kawanabe N, Saito M, Kamioka H, Adachi T, Dierks T, Yamashiro T
  • Journal Title: J Biol Chem Volume: 287(15) Pages: 12217-29
  • Peer Reviewed
• [Journal Article] Stage-specific embryonic antigen-4 identifies human dental pulp stem cells (2012)
  • Author(s): Kawanabe N, Murata S, Fukushima H, Ishihara Y, Yanagita E, Ono M, Kurosaka H, Kamioka H, Itoh T, Kuboki T, Yamashiro T
  • Journal Title: Exp Cell Res Volume: 318(5) Pages: 453-463
  • Peer Reviewed
• [Journal Article] A case report of a patient with nonsyndromic bilateral and multiple impacted teeth and dentigerous cysts (2012)
  • Author(s): Ishihara Y, Kamioka H, Takano-Yamamoto T, Yamashiro T
  • Journal Title: American Journal of Orthodontics and Dentofacial Orthopedics Volume: 141(2) Pages: 228-241
  • Peer Reviewed
• [Journal Article] Intradermal injection of botulinum toxin type A alleviates infraorbital nerve constriction-induced thermal hyperalgesia in an operant assay (2012)
  • Author(s): Kumada A, Matsuka Y, Spigelman I, Maruhama K, Yamamoto Y, Neubert JK, Nolan TA, Watanabe K, Maekawa K, Kamioka H, Yamashiro T, Kuboki T, Oguma K
  • Journal Title: J Oral Rehabilitation Volume: 39(1) Pages: 63-72
  • Peer Reviewed
• [Journal Article] Sox9 expression during fracture repair (2011)
  • Author(s): Shintaku Y, Murakami T, Yanagita T, Kawanabe N, Fukunaga T, Matsuzaki K, Matsuoka S, Yoshida Y, Kamioka H, Takano-Yamamoto T, Takada K, Yamashiro T
  • Journal Title: Cells Tissues Organs Volume: 194(1) Pages: 38-48
  • Peer Reviewed
• [Journal Article] The three-dimensional morphometry and cell-cell communication of osteocytes network in chick and mouse embryonic calvaria (2011)
  • Author(s): Sugawara Y, Ando R, Kamioka H, Ishihara Y, Honjo T, Kawanabe N, Kurosaka H, Takano-Yamamoto T, Yamashiro T
  • Journal Title: Calcified Tissue International Volume: 88(5) Pages: 416-424
  • Peer Reviewed
• [Journal Article] Application of bioimaging to osteocyte biology (2010)
  • Author(s): Kamioka H, Adachi T
  • Journal Title: Clinical Review in Bone and Mineral Metabolism Volume: 8(4) Pages: 170-178
  • Peer Reviewed
• [Presentation] マウス大腿骨中の三次元骨細胞ネットワークは外部環境によって変化する (2011)
  • Author(s): 菅原康代、上岡寛、石原嘉人、藤澤直子、山城隆
  • Organizer: 第70回日本矯正歯科学会大会 & 第4回国際会議
  • Place of Presentation: 名古屋市
  • Year and Date: 20111017-20
• [Presentation] 生体ライブイメージングを用いた骨組織中における自律性細胞内カルシウムオシレーションの検討 (2011)
  • Author(s): 石原嘉人、菅原康代、上岡寛、川邉、山城隆
  • Organizer: 第70回日本矯正歯科学会大会 & 第4回国際会議
  • Place of Presentation: 名古屋市
  • Year and Date: 20111017-20
• [Presentation] 骨細胞のバイオイメージングによる機能解析 (2011)
  • Author(s): 上岡寛
  • Organizer: 京都大学再生医科学研究所第8回バイオメカニクスセミナー
  • Place of Presentation: 京都市
  • Year and Date: 2011-11-16
• [Presentation] 骨細胞のイメージングによる機能解析 (2011)
  • Author(s): 上岡寛
  • Organizer: 国立長寿医療研究センター運動器疾患研究部主催セミナー
  • Place of Presentation: 大府市
  • Year and Date: 2011-10-27
• [Presentation] ヒト歯根膜細胞におけるギャップ結合を介した細胞間コミュニケーション (2010)
  • Author(s): 吉川祐介、石原嘉人、今井裕一、川邉紀章、上岡寛、山城隆
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: 横浜市
  • Year and Date: 20100927-29
• [Presentation] 骨組織内ライブイメージングを用いた機械的刺激に対する細胞内カルシウム応答の検討 (2010)
  • Author(s): 石原嘉人, 菅原康代, 上岡寛, 川邉紀章, 成瀬恵治, 山城隆
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: 横浜市
  • Year and Date: 20100927-29
• [Presentation] 力学刺激に対する単離骨細胞の情報伝達特性に関する考察 (2010)
  • Author(s): 青沼有紀、平圭佑、安達泰治、北條正樹、上岡寛
  • Organizer: 第22回バイオエンジニアリング講演会
  • Place of Presentation: 岡山市
  • Year and Date: 20100109-10
• [Presentation] Direct Imaging of Flow-induced Oscillatory Calcium Rise in Living Bone (2010)
  • Author(s): Ishihara Y, Sugawara Y, Kamioka H, Honjo T, Kurosaka H, Kawanabe N, Yamashiro T
  • Organizer: JADR Hatton award competition
  • Place of Presentation: Tokyo
  • Year and Date: 2010-01-25
• [Patent(Industrial Property Rights)] 細胞培養システム (2011)
  • Inventor(s): 上岡寛, 他
  • Industrial Property Rights Holder: 上岡寛, 他
  • Industrial Property Number: 特許、特願2011-K1110184
  • Filing Date: 2011-10-04