Analysis of tooth movement mechanism in TNF receptors deficient chimera mouse

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592607
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: KITAURA Hideki 東北大学, 病院, 講師 (60295087)
• Co-Investigator(Kenkyū-buntansha): YOSHIMATSU Masako 長崎大学, 医学部・歯学部附属病院, 助教 (20420630) ; MASUYAMA Ritsuko 長崎大学, 大学院・医歯薬学総合研究科, 助教 (60297596) ; FUJIMURA Yuji 長崎大学, 大学院・医歯薬学総合研究科, 助教 (70448504)
• Project Period (FY): 2009 – 2011
• Keywords: 歯科矯正学

Research Abstract

We generated chimeric mice using wild type (WT)or TNF receptor deficient (KO)marrow, immunodepleted of T-cells and stromal cells. The samples were reciprocally transplanted into WT or KO mice following lethal irradiation to eliminate native marrow. We established an orthodontic tooth movement model in mice by using a Ni-Ti closed coil spring. We found that orthodontic tooth movement was occurred in chimeric mice.

Research Products

• [Journal Article] Inhibitory effects of IL-12 on experimental tooth movement and root resorption in mice (2012)
  • Author(s): Yoshimatsu, M., Kitaura, H., Fujimura, Y., Kohara, H., Morita, Y., Eguchi, T., Yoshida, N
  • Journal Title: Arch. Oral Biol Volume: 57, 1 Pages: 36-43
  • Peer Reviewed
• [Journal Article] Anti-c-Fms antibody inhibits lipopolysaccharide-induced osteoclastogenesis in vivo (2012)
  • Author(s): Kimura, K., Kitaura, H., Fujii, T., Hakami Z., Takano-Yamamoto T
  • Journal Title: FEMSImmunol. Med. Microbiol Volume: 64, 2 Pages: 219-227
  • Peer Reviewed
• [Journal Article] Comparative Proteomic Analysis of a Cytosolic Fraction from β 3 Integrin-deficient Cells (2012)
  • Author(s): Bush, JA., Kitaura, H., Ma, Y., Teitelbaum, SL., Ross, FP., Smith JW.
  • Journal Title: Cancer Genomics Proteomics Volume: 9, 1 Pages: 1-13
  • Peer Reviewed
• [Journal Article] IL-12-and L-18-mediated nitric oxide induced apoptosis of adherent bone marrow cells in TNF-a-induced osteoclast formation (2012)
  • Author(s): Kitaura, H., Aonuma, T., Fukumoto, E., Kimura, K., Fujii, T., Hakami, Z., Takano-Yamamoto T
  • Journal Title: Interface Oral Health Science 2011 Pages: 125-127
• [Journal Article] IFN-γ directly inhibits TNF-α-induced osteoclastogenesis in vitro and in vivo and induces apoptosis mediated by Fas/Fas ligand interaction (2011)
  • Author(s): Kohara, H., Kitaura, H., Fujimura, Y., Yoshimatsu, M., Morita, Y., Eguchi, T., Masuyama, R., Yoshida, N.
  • Journal Title: Immunol. Lett Volume: 137, 1-2 Pages: 53-61
  • Peer Reviewed
• [Journal Article] IL-12-and IL-18-mediated nitric oxide-induced apoptosis in TNF-α-mediated osteoclastogenesis of bone marrow cells (2011)
  • Author(s): Kitaura, H., Fujimura, Y., Yoshimatsu, M., Kohara, H., Morita, Y., aonuma, T., Fukumoto, E., Masuyama, R., Yoshida, N., Takano-Yamamoto T
  • Journal Title: Calcif. Tissue Int Volume: 89, 1 Pages: 65-73
  • Peer Reviewed
• [Journal Article] Overexpression of bcl2 in osteoblasts inhibits osteoblast differentiation and induces osteocyte apoptosis (2011)
  • Author(s): Moriishi, T., Maruyama, Z., Fukuyama, R., Ito, M., Miyazaki, T., Kitaura, H., Ohnishi, H., Furuichi, T., Kawai, Y., Masuyama, R., Komori, H., Takada, K., Kawaguchi, H., Komori, T
  • Journal Title: PLoS One Volume: 6, 11 Pages: e27487
  • Peer Reviewed
• [Journal Article] TNF-αの骨代謝作用 (2011)
  • Author(s): 北浦英樹
  • Journal Title: 骨粗鬆症治療 Volume: 10, 1 Pages: 10-14
• [Journal Article] 特集カラーレビュー骨髄細胞でのTNF-aによる破骨細胞形成におけるIL-12 およびIL-18 によるアポトーシス誘導 (2011)
  • Author(s): 北浦英樹
  • Journal Title: 骨粗鬆症治療 Volume: 10, 1 Pages: 8
• [Journal Article] IL-18 Inhibits TNF-a-induced osteoclastogenesis possibly via a T cell-independent mechanism in synergy with IL-12 in vivo (2010)
  • Author(s): Morita, Y., Kitaura, H., Yoshimatsu, M., Fujimura, Y., Kohara, H., Eguchi, T., Yoshida, N
  • Journal Title: Calcif. TissueInt Volume: 86, 3 Pages: 242-248
  • Peer Reviewed
• [Journal Article] A M-CSF receptor c-Fms antibody inhibits mechanical stress-induced root resorption during orthodontic tooth movement in mice (2009)
  • Author(s): Kitaura, H., Fujimura, Y., Yoshimatsu, M., Eguchi, T., Kohara, H., Insan, J., Morita, Y., Yoshida, N
  • Journal Title: Angle Orthod Volume: 79, 5 Pages: 835-841
  • Peer Reviewed
• [Journal Article] Influence of bisphosphonates on orthodontic tooth movement in mice (2009)
  • Author(s): Fujimura, Y., Kitaura, H., Yoshimatsu, M., Eguchi, T., Kohara, H., Morita, Y., Yoshida, N.
  • Journal Title: Eur. J. Orthod Volume: 31, 6 Pages: 572-577
  • Peer Reviewed
• [Journal Article] IL-12 inhibits TNF-α induced osteoclastogenesis via a T cell-independent mechanism in vivo (2009)
  • Author(s): Yoshimatsu, M., Kitaura, H., Fujimura, Y., Eguchi, T., Kohara, H., Morita, Y., Yoshida, N.
  • Journal Title: Bone Volume: 45, 5 Pages: 1010-1016
  • Peer Reviewed
• [Journal Article] Calcium/calmodulin-signaling supports TRPV4 activation in osteoclasts and regulates bone mass
  • Author(s): Masuyama R, Mizuno A, Komori H, Kajiya H, Uekawa A, Kitaura H, Okabe K, Ohyama K, Komori T
  • Journal Title: J. Bone Miner. Res Volume: (in press)
  • Peer Reviewed
• [Presentation] Effect of SHIP on TNF-alpha-induced osteoclastogenesis (2011)
  • Author(s): Yoshimatsu M, Kitaura H, Fujimura Y, Kohara H, Morita Y, Eguchi T, Yoshida N.
  • Organizer: The 4th International Congress and The 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 20111017-20
• [Presentation] Anti-c-Fms antibody inhibits lipopolysaccharide-induced osteoclastogenesis in vivo (2011)
  • Author(s): Kimura, K., Kitaura, H., Fujii, T., Hakami Z., Takano-Yamamoto T
  • Organizer: The 4th International Congress and The 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 20111017-20
• [Presentation] Interleukin-4 inhibits tumor necrosis factor-α-mediated osteoclast formation in vivo (2011)
  • Author(s): Fujii T, Kitaura H, Kimura K, Hakami Z, Takano-Yamamoto T
  • Organizer: The 4th International Congress and The 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 20111017-20
• [Presentation] IL-12-and IL-18-mediated nitric oxide-induced apoptosis in TNF-α-mediated osteoclastogenesis (2011)
  • Author(s): Kitaura H, Fujimura Y, Yoshimatsu M, Kohara H, Morita Y, Aonuma T, Fukumoto E, Yoshida N, Takano-Yamamoto T
  • Organizer: The 4th International Congress and The 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 20111017-20
• [Presentation] IL-12-and IL-18-mediated nitric oxide induced apoptosis of adherent bone marrow cells in TNF-α-induced osteoclast formation (2011)
  • Author(s): Kitaura H, Aonuma T, Fukumoto E, Kimura K, Fujii T, Hakami Z, Takano-Yamamoto T
  • Organizer: The 4th International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: Sendai, Japan
  • Year and Date: 20110307-08
• [Presentation] IL-18 inhibits TNF-a-induced osteoclastogenesis possibly via a T cell-independent mechanism in synergy with IL-12 in vitro and in vivo (2009)
  • Author(s): Morita Y, Kitaura H, Kohara H, Fujimura Y, Yoshimatsu M, Eguchi T, Yoshida N
  • Organizer: The 6th Japan-Korea conference on cellular signaling for young scientists
  • Place of Presentation: Nagasaki, Japan
  • Year and Date: 20091124-26
• [Presentation] IFN-γ. mediated nitric oxide inhibits proliferation of M-CSF. dependent bone marrow macrophages (2009)
  • Author(s): Kohara H, Kitaura H, Fujimura Y, Yoshimatsu M, Morita Y, Eguchi T, Takano-Yamamoto T, Yoshida N
  • Organizer: The 31st Annual Meeting of the American Society for Bone and Mineral Research.Denver
  • Place of Presentation: U. S. A
  • Year and Date: 20090911-15
• [Presentation] IL-18 inhibits TNF-α-induced osteoclastogenesis in synergy with IL-12 in vivo (2009)
  • Author(s): Morita Y, Kitaura H, Fujimura Y, Yoshimatsu M, Kohara H, Eguchi T, Takano-Yamamoto T, Yoshida N
  • Organizer: The 31st Annual Meeting of the American Society for Bone and Mineral Research.Denver
  • Place of Presentation: U. S. A
  • Year and Date: 20090911-15
• [Presentation] Takano-Yamamoto T, Yoshida N. Effect of Bisphosphonates on Orthodontic Tooth Movement in Mice (2009)
  • Author(s): Fujimura Y, Kitaura H, Yoshimatsu M, Kohara H, Morita Y, Eguchi T
  • Organizer: The 31st Annual Meeting of the American Society for Bone and Mineral Research.Denver
  • Place of Presentation: U. S. A
  • Year and Date: 20090911-15
• [Presentation] IL-12 inhibits TNF-α mediated osteoclastogenesis via a T cell-independent mechanism in vivo (2009)
  • Author(s): Yoshimatsu M, Kitaura H, Fujimura Y, Kohara H, Morita Y, Eguchi T, Takano-Yamamoto T, Yoshida N
  • Organizer: The 31st Annual Meeting of the American Society for Bone and Mineral Research.Denver
  • Place of Presentation: U. S. A
  • Year and Date: 20090911-15
• [Presentation] IL-12-and IL-18-mediated Nitric Oxide Induces Apoptosis in TNF-α-induced Osteoclastogenesis of Bone Marrow Cells (2009)
  • Author(s): Kitaura H, Fujimura Y, Yoshimatsu M, Kohara H, Morita Y, Eguchi T, Yoshida N, Takano-Yamamoto T
  • Organizer: The 31st Annual Meeting of the American Society for Bone and Mineral Research.Denver
  • Place of Presentation: U. S. A
  • Year and Date: 20090911-15