Development of the diagnostic method for periodontal diseases Using a microdevice to determine calprotectin

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592625
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: KIDO Junichi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授 (10195315)
• Co-Investigator(Kenkyū-buntansha): NAGATA Toshihiko 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (10127847) ; BANDO Mikado 徳島大学, 病院, 医員 (10510000)
• Project Period (FY): 2009 – 2011
• Keywords: 歯周病診断 / カルプロテクチン / マイクロデバイス

Research Abstract

The purpose of this study is a development of a microdevice system to determine calprotectin in gingival crevicular fluid(GCF) with a small volume. We discharged an anti-calprotectin antibody on a microchip using an inkjet printing system and made the system to determine calprotectin in GCF using a microchip ELISA method. This method enabled to save GCF sample and antibody to one-fifty and a time one-five compared to a conventional ELISA method. This method to determine calprotectin in GCF may be useful for diagnosis of periodontal diseases.

Research Products

• [Journal Article] Determination of calprotectin in gingival crevicular fluid by immunoassay on a microchip (2012)
  • Author(s): J Kido, K Abe, S Yatsushiro, M, Bando, Y Hiroshima, T Nagata, T Ooie, M Tanaka, M Kataoka
  • Journal Title: Clin Biochem
  • DOI: DOI:10.1016/j.clinbiochem.2012.05.009
  • Peer Reviewed
• [Journal Article] Diagnosis of periodontal diseases by biomarkers (2012)
  • Author(s): J Kido, M Hino, M Bando, Y Hiroshima
  • Journal Title: Electrical Engineering in Japan Volume: 179 Pages: 233-237
  • Peer Reviewed
• [Journal Article] Analysis of proteins in human gingival crevicular fluid by mass spectrometry (2011)
  • Author(s): J Kido, M Bando, Y Hiroshima, H Iwasaka, K Yamada, N Ohgami, T Nambu, M Kataoka, T Yamamoto, Y Shinohara, I Sagawa, T Nagata
  • Journal Title: J Periodont Res
  • DOI: DOI:10:1111/j.1600-0765.2011.01458.x
  • Peer Reviewed
• [Journal Article] Resistin in gingival crevicular fluid and induction of resistin release by Porphyromonas gingivalis lipopolysaccharide in human neutrophils (2011)
  • Author(s): Y Hiroshima, M Bando, Y Inagaki, C Mihara, M Kataoka, H Murata, Y Shinohara, T Nagata, J Kido
  • Journal Title: J Periodont Res
  • DOI: DOI:10.1111/j.1600-0765.2011.01466.x
  • Peer Reviewed
• [Presentation] バイオマーカーを用いた糖尿病関連歯周炎の診断研究 (2012)
  • Author(s): 木戸淳一, ほか
  • Organizer: 第28回歯科医学を中心とした総合的な研究を推進する集い
  • Place of Presentation: 日本歯科医師会館(東京都)
  • Year and Date: 2012-01-07
• [Presentation] マイクロチップELISA法による歯肉溝滲出液中のカルプロテクチンの測定 (2011)
  • Author(s): 木戸淳一, ほか
  • Organizer: 第54回秋季日本歯周病学会学術大会
  • Place of Presentation: 海峡メッセ下関(下関市)
  • Year and Date: 2011-09-24
• [Presentation] 歯肉溝滲出液中のYKL-40の分析 (2011)
  • Author(s): 坂東由記子, ほか
  • Organizer: 第54回秋季日本歯周病学会学術大会
  • Place of Presentation: 海峡メッセ下関(下関市)
  • Year and Date: 2011-09-24
• [Presentation] 歯肉溝滲出液中のグリコアルブミン測定法の確立 (2011)
  • Author(s): 板東美香, ほか
  • Organizer: 第54回春季日本歯周病学会学術大会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2011-05-27