Analysis of association between gene polymorphisms of antimicrobial peptides and periodontitis among Japanese patients

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592626
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: INAGAKI Inagaki 徳島大学, 病院, 助教 (50380019)
• Co-Investigator(Kenkyū-buntansha): NAGATA Toshihiko 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (10127847) ; BANDO Mika 徳島大学, 病院, 助教 (10510000)
• Project Period (FY): 2009 – 2011
• Keywords: 歯周病 / 抗菌ペプチド / 遺伝子多型 / カルプロテクチン / ディフェンシン / ラクトフェリン / SNPs / インベーダー法

Research Abstract

To investigate the relationship between single-nucleotide polymorphisms(SNPs) of antimicrobial peptides and periodontitis, we performed to genotype at known 7 SNPs(three SNPs of calprotectin, three SNPs of.-defensin-1 and a SNP of lactoferrin) in 103 Japanease individuals with or without periodontitis. The-44CC genotype of DEFB1(rs1800972) is associated with moderate and severe chronic periodonntits(odds ratio 3. 692). Furthermore.-defensin-1 in gingival crevicular fluids were quantified by ELISA, the peptide concentrations were significantly lower in the individuals with-44 CC genotype of DEFB1 than in those without-44CC genotype of it.