2011 Fiscal Year Final Research Report
Study on novel tissue regenerative therapy using bone marrow-derived mesenchymal stem cells and FGF/Phosphophoryn/Collagen complex
| Project/Area Number |
21592630
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Matsumoto Dental University (2011)
Health Sciences University of Hokkaido (2009-2010) |
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Principal Investigator |
FUJII Takeo 松本歯科大学, 歯学部・附属病院, 臨床教授 (30173389)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Takashi 北海道医療大学, 歯学部, 教授 (40265070)
ABIKO Yoshihiro 北海道医療大学, 歯学部, 教授 (90260819)
SAITO Masato 北海道医療大学, 歯学部, 教授 (50337036)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 歯周外科学 / 再生治療 |
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| Research Abstract |
With the goal to establish new tissue regenerative therapy for extensive regeneration of destroyed periodontal tissues accompanying vertical or horizontal bone defects due to severe periodontitis, The aim of this study was to evaluate the kinetics of bone marrow-derived mesenchymal stem cells cultured in a FGF. collagen complex as a regenerative scaffold in vitro. Bone marrow-derived mesenchymal stem cells showed good growth in the collagen gel culture. The effect of FGF in promoting cell growth is exhibited at the early stage of culture, and the ALP activity has already passed the peak when assayed at 14 days of culture at FGF concentrations of 10 ng/ml. The ALP activity showed in the presence of phosphophoryn in a dose-dependent manner at 14 days of culture. A marked difference in calcium content was not observed. The present study sheds light on the development of an optimal biomaterial that forms the basis of calcified hard tissue formation, suggesting the feasibility of applying the 3-D collagen gel cell culture system for the construction of a scaffold that controls the amount of regenerating tissue.
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Research Products
(5 results)
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[Journal Article] Murakami FGF-2 stimulates periodontal regeneration : results of multicenter randomized clinical trial2011
Author(s)
Kitamura M, Akamatsu M, Machigashira M, Hara Y, Sakagami R, Hirofuji T, Hamachi T, Maeda K, Yokota M, Kido J, Nagata T, Kurihara H, Takashiba S, Sibutani T, Fukuda M, Noguchi T, Yamazaki K, Yoshie H, Ioroi K, Arai T, Nakagawa T, Ito K, Oda S, Izumi Y, Ogata Y, Yamada S, Shimauchi H, Kunimatsu K, K awanami M, Fujii T, Furuichi Y, Furuuchi T, Sasano T, Imai E, Omae M, Yamada S, Watanuki M
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Journal Title
J Dent Res
Volume: 90(1)
Pages: 35-40
Peer Reviewed
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