Study on anti. inflammatory action of endopeptidases for prevention and treatment of periodontal diseases

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592657
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: AWANO Shuji 九州歯科大学, 歯学部, 講師 (20301442)
• Co-Investigator(Kenkyū-buntansha): ANSAI Toshihiro 九州歯科大学, 歯学部, 教授 (80244789) ; SOU Inho 九州歯科大学, 歯学部, 助教 (10285463) ; TAKEHARA Tadamichi 九州歯科大学, 歯学部, 名誉教授 (00038879) ; YOSHIDA Akihiro 九州歯科大学, 歯学部, 助教 (20364151)
• Project Period (FY): 2009 – 2011
• Keywords: エンドペプチダーゼ / NEP / サブスタンスP / インターロイキン-1 / 歯周疾患 / ケラチノサイト

Research Abstract

This project verified the role of neutral(NEP) and endothelin. converting enzyme-1(ECE-1) in oral epithelial keratinocyte(hOMK). As a result, NEP has been found to regulate the levels of inflammatory mediators such substance P and interleukin-1βgenerated from hOMK with ECE-1, and it is suggested that NEP and ECE-1 on hOMK have anti-inflammatory action during the inflammatory process of periodontal diseases.

Research Products

• [Journal Article] Physical fitness and 6.5-year mortality in an 85-year old community-dwelling population (2012)
  • Author(s): Takata, Y., Ansai, T., Soh, I., Awano, S., Yoshitake, Y., Kimura, Y., Nakamichi, I., Goto, K., Fujisawa, R., Sonoki, K., Yoshida, A., Toyoshima, K. and Nishihara, T
  • Journal Title: Arch Gerontol Geriatr Volume: 54(1) Pages: 28-33
• [Journal Article] Association of disease-specific mortality with physical fitness measurements and nonparticipation in an 80-year-old population. (2012)
  • Author(s): Takata Y, Ansai T, Soh I, Awano S, Yoshitake Y, Kimura Y, Nakamichi I, Akifusa S, Goto K, Yoshida A, Fujisawa R, Sonoki K, Nishihara T
  • Journal Title: Geriatrics
• [Journal Article] Salivaryα-amylase activity and stress in Japan Air Self-Defence Force cargo pilots involved in Iraq reconstruction (2012)
  • Author(s): Iizuka N, Awano S, Ansai T
  • Journal Title: American Journal of Human Biology
  • DOI: doi:10.1002/ajhb.22247
• [Journal Article] Oral health in patients on haemodialysis for diabetic nephropathy and chronic glomerulonephritis (2012)
  • Author(s): Teratani G, Awano S, Soh I, Yoshida A, Kinoshita N, Hamasaki T, Takata Y, Sonoki K, Nakamura H, Ansai T
  • Journal Title: Clin Oral Investig
• [Journal Article] Streptococcus anginosus L-cysteine desulfhydrase gene expression is associated with abscess formation in BALB/c mice (2011)
  • Author(s): Takahashi, Y., Yoshida, A., Nagata, E., Hoshino, T., Oho, T., Awano, S., Takehara, T. and Ansai, T
  • Journal Title: Molecular Oral Microbiol Volume: 26 Pages: 221-227
• [Journal Article] Correlations between health status and OralChroma.-determined volatile sulfide levels in mouth air of the elderly (2011)
  • Author(s): Awano S, Takata Y, Soh I, Yoshida A, Hamasaki T, Sonoki K, Ohsumi T, Nishihara T, Ansai T
  • Journal Title: J Breath Res Volume: 5(4) Pages: 046007
• [Journal Article] Enumeration of viable Enterococcus faecalis, a predominant apical periodontitis pathogen, using propidium monoazide and quantitative real-time polymerase chain reaction (2011)
  • Author(s): Takahashi Y, Yoshida A, Nagayoshi M, Kitamura C, Nishihara T, Awano S, Ansai T
  • Journal Title: Microbiol Immunol Volume: 17
  • DOI: doi:10.1111/j.1348-0421.2011.00390.x
• [Journal Article] Rapid LED-based fluorescence microscopy distinguishes between live and dead bacteria in oral clinical samples (2010)
  • Author(s): Masakiyo, Y., Yoshida, A., Takahashi, Y., Shintani, Y., Awano, S., Ansai, T., Sawayama, S., Shimakita, T. and Takehara, T
  • Journal Title: Biomed Res Volume: 31 Pages: 21-26
• [Journal Article] Relationship between endothelin-1 and interleukin-1 beta in inflamed periodontal tissues (2009)
  • Author(s): Rikimaru, T., Awano, S., Mineoka, T., Yoshida, A., Ansai, T. and Takehara, T
  • Journal Title: Biomed Res Volume: 30 Pages: 349-355
• [Journal Article] Determination of cortisol and dehydroepiandrosterone levels in saliva for screening of periodontitis in older Japanese adults
  • Author(s): Ansai, T., Soh, I., Ishisaka, A., Yoshida, A., Awano, S., Hamasaki, T., Sonoki, K., Takata, Y. and Takehara, T
  • Journal Title: Int J Dent Volume: 280737 Pages: 8
• [Presentation] 粘膜上皮におけるメタロプロテアーゼの遺伝子発現レベルと歯周健康状態との関係 (2011)
  • Author(s): 木下直昌、粟野秀慈、中田元希、吉田明弘、邵仁浩、峰岡哲郎、安細敏弘
  • Organizer: 第60回日本口腔衛生学会総会
  • Place of Presentation: 松戸
  • Year and Date: 20111008-10
• [Presentation] 培養口腔ケラチノサイトにおけるneutral endopeptidaseの作用について (2011)
  • Author(s): 中田元希、粟野秀慈、木下直昌、峰岡哲郎、吉田明弘、邵仁浩、安細敏弘
  • Organizer: 第60回日本口腔衛生学会総会
  • Place of Presentation: 松戸
  • Year and Date: 20111008-10
• [Presentation] 培養口腔ケラチノサイトにおけるneutral endopeptidaseとsubstance Pとの関係について (2011)
  • Author(s): 中田元希, 粟野秀慈, 木下直昌, 峰岡哲郎、吉田明弘、邵仁浩、安細敏弘
  • Organizer: 第71回九州歯科学会総会
  • Place of Presentation: 北九州
  • Year and Date: 20110528-29
• [Presentation] 頬粘膜上皮で発現するメタロプロテアーゼ遺伝子に関するパイロット研究 (2011)
  • Author(s): 木下直昌、粟野秀慈、中田元希、吉田明弘、邵仁浩、峰岡哲郎、安細敏弘
  • Organizer: 第71回九州歯科学会総会
  • Place of Presentation: 北九州
  • Year and Date: 20110528-29
• [Presentation] Neutral Endopeptidase and Endothelin-converting Enzyme in Human Buccal Mucosal Epithelium (2010)
  • Author(s): Kinoshita, N., Awano, S., Yoshida, A., Soh, I., Mineoka, T., and Ansai, T
  • Organizer: 58th Japanese Association for Dental Research
  • Place of Presentation: Kitakyushu
  • Year and Date: 20101120-21
• [Remarks]
  • URL: http://www2.kyu-dent.ac.jp/dept/oral-health/