2011 Fiscal Year Final Research Report
Self-resistance mechanism for secondary metabolite-accumulating species-Structure and function of okadaic acid-binding proteins
| Project/Area Number |
21603003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Tohoku University |
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Principal Investigator |
KONOKI Keiichi 東北大学, 大学院・農学研究科, 准教授 (40292825)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAGAKI Tohru サントリー, 生物有機科学研究所 (40313209)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 下痢生貝毒 / オカダ酸 / オカダ酸結合タンパク質 / 自己耐性機構 / クロイソカイメン |
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| Research Abstract |
Okadaic acid binding proteins OABP_s 2. 1 and 2. 3, originally isolated from the sponge Halichondria okadai, were stably expressed as recombinant proteins in E. coli. Dissociation constants of OA from recombinant OABP_s 2. 1 and 2. 3 were determined in the binding assays. The okadaic acid binding proteins were deduced to be the sponge proteins but not those from symbiotic species. Furthermore, we quantitated okadaic acid and OABP2. 1 in the sponge collected from various places in Japan, and speculated a possible self-defense system of the sponge.
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