2011 Fiscal Year Final Research Report
Development of cell chip for examination of radiobiological damage
| Project/Area Number |
21651021
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Osaka University |
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Principal Investigator |
IIDA Toshiyuki 大阪大学, 大学院・工学研究科, 教授 (60115988)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Yushi 大阪大学, 大学院・工学研究科, 准教授 (40224547)
SATO Fuminobu 大阪大学, 大学院・工学研究科, 助教 (40332746)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 応答 / 細胞損傷 / DNA損傷 |
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| Research Abstract |
A cell chip was developed for the examination of biological damage of cells irradiated by high-energyα-rays. A CR-39 track detector was employed as a chip substrate to identify high-energy charged particles traversing target cells. HeLa cancer cells on the chip were irradiated with alpha particles and stained with a fluorescent probe molecule for DNA damage detection. The CR-39 substrate was etched by means of an alkali solution during cell incubation. The HeLa cells andαtracks were successfully observed by microscopy at once
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