2011 Fiscal Year Final Research Report
Development of in situ bioremediation technology to prevent repetition of Minamata disease in the world
| Project/Area Number |
21651038
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Tohoku Gakuin University |
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Principal Investigator |
ENDO Ginrou 東北学院大学, 工学部, 教授 (80194033)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAUCHI Keisuke 東北学院大学, 工学部, 准教授 (20324014)
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| Co-Investigator(Renkei-kenkyūsha) |
KAN Baihou 東北学院大学, 工学総合研究所, 客員研究員 (20533186)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 環境修復技術 |
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| Research Abstract |
In this study, we analyzed merA genes from the soil sample of a mercury contaminated site. The result showed that there is big diversity between merA genes. Second study was conducted to wide range of merA gene analysis. The results showed that there are many new merA genes in the mercury contaminated soil sample. In the final stage of this research, direct interspecies gene transfer was investigated by using filter-mating method and sandwich-meting method. The mercury resistant and tetracycline resistant bacterial colonies were observed on the culture plates. However, those resistant bacteria were not stable in the liquid culture. From these results, it was concluded that interspecies gene transfer of mercury resistance gene needs special vectors for the horizontal gene transfer.
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