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2011 Fiscal Year Final Research Report

Toward the development of biomimetic under-water adhesives inspired by barnacle larval cement

Research Project

  • PDF
Project/Area Number 21651056
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Nanomaterials/Nanobioscience
Research InstitutionAkita Prefectural University

Principal Investigator

OKANO Keiju  秋田県立大学, 生物資源科学部, 教授 (40147070)

Co-Investigator(Kenkyū-buntansha) OZAKI Noriaki  秋田県立大学, 生物資源科学部, 助教 (50468120)
Project Period (FY) 2009 – 2011
Keywordsフジツボ / 幼生 / 水中接着剤 / タンパク質 / ナノ材料
Research Abstract

Toward the development of biomimetic under-water adhesives inspired by larval cement of the barnacle, Megabalanus rosa, we have obtained the following results.
1) We have developed a method to measure macroscopic forces for marine adhesives using an atomic force microscope. 2) We have developed a microassay to measure the interaction between proteins and clay nanosheets in mock-marine environments using Roche glass capillaries. 3) We have prepared two recombinant cement proteins ; rec-Ccg-57k and rec-Ccg-ASP, both of which, however, did not show positive effects on 1) and 2). 4) We found the strong antimicrobial activity of rec-Ccg-57k against marine GRAM-negative bacteria, Planococcus citreus. 5) In the course of searching unidentified cement components using next generation sequencing, we have found a gene homologous to M. rosa adult cement protein gene mrcp-100k.

  • Research Products

    (6 results)

All 2012 2011 2010 2009 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Metamorphosis in balanomorphan, pedunculated and parasitic barnacles : A video based analysis2012

    • Author(s)
      J. Hoeg, D. Maruzzo, K. Okano, H. Glenner and B. Chan
    • Journal Title

      Integrative and Comparative Biology

      Volume: (in Press) Pages: 1-11

    • DOI

      doi:10.1093/icb/ics053

    • Peer Reviewed
  • [Journal Article] Microtexture of larval shell of oyster, Crassostrea nippona : A FIB-TEM study2009

    • Author(s)
      M. Kudo, K. Saruwatari, N. Ozaki, K. Okano, H. Nagasawa and T. Kogure
    • Journal Title

      Journal of Structural Biology

      Volume: 169 Pages: 1-5

    • Peer Reviewed
  • [Presentation] フジツボキプリス幼生が分泌する海洋環境で働く接着剤2011

    • Author(s)
      岡野桂樹
    • Organizer
      第6回LSWシンポジウムソフト & ウェットマターのデザイン・新素材から生物まで
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-01-07
  • [Presentation] フジツボ幼生付着の物質的基盤2010

    • Author(s)
      岡野桂樹
    • Organizer
      2010年度日本農芸化学会大会日本水産学会-農芸化学会合同シンポジウム、水産動物における生理現象とその物質的基盤
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-30
  • [Presentation] フジツボキプリス幼生の付着:行動から分子まで2010

    • Author(s)
      岡野桂樹
    • Organizer
      シンポジウム「動物発生とケミカルバイオロジー」
    • Place of Presentation
      慶応義塾大学
    • Year and Date
      2010-03-14
  • [Remarks]

    • URL

      http://www.akita-pu.ac.jp/bioresource/dbt/cellbiol/OKANOKEIJU/

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Published: 2013-07-31  

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