2011 Fiscal Year Final Research Report
High pressure protein crystal structure analysis
Project/Area Number |
21657027
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Structural biochemistry
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Research Institution | Nagoya University |
Principal Investigator |
WATANABE Nobuhisa 名古屋大学, シンクロトロン光研究センター, 教授 (70212321)
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Project Period (FY) |
2009 – 2011
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Keywords | 生物物理 / X線 / 結晶構造 / 圧力 / ダイヤモンドアンビルセル / 好圧菌 |
Research Abstract |
In order to elucidate the mechanism of pressure adaptation of proteins, we have developed structural studies of proteins by a high-pressure protein crystallography method using a diamond-anvil cell. IPMDH from the deep-sea bacterium Shewanella benthica DB21MT-2 is more tolerant towards HP stress than its counterpart from the land bacterium S. oneidensis MR-1, even though these two enzymes share 85% amino-acid identity. Crystal structures of IPMDH have been determined at about 2 A resolution under pressures ranging from 0. 1 to 650 MPa. Penetration of water molecules into IPMDH is observed at high-pressure, and relationship of the structure and pressure resistance has been elucidated.
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[Journal Article] High-pressure-induced water penetration into 3-isopropylmalate dehydrogenase2012
Author(s)
Nagae, T., Kawamura, T., Chavas, LMG., Niwa, K., Hasegawa, M., Kato, C. and Watanabe, N.
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Journal Title
Acta Cryst
Volume: D68(3)
Pages: 300-309
DOI
Peer Reviewed
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[Presentation] 高圧蛋白質結晶構造解析2011
Author(s)
永江峰幸,河村高志, Leonard Chavas,丹羽健,長谷川正,加藤千明,渡邉信久
Organizer
第28回PFシンポジウム
Place of Presentation
つくば国際会議場
Year and Date
20110712-13
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