Effect of rhizobia primed with flavonoid on growth, root development and nodulation in leguminous cover crops grown under low temperature condition

2011 Fiscal Year Final Research Report

• Project/Area Number: 21658007
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Crop science/Weed science
• Research Institution: Osaka Prefecture University
• Principal Investigator: DAIMON Hiroyuki 大阪府立大学, 生命環境科学研究科, 教授 (50236783)
• Project Period (FY): 2009 – 2011
• Keywords: 環境調和型農林水産 / 植物 / 発現制御 / 作物栽培 / 微生物 / 窒素固定 / 根粒菌 / 作付体系

Research Abstract

In field pea(Pisum sativum) grown in temperate region of western Japan, low temperature under late seeding condition should be considered as a limiting factor for early growth. Of inhibition mechanisms, decreasing flavonoid compounds released from legume roots for nod gene activation might be of importance. Root nodule formation was investigated after inoculation in both flavonoid priming and the control. Root nodule numbers per plant were higher in flavonoid priming than the control. Plants inoculated with primed rhizobia showed alterative root structure such as reduction of the number of the second order lateral roots and the continuous nodule formation along the root axis. These results suggest that priming treatment of rhizobia with flavonoid might induce earlier formation of root nodules due to inhibiting lateral root differentiation, indicating that root system development of pea plants occurs with a trade-off between the nodulation and lateral branching under unsuitable environmental conditions.

Research Products

• [Journal Article] Seasonal changes in nitrogen fixation in legumes and effect of root-deposited nitrogen on nitrogen uptake of the succeeding crop (2011)
  • Author(s): Choi, B. and Daimon, H.
  • Journal Title: Proc. 3rd Sci. Conf. ISOFAR. Pages: 194-197
• [Journal Article] Control of soybean nodule formation by the newly proposed cultivation method of crack fertilization technique (2011)
  • Author(s): IIjima, M., Honjo, H., Izumi, Y., Daimon, H., Tani, T., Hayashi, M., Suzuki, T.
  • Journal Title: Plant Production Science Volume: 14(2) Pages: 202-212
  • Peer Reviewed
• [Journal Article] 果樹園芸生産における緑肥利用と草生管理 (2010)
  • Author(s): 松村篤・大門弘幸
  • Journal Title: 農業および園芸 Volume: 85 Pages: 161-168
• [Journal Article] 緑肥特集を組むにあたって (2010)
  • Author(s): 大門弘幸
  • Journal Title: 農業および園芸 Volume: 85 Pages: 135
• [Journal Article] 湛水処理が水田土壌で栽培した大納言アズキの生育初期における不定根形成に及ぼす影響 (2010)
  • Author(s): 小森二葉・大橋善之・大門弘幸
  • Journal Title: 根の研究 Volume: 19(2) Pages: 43-48
  • Peer Reviewed
• [Presentation] Effect of rhizobia primed with naringenin on root development and nodulation in Pisum sativum L (2011)
  • Author(s): Nakamura, S., Tanaka, Y., Nakanishi, H, Sakamoto, K., Takada, S, Taira, T., Matsumura, A., Daimon, H.
  • Organizer: The 20th Anniversary Symposium of JSRR
  • Place of Presentation: Tokyo
  • Year and Date: 2011-11-06
• [Presentation] 農耕地の持続的生産と作物の根 (2011)
  • Author(s): 大門弘幸
  • Organizer: 根研究会創立20周年記念式典
  • Place of Presentation: 東京
  • Year and Date: 2011-11-05
• [Presentation] ナリンゲニンによる根粒菌プライミングがエンドウの根系発育および根粒形成に及ぼす影響 (2011)
  • Author(s): 中村智・田中康弘・中西久夫・坂本国之・高田智史・平知明・松村篤・大門弘幸
  • Organizer: 第34回根研究集会
  • Place of Presentation: 佐賀
  • Year and Date: 2011-05-14
• [Presentation] 転換畑アズキにおける根系発育と早期播種による湿害回避 (2010)
  • Author(s): 小森二葉・大橋善之・松村篤・大門弘幸
  • Organizer: 第33回根研究集会
  • Place of Presentation: 姫路
  • Year and Date: 2010-11-13
• [Presentation] 湛水処理が水田土壌で栽培したアズキの不定根形成に及ぼす影響 (2009)
  • Author(s): 小森二葉・大橋善之・大門弘幸
  • Organizer: 第228回日本作物学会講演会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-30
• [Book] 作物学用語事典:窒素固定・窒素代謝 (2010)
  • Author(s): 大門弘幸
  • Total Pages: 176, 178
  • Publisher: 農山漁村文化協会
• [Book] 夏作緑肥作物;作物栽培体系「第8巻飼料作物・緑肥作物」
  • Author(s): 松村篤・大門弘幸
  • Publisher: 朝倉書店
• [Remarks]
  • URL: http://www.plant.osakafu-u.ac.jp/～daimon/HOME.html