2011 Fiscal Year Final Research Report
Construction of cDNA library for the elucidation of mechanism occurred in non-toxic mutant of dinoflagellate
| Project/Area Number |
21658075
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Fisheries chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
CHO Yuko 東北大学, 大学院・農学研究科, 助教 (60323086)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 水産学 / プランクトン / 麻痺性貝毒 / 無毒変異 / cDNAライブラリー |
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| Research Abstract |
Single-cell analysis of paralytic shellfish toxins by HPLC with post column fluorescent derivatization revealed the large variety of toxin productivity among cells from the clonal strain of A. tamarense, a PST-producing dinoflagellate. The different growth rate and toxin contents were observed among the cells in the identical culture well at the early stage of incubation after re-isolation. The fundamental data for the construction of the single-cell library was obtained
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