Detection of VSEL cells in umbilical cord blood and development of co-culture system with umbilical cord-derived mesehchymal stem cells

2011 Fiscal Year Final Research Report

• Project/Area Number: 21659069
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: NAGAMURA Tokiko (INOUE Tokiko) 東京大学, 医科学研究所, 講師 (70240736)
• Co-Investigator(Renkei-kenkyūsha): ISHIGE Ikuo 東京大学, 医科学研究所, 特任研究員 (30396957) ; KOBAYASHI Seiichiro 東京大学, 医科学研究所, 助教 (70376622) ; NAGAMURA Fumitaka 東京大学, 医科学研究所, 教授 (90282491) ; OOI Jun 東京大学, 医科学研究所, 助教 (20302606)
• Project Period (FY): 2009 – 2011
• Keywords: 再生医療 / 臍帯 / 組織幹細胞 / ES細胞 / CD34陽性細胞 / 間葉系幹細胞 / VSEL / 臍帯血

Research Abstract

This study is aimed to find the source cells for regenerative medicine, such as Very Small ES like(VSEL) cells in umbilical cord blood(UCB). We found the SSEA3^+very small N/C high-ratio cells, but too low incidence to do further analysis. On the other hand, in umbilical cord(UC)-derived mesenchymal stem cells(MSCs), we found the SSEA3/4+cells with ES-specific gene markers. Not only UCB, but also UC-MSCs may be good candidate source for the regenerative medicine.

Research Products

• [Journal Article] Time from cord blood collection to processing and temperature influence the quality of mononuclear cell products isolated using a density-gradient protocol (2011)
  • Author(s): Miki Yuzawa, Nagamura-Inoue T, Ikuo Ishige, Kazuo Ogami, Tomoki Tamura, Atsuko Takahashi, Hideki Kodo, Satoru Yamaguchi, and Arinobu Tojo
  • Journal Title: The Japan Society of Transfusion Medicine and Cell Therapy.(日本輸血・細胞治療学会誌) Volume: 57 Pages: 139-145
  • URL: http://www.jstmct.or.jp/jstmct/Journal/BackNumber.aspx
• [Journal Article] Japan Cord Blood Bank Network. Cord Blood Transplantation from Unrelated Donors for Children with Acute Lymphoblastic Leukemia in Japan : The Impact of Methotrexate on Clinical Outcomes. (2011)
  • Author(s): Kato K, Yoshimi A, Ito E, Oki K, Hara J, Nagatoshi Y, Kikuchi A, Kobayashi R, Nagamura-Inoue T, Kai S, Azuma H, Takanashi M, Isoyama K, Kato S
  • Journal Title: Biol Blood Marrow Transplant. Volume: 17 Pages: 1814-21
  • DOI: doi:10.1016/j.bbmt.2011.05.013
• [Journal Article] Japanese Cord Blood Bank Network. Outcome of unrelated umbilical cord blood transplantation in 88 patients with primary immunodeficiency in Japan (2011)
  • Author(s): Morio T, Atsuta Y, Tomizawa D, Nagamura-Inoue T, Kato K, Ariga T, Kawa K, Koike K, Tauchi H, Kajiwara M, Hara T, Kato S
  • Journal Title: Br J Haematol Volume: 154 Pages: 363-72
  • DOI: doi:10.1111/j
• [Journal Article] Disease-specific analyses of unrelated cord blood transplant compared with unrelated bone marrow transplant in adult patients with acute leukemia (2009)
  • Author(s): Atsuta Y., Suzuki R., Nagamura-Inoue T., Taniguchi S., Takahashi S., Kai S., Sakamaki H., Kouzai Y., Kasai M., Fukuda T., Azuma H., Takanashi M., Okamoto S., Tsuchida M., Kawa K., Morishima Y., Kodera Y., and Kato S.
  • Journal Title: Blood Volume: 113 Pages: 1631-8
  • DOI: DOI:10.1182/blood-2008-03-147041
• [Journal Article] Comparison of mesenchymal stem cells derived from arterial, venous, and Wharton's jelly explants of human umbilical cord, Int (2009)
  • Author(s): Ishige I., Nagamura-Inoue T., Honda J. M, Harnprasopwat R., Kido M., Sugimoto M., Nakauchi H, Tojo A
  • Journal Title: J of Hematology Volume: 90 Pages: 261-9
  • DOI: DOI:10.1007/s12185-009-0377-3
• [Presentation] 臍帯血からの制御性T細胞の誘導増幅による免疫抑療法の開発 (2011)
  • Author(s): 長村(井上)登紀子,小林誠一郎,尾上和夫,東條有伸
  • Organizer: 第73回日本血液学会総会
  • Place of Presentation: OS-3-36
  • Year and Date: 2011-10-16
• [Presentation] 未分化マーカーSSEA4を発現する臍帯由来MSCsの解析Characterization of human umbilical cord-derived primitive mesenchymal stem cells expressing SSEA4 (2011)
  • Author(s): 何海萍、長村(井上)登紀子、田村友樹、湯沢美紀、東條有伸
  • Organizer: 第73回日本血液学会総会
  • Place of Presentation: 京都
  • Year and Date: 2011-10-14