2011 Fiscal Year Final Research Report
Generation and application of gene knock-out rabbits for the study of atherosclerosis
| Project/Area Number |
21659078
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | University of Yamanashi |
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Principal Investigator |
FAN Jianglin 山梨大学, 大学院・医学工学総合研究部, 教授 (60272192)
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| Co-Investigator(Kenkyū-buntansha) |
KOIKE Tomonari 山梨大学, 大学院・医学工学総合研究部, 助教 (40432158)
SHIBATA Nobumitsu 山梨大学, 大学院・医学工学総合研究部, 医学研究員 (40452130)
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| Co-Investigator(Renkei-kenkyūsha) |
TEZUKA Hideo 山梨大学, 総合分析実験センター, 准教授 (70155456)
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| Project Period (FY) |
2009 – 2011
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| Keywords | CETP / 核移植 / クローン / HDL / 動脈硬化 |
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| Research Abstract |
It is essential to use genetically modified animals for the elucidation of disease-related gene functions and therapeutic candidate proteins in vivo. In this field, transgenic mice are widely used in the medical research. However, due to the differences between mouse and human in lipid metabolism and cardiovascular system, it is considered that mice are not suitable for the research of lipid metabolism and cardiovascular disease of humans. In the current study, we attempted to establish a somatic cell line with cholesteryl ester transfer protein deficiency and then, using nuclear transfer technology, to make cholesteryl ester transfer protein knock-out rabbits.
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[Journal Article] Endothelial lipase mediates hdl levels in normal and hyperlipidemic rabbits2012
Author(s)
Zhang J, Yu Y, Nakamura K, Koike T, Waqar AB, Zhang X, Liu E, Nishijima K, Kitajima S, Shiomi M, Qi Z, Yu J, Graham MJ, Crooke RM, Ishida T, Hirata K, Hurt-Camejo E, Chen YE, Fan J
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Journal Title
J Atheroscler Thromb
Volume: 19
Pages: 213-226
Peer Reviewed
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[Journal Article] Effects of human c-reactive protein on pathogenesis of features of the metabolic syndrome2011
Author(s)
Pravenec M, Kajiya T, Zidek V, Landa V, Mlejnek P, Simakova M, Silhavy J, Malinska H, Oliyarnyk O, Kazdova L, Fan J, Wang J, Kurtz TW
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Journal Title
Hypertension
Volume: 57
Pages: 731-737
Peer Reviewed
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[Journal Article] Human c-reactive protein enhances thrombus formation after neointimal balloon injury in transgenic rabbits2011
Author(s)
Matsuda S, Yamashita A, Sato Y, Kitajima S, Koike T, Sugita C, Moriguchi-Goto S, Hatakeyama K, Takahashi M, Koshimoto C, Matsuura Y, Iwakiri T, Chen YE, Fan J, Asada Y
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Journal Title
J Thromb Haemost
Volume: 9
Pages: 201-208
Peer Reviewed
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[Journal Article] Endotoxin accelerates atherosclerosis independent of complement activation2009
Author(s)
Reifenberg K, Lehr HA, Fan J, Koike T, Wiese E, Kupper I, Sagban TA, Schaefer SC, Zahringer U, Torzewski M, Lackner KJ, Bhakdi S
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Journal Title
Thromb Res
Volume: 123
Pages: 653-658
Peer Reviewed
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[Journal Article] Human c-reactive protein does not promote atherosclerosis in transgenic rabbits2009
Author(s)
Koike T, Kitajima S, Yu Y, Nishijima K, Zhang J, Ozaki Y, Morimoto M, Watanabe T, Bhakdi S, Asada Y, Chen YE, Fan J
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Journal Title
Circulation
Volume: 120
Pages: 2088-2094
Peer Reviewed
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[Journal Article] Expression of human apoaii in transgenic rabbits leads to dyslipidemia : A new model for combined hyperlipidemia2009
Author(s)
Koike T, Kitajima S, Yu Y, Li Y, Nishijima K, Liu E, Sun H, Waqar AB, Shibata N, Inoue T, Wang Y, Zhang B, Kobayashi J, Morimoto M, Saku K, Watanabe T, Fan J
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Journal Title
Arterioscler Thromb Vasc Biol
Volume: 29
Pages: 2047-2053
Peer Reviewed
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