2011 Fiscal Year Final Research Report
Approach to allele specific and regulated gene silencing using the artificial miRNA expression system
Project/Area Number |
21659084
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Human genetics
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Research Institution | Kyushu University |
Principal Investigator |
|
Project Period (FY) |
2009 – 2011
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Keywords | マイクQRNA / マイクQR / 遺伝子変異 / アレル特異的 |
Research Abstract |
The allele specific and regulated gene silencing is needed in various aspects of biological and medical fields. To establish this system, artificial miRNA driven by the poly II promoter was designed for discrimination between normal and mutated alleles in silencing effect. Although the allele specific silencing was obtained using artificial miRNA, there was no increased silencing effect expected to be provided by co-expression of another miRNA targeting the region shared by two genotypes. Cre dependent silencing of Smad2 and Smad3 as a model was obtained in ES cells using the construct in which expression of two miRNA species is blocked by the Neo^r gene flanked by two loxP sites.
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[Journal Article] Partial SPAST and DPY30 deletions in a Japanese spastic paraplegia type 4 family2011
Author(s)
Miura, S., Shibata, H., Kida, H., Noda, K., Toyama, T., Iwasaki, N., Iwaki, A., Ayabe, M., Aizawa, H., Taniwaki, T. and Fukumaki, Y.
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Journal Title
Neurogenetics
Volume: 12
Pages: 25-31
DOI
Peer Reviewed
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[Journal Article] Multiple system degeneration with basophilic inclusions in Japanese ALS patients with FUS mutation2010
Author(s)
Tateishi, T., Hokonohara, T., Yamasaki, R., Miura, S., Kikuchi, H., Iwaki, A., Tashiro, H., Furuya, H., Nagara, Y., Ohyagi, Y., Nukina, N., Iwaki, T., Fukumaki, Y. and Kira, JI.
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Journal Title
Ac to Neuropathol
Volume: 119
Pages: 355-364
DOI
Peer Reviewed
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[Presentation] Linkageassisted exome sequencing to identify the responsible variant for a novel type of hereditary motor and sensory neuropathy with proximal dominancy in the lower extremities found in a single Japanese family2012
Author(s)
Shibata, H., Miura, S., Kida, H., Neda, K., Kaku, Y., Iwaki, A., Ayabe, M., Taniwaki, T. and Fukumaki, Y.
Organizer
Human Genome Meeting 2012
Place of Presentation
Sydney, Australia
Year and Date
20120311-14
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[Presentation] Exome sequencing approach to identify the responsible variant for a novel type of hereditary motor and sensory neuropathy with proximal dominancy in the lower extremities found in a Japanese descent2011
Author(s)
Shibata, H., Miura, S., Kida, H., Neda, K., Kaku, Y., Iwaki, A., Ayabe, M., Taniwaki, T. and Fukumaki, Y.
Organizer
The 12th International Congress of Human Genetics/The 61st Annual Meeting of The American Society of Human Genetics
Place of Presentation
Montreal, Canada
Year and Date
20111011-15
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[Presentation] A novel type of hereditary motor and sensory neuropathy with proximal dominancy in the lower extremities found in a Japanese descent2010
Author(s)
Miura, S., Shibata, H., Neda, K., Y. Kaku, Y. Kida, H., Iwaki, A., Ayabe, M., Aizawa, H., Taniwaki, T. and Fukumaki, Y.
Organizer
135th Annual Meeting of American Neurological Association
Place of Presentation
San Francisco, CA, USA
Year and Date
20101102-06
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[Presentation] Partial SPAST and DPYso deletions in autosomal dominant hereditary spastic paraplegia2010
Author(s)
Miura, S., Shibata, H., Kida, K. Neda, A. Iwaki, A., M. Ayabe, M., H. Aizawa, H., T. Taniwaki, T. and Fukumaki, Y. X
Organizer
60th Annual Meeting of The American Society of Human Genetics
Place of Presentation
Washington, DC, USA
Year and Date
20101102-06
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[Presentation] Haplotype analysis of fukutin(FICTN) gene in two very mild Fukuyama type congenital muscular dystrophy(FCMD)2010
Author(s)
Furuya, H., Arahata, H., Fuji i, N., Miura, S., Shibata, H. and Fukumaki, Y.
Organizer
135th Annual Meeting of American Neurological Association
Place of Presentation
San Francisco, CA, USA
Year and Date
20100912-15
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