2011 Fiscal Year Final Research Report
Analysis of regulatory mechanisms of type 1 diabetes by B cells, based on the AIRE research
| Project/Area Number |
21659230
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Metabolomics
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KURISAKI Hironori 九州大学, 医学研究院, 助教 (70403962)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 自己免疫調節(AIRE)遺伝子 / B細胞 / 自己免疫膵炎 / 1型糖尿病 |
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| Research Abstract |
Since we have already reported that B cells are essential to the progression of insulitis and diabetes in NOD mice(Int Immunol 1997), the exact mechanisms and the role of B cells and aire gene have been studied in Balb/c mice as well as NOD mice. It was found that aire gene was highly expressed in B cells irrespective of the mouse strains. In addition, we found the 64kd novel autoantibody in aire gene knockout Balb/c mice with autoimmune pancreatitis.
On the other hand, in human B cells, accumulating data have shown that expressed AIRE proteins in B cells had two different sizes, indicating the expressed AIRE proteins in B cells might have multiple functions, possibely due to different splicing patterns. We further intend to analyze the role of AIRE protein s expressed in B cells using immunoprocipitation assay and also chromatin-immunoprrecipitation method to identify the interacting proteins and/or genetic regions which may play transcriptional regulation of autoimmune-associated target genes.
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