2011 Fiscal Year Final Research Report
Development of BiTE antibody against pancreatic cancer stem cell
| Project/Area Number |
21659324
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | 膵臓外科学 |
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| Research Abstract |
Bi-specific T-cell engagers(BiTEs) are artificial bispecific monoclonal antibodies that are investigated for the use as anti-cancer reagents. They direct a host's immune system, more specifically the T cells' cytotoxic activity, against cancer cells. BiTEs form a link between T cells and tumor cells. This causes T cells to exert cytotoxic activity on tumor cells by producing proteins like perforin and granzymes, independently of the presence of MHC I or co-stimulatory molecules. These proteins enter tumor cells and initiate the cell's apoptosis. This action mimics physiological processes observed during T cell attacks against tumor cells. In this study, we tried to generate BiTEs that could recognize pancreatic cancer stem cells. To this end, we extensively investigate several markers for pancreatic cancer stem cells including PSCA, HVEM, CD133, CD24, CD44, CD90, CD166, ESA, SHH, BMI-1 using immunohistochemical analysis. However, the expressions of those molecular markers were relatively low in human pancreatic cancer tissues. In addition, the reproducibilities of those results were not confirmed. Therefore, further investigations will be required.
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