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2011 Fiscal Year Final Research Report

Therapeutic benefits of bone marrow stem cells after global cerebral ischemia

Research Project

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Project/Area Number 21659344
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionSapporo Medical University

Principal Investigator

MIYATA Kei  札幌医科大学, 医学部, 助教 (80438051)

Co-Investigator(Kenkyū-buntansha) HOUKIN Kiyohiro  北海道大学, 大学院・医学研究科, 教授 (90229146)
Project Period (FY) 2009 – 2011
Keywords移植・再生医療 / 再生医学 / 神経科学 / 脳・神経 / 発生・分化
Research Abstract

Although intravenous delivery of mesenchymal stem cells(MSC_s) prepared from bone marrow reduces infarction size and ameliorates functional deficits in rat middle cerebral artery occlusion models, there are few reports of MSC treatment in global cerebral ischemia. We utilized a global cerebral ischemia model induced by arresting the heart with a combination of hypovolemia and intracardiac injections of a cold potassium chloride solution in order to study the potential therapeutic benefits of MSC_s on global cerebral ischemia. MSCs were intravenously injected into the rats after resuscitation from cardiac arrest. The effects on structural and functional outcome of MSC were assessed using magnetic resonance spectroscopy(MRS), histology, and cognitive functional analysis. Intravenous delivery of MSC_s reduced the Lac/Cr ratios, nuclear DNA fragmentation, neuronal loss, and elicited functional improvement compared with the control sham group. Enzyme-linked immunosorbent assay(ELISA) of the hippocampus revealed an increase in BDNF in MSC-treated group. These data suggest that intravenous delivery of MSC may have a therapeutic effect in global cerebral ischemia.

  • Research Products

    (2 results)

All 2010 2009

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] Therapeutic benefits of human mesenchymal stem cells derived from bone marrow after global cerebral ischemia2010

    • Author(s)
      Zheng W, Honmou O, Miyata K, Harada K, Suzuki J, Liu H, Houkin K, Hamada H, Kocsis JD
    • Journal Title

      Brain Res

      Volume: 1310 Pages: 8-16

    • Peer Reviewed
  • [Presentation] 心配停止後全脳虚血に対する骨髄幹細胞移植の検討2009

    • Author(s)
      鄭偉、本望修、原田邦明、鈴木順平、宝金清博
    • Organizer
      第18回日本意識障害学会
    • Place of Presentation
      川越
    • Year and Date
      2009-07-24

URL: 

Published: 2013-07-31  

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