2011 Fiscal Year Final Research Report
THE DEVELOPMENT OF NEWLY JAPAN-ORIGINAL GENE THERAPY FOR DEGENERATIVE ARTHROSIS USING DECOY GENE.
| Project/Area Number |
21659433
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Shimane University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | 免疫 / 感染 / 炎症 |
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| Research Abstract |
Examination for the expression of uPA and MMPs stimulated by several cytokines using cultured cells. The expression of uPA and MMPs was increased by the stimulation with TNFαusing cultured cells derived from cartilaginous tissue of TMJ. Examination of transcriptional factor related to cytokines stimulation. Transcriptional factors, NF-kB and Sp1, were activated by TNFαstimulation at the cultured cells derived from cartilaginous tissue. Establishment of the condition for the gene transfer We established the condition for the Decoy oligodeoxynucletides targeted to NF-kB and Sp1 using HVJ-liposome method, and decoy ODNs were routinely transferred to 100% of the cells. Inhibition of the expression of uPA and MMPs by Decoy ODNs transfection The expression of the uPA and MMPs were inhibited by the transfection of Decoy ODNs to the cultured cells derived from cartilaginous tissue.
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