Human iPS cells from oral mucosal tissues using the liposome non-viral gene delivery system

2011 Fiscal Year Final Research Report

• Project/Area Number: 21659490
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Social dentistry
• Research Institution: Tsurumi University
• Principal Investigator: HANADA Nobuhiro 鶴見大学, 歯学部, 教授 (70180916)
• Co-Investigator(Kenkyū-buntansha): NOMURA Yoshiaki 鶴見大学, 歯学部, 准教授 (90350587) ; IMAI Susumu 鶴見大学, 歯学部, 講師 (80072958)
• Project Period (FY): 2009 – 2011
• Keywords: iPS細胞 / レトロウイルス / リポソーム / 歯科医療安全

Research Abstract

Traditional iPS cells establish methods used retrovirus transduction of the reprogramming 4 factors(Oct3/4, Sox2, Klf4, c-Myc) which results in viral integration into the genome. The use of retroviruses is potentially problematic for clinical applications involving cells derived from iPS cells due to the significant increased risk of inducing a cancer transformation. A non-viral gene delivery system should be required to establish iPS cells, for example lipsome methos.

Research Products

• [Journal Article] Human periodontal ligament fibroblasts are the optimal cell source for induced pluripotent stem cells (2012)
  • Author(s): Nomura Y, Ishikawa M, Yashiro Y, Sanggarnjanavanich S, Yamaguchi T, Arai C, Noda K, Takano Y, Nakamura Y, Hanada N
  • Journal Title: Histochem Cell Biol
• [Presentation] iPS細胞の安全性に関する検討第2報核型異常について (2012)
  • Author(s): 野村義明, 石川美佐緒, 新井千博, 八城祐一, 野田晃司, 山口貴央, 阿保備子, 角田衣理加, 山田秀則, 今井奨, 中村芳樹, 花田信弘
  • Organizer: 第61回日本口腔衛生学会総会
  • Place of Presentation: 横須賀市
  • Year and Date: 20120400
• [Presentation] ヒト歯根膜線維芽細胞からのiPS細胞の樹立 (2011)
  • Author(s): 石川美佐緒, 野村義明, 八城祐一, 新井千博, 山口貴央, 村田貴俊, 野田晃司, 石川雄一, 花田信弘, 中村芳樹
  • Organizer: 第53回歯科基礎医学会総会
  • Place of Presentation: 岐阜市
  • Year and Date: 20110900
• [Presentation] iPS細胞の安全性に関する検討 (2011)
  • Author(s): 野村義明, 石川美佐緒, 新井千博, 八城祐一, 野田晃司, 山口貴央, 阿保備子, 角田衣理加, 山田秀則, 今井奨, 中村芳樹, 花田信弘
  • Organizer: 第60回日本口腔衛生学会総会
  • Place of Presentation: 松戸市
  • Year and Date: 20110800