Development of next generation water resources circulation technology, using sulfur cycle microbes, applicable to developing countries

2013 Fiscal Year Final Research Report

• Project/Area Number: 21676004
• Research Category: Grant-in-Aid for Young Scientists (S)
• Allocation Type: Single-year Grants
• Research Field: Civil and environmental engineering
• Research Institution: Nagaoka University of Technology
• Principal Investigator: YAMAGUCHI Takashi 長岡技術科学大学, 工学研究科, 教授 (10280447)
• Research Collaborator: クーシビライ パイラヤ タイ王国、コンケン大学, 講師
• Project Period (FY): 2009 – 2013
• Keywords: 下水 / 微生物 / 生物処理 / 環境浄化 / 微生物解析 / 硫黄動態

Research Abstract

In order to fulfill the imperative need of proper organic matter removal technology for municipal wastewater treatment, an integrated system of Upflow Anaerobic Sludge Blanket and Down-flow Hanging Sponge reactor is discussed in this study. The findings of this study demonstrated that average Suspended Solid and Biochemical Oxygen Demand removal efficiency in summer (in winter) by the system were archived over 95% (over 85%). Therefore, this proposed system could be an innovative solution for present prevalent wastewater treatment problems. An extensive analysis of the diversity of microbes involved in anaerobic sulfur oxidation in the anaerobic reactor, it shown that bacteria belonging to the phylum Proteobacteria was observed as a major bacteria group.

Research Products

• [Journal Article] Molecular characterization of anaerobic sulfur-oxidizing microbial communities in up-flow anaerobic sludge blanket reactor treating municipal sewage (2014)
  • Author(s): A. A. Aida, M. Hatamoto, M.Yamamoto, S. Ono, A. Nakamura, M.Takahashi, T. Yamaguchi
  • Journal Title: Journal of Bioscience and Bioengineering
• [Journal Article] Microbial community composition of a down-flow hanging sponge (DHS) reactor combined with an up-flow anaerobic sludge blanket (UASB) reactor for the treatment of municipal sewage (2014)
  • Author(s): K. Kubota, M. Hayashi, K. Matsunaga, A. Iguchi, A. Ohashi, Y. Li, T. Yamaguchi, H. Harada
  • Journal Title: Bioresource Technology Pages: 144-150
• [Journal Article] Characterization of the retained sludge in a down-flow hanging sponge (DHS) reactor with emphasis on its low excess sludge production (2014)
  • Author(s): T. Onodera, K. Matsunaga, K. Kubota, R. Taniguchi, H. Harada, K. Syutsubo, T. Okubo, S. Uemura, N. Araki, M. Yamada, M. Yamauchi, T. Yamaguchi
  • Journal Title: Bioresource Technology Volume: 136 Pages: 169-175
• [Journal Article] Enhancement of denitrification in a down-flow hanging sponge reactor by effluent recirculation (2013)
  • Author(s): N. Ikeda, T. Natori, T. Okubo, A. Sugo, M. Aoki, M. Kimura, T. Yamaguchi, H. Harada, A. Ohashi, S. Uemura
  • Journal Title: Water Science and Technology Volume: 68(3) Pages: 591-598
• [Journal Article] Energy saving system with high effluent quality for municipal sewage treatment by UASB-DHS (2012)
  • Author(s): H. Tanaka, M. Takahashi, Y. Yoneyama, K. Syutsubo, K. Kato, A. Nagano, T. Yamaguchi, H. Harada
  • Journal Title: Water Science and Technology Volume: 66(6) Pages: 1186-1194
• [Journal Article] Detection of single-copy functional genes in prokaryotic cells by two-pass TSA-FISH with polynucleotide probes (2012)
  • Author(s): S. Kawakami, T. Hasegawa, H. Imachi, T. Yamaguchi, H. Harada, A. Ohashi, and K. Kubota
  • Journal Title: Journal of Microbiological Methods Volume: 88 Pages: 218-223
• [Journal Article] The effect of salinity on nitrite accumulation in a DHS reactor (2012)
  • Author(s): T. Natori, Y.Takemura, H. Harada, K. Abe, A. Ohashi, M. Kimura, T. Yamaguchi, T. Okubo, S. Uemura
  • Journal Title: Journal of Chemical Technology and Biotechnology Volume: 87 Pages: 1466-1472
• [Journal Article] Enrichment of anammox bacteria from mudflat sediments collected in Tokyo Bay (2012)
  • Author(s): S. Uemura, N. Ikeda, T. Natori, T. Okubo, T. Yamaguchi, M. Kimura, A. Iguchi, H. Harada
  • Journal Title: African Journal of Microbiology Research Volume: 6(16) Pages: 3778-3782
• [Journal Article] Long term evaluation of the effect of salinity on organic removal and ammonium oxidation in a down-flow hanging sponge reactor (2012)
  • Author(s): S. Uemura, M. Kimura, T. Yamaguchi, A. Ohashi, Y. Takemura, H. Harada
  • Journal Title: International Journal of Environmental Research Volume: 6(2), Spring Pages: 361-366
• [Journal Article] Methanolinea mesophila sp. nov., a hydrogenotrophic methanogen isolated from rice field soil, and proposal of the archaeal family Methanoregulaceae fam. nov. within the order Methanomicrobiales (2012)
  • Author(s): S. Sakai, M. Ehara, 1.C. Tseng, T. Yamaguchi, S. L. Brauer, H. Cadillo-Quiroz, S. H. Zinder, H. Imachi
  • Journal Title: International Journal of Environmental Research Volume: 62(6) Pages: 1389-1395
• [Journal Article] Direct treatment of settled sewage by DHS reactors with different size sponge support media (2012)
  • Author(s): S. Uemura, S. Suzuki, Y. Maruyama, A. Ohashi, T. Yamaguchi, H. Harada
  • Journal Title: International Journal of Environmental Research Volume: 6(1) Pages: 25-32
• [Journal Article] Cultivation of methanogenic community from subseafloor sediments using a continuous-flow bioreactor (2011)
  • Author(s): H. Imachi, K. Aoi, E. Tasumi, Y. Saito, Y. Yamanaka, Y. Saito, T. Yamaguchi, H. Tomaru, R. Takeuchi, Y. Morono, F. Inagaki, K. Takai
  • Journal Title: ISME J Volume: 5 Pages: 1913-1925
• [Journal Article] Methanoregula formicica sp. nov., a methane-producing archaeon isolated from methanogenic sludge (2011)
  • Author(s): Y. Tashiro, S. Sakai, M. Ehara, M. Miyazaki, T. Yamaguchi, H. Imachi
  • Journal Title: International Journal of Environmental Research Volume: 61(1) Pages: 53-59
• [Journal Article] Evaluation of sludge properties in a pilot scale UASB reactor for sewage treatment in temperate region (2011)
  • Author(s): K. Syutsubo, W. Yoochatchaval, 1. Tsushima, N. Araki, K. Kubota, T. Onodera, M. Takahashi, T. Yamaguchi, Y. Yoneyama
  • Journal Title: Water Science and Technology Volume: 64(10) Pages: 1959-1966
• [Journal Article] Evaluation of treatment characteristics and sludge properties in a UASB reactor treating municipal sewage at ambient temperature (2011)
  • Author(s): M. Takahashi, A. Ohya, S. Kawakami, Y. Yoneyama, T. Onodera, K. Syutsubo, S. Yamazaki, N. Araki, H. Harada, T. Yamaguchi
  • Journal Title: International Journal of Environmental Research Volume: 5(4) Pages: 821-826
• [Journal Article] Performance of a pilot-scale sewage treatment : An up-flow anaerobic sludge blanket (UASB) and a down-flow hanging sponge (DHS) reactors combined system by sulfur-redox reaction process under low-temperature conditions (2011)
  • Author(s): M. Takahashi, T. Yamaguchi, Y. Kuramoto, A. Nagano, S. Shimozaki, H. Sumino, N. Araki, S. Yamazaki, S. Kawakami, H. Harada
  • Journal Title: Bioresource Technology Volume: 102 Pages: 753-757
• [Journal Article] Removal of organic substances and oxidation of ammonium nitrogen by a down-flow hanging sponge (DHS) reactor under high salinity conditions (2010)
  • Author(s): S. Uemura, S. Suzuki, K. Abe, K. Kubota, T.Yamaguchi, A. Ohashi, Y. Takemura, H. Harada
  • Journal Title: Bioresource Technology Volume: 101 Pages: 5180-5185
• [Journal Article] Application of a Single-chamber Microbial Fuel Cell (MFC) for organic wastewater treatment : influence of changes in wastewater composition on the process performance (2010)
  • Author(s): K. Kubota, W. Yoochatchaval, T. Yamaguchi, K. Syutsubo
  • Journal Title: Sustainable Environment Research Volume: 6(20) Pages: 347-351
• [Journal Article] Evaluation of sludge properties in a pilot scale UASB reactor for sewage treatment in temperate region (2010)
  • Author(s): K. Syutsubo, W. Yoochatchaval, I. Tsushima, N. Araki, K. Kubota, T. Onodera, M. Takahashi, T. Yamaguchi, Y. Yoneyama
  • Journal Title: 12th World. Congr. Anaerobic Dig Volume: 90
• [Journal Article] Detection of denitrifying bacteria by tyramide signal amplification - fluorescence in situ hybridization (TSA-FISH) (2010)
  • Author(s): M. Aoki, K. Maetani, N. Araki, S. Kawakami, T. Yamaguchi
  • Journal Title: 17th Asian Symposium on Ecotechnology Volume: 26
• [Journal Article] Development of 16S rRNA gene-targeted primers for detection of archaeal anaerobic methanotrophs (ANMEs) (2009)
  • Author(s): A. Miyashita, H. Mochimaru, H. Kazawa, A. Ohashi, T. Yamaguchi, K. Horikoshi, T. Nunoura, K. Takai, H. Imachi
  • Journal Title: FEMS Microbiology. Letter Volume: 297(1) Pages: 31-37
• [Journal Article] In Situ Detection and Quantification of Uncultured Members of the Phylum Nitrospirae Abundant in Methanogenic Wastewater Treatment Systems (2009)
  • Author(s): A. Iguchi, T. Terada, T. Narihiro, T. Yamaguchi, Y. Kamagata, Y. Sekiguchi
  • Journal Title: Microbes and Environments Volume: 24(2) Pages: 97-104
• [Journal Article] Methanofollis ethanolicus sp. nov.,an ethanol-utilizing methanogen isolated from a lotus field (2009)
  • Author(s): H. Imachi, S. Sakai, H. Nagai, T. Yamaguchi, K. Takai
  • Journal Title: International Journal of Environmental Research Volume: 59 Pages: 800-805
• [Journal Article] Influence of sugar content of wastewater on the microbial characteristics of granular sludge developed at 20℃ in the anaerobic granular sludge bed reactor (2009)
  • Author(s): W. Yoochatchaval, I. Tsushima, T. Yamaguchi, N. Araki, H. Sumino, A. Ohashi, H. Harada, K. Syutsubo
  • Journal Title: Journal of Environmental Science and Health PART A (Toxic/Hazardous Substance Environmental Engineering) Volume: 44 Pages: 921-927
• [Journal Article] H. Tanaka, M. Takahashi, Y. Yoneyama, K. Syutsubo, K. Kato, A. Nagano, T.Yamaguchi, H. Harada
  • Author(s): Down-flow hanging sponge; energy saving;excess sludge;municipal sewage treatment; up-flow anaerobic sludge blanket
  • Journal Title: Water Science and Technology
  • DOI: doi:10.2166/wst.2012.297.
• [Presentation] Detection of Archaea in an upflow anaerobic sludge blanket reactor by in situ hybridization chain reaction-fluorescence in situ hybridization (2013)
  • Author(s): T. Yamaguchi, S. Kawakami, M. Hatamoto, M. Takahashi, K. Kubota, H. Imachi, N. Araki, T. Yamaguchi
  • Organizer: The 13th World Congress on Anaerobic Digestion
  • Year and Date: 20130000
• [Presentation] A novel detection method for visualizing environmental microbes with low rRNA content and low cell permeability by using in situ HCR-FISH (2013)
  • Author(s): T. Yamaguchi, S. Kawakami, M. Hatamoto, M. Takahashi, K. Kubota, H. Imachi, N. Araki, T. Yamaguchi
  • Organizer: Annual Conference of the Association for General and Applied Microbiology
  • Year and Date: 20130000
• [Presentation] Molecular analysis of microbial communities involved in anaerobic sulphur-oxidation in UASB reactor treating municipal sewage (2013)
  • Author(s): A. A. Azmi, M. Hatamoto, M. Yamamoto, S. Ono, M. Takahashi, T. Yamaguchi
  • Organizer: 13th World Congress on Anaerobic Digestion
  • Year and Date: 20130000
• [Presentation] Phylogenetic diversity analysis of relevant to anaerobic/anoxic wastewater treatment (2013)
  • Author(s): K. Kuroda, M. Hatamoto, A. Nakamura, M. Yamauchi, M. Yanlada, T. Yamaguchi
  • Organizer: The 3rd International Symposium on Technology for Sustainability, accepted
  • Year and Date: 20130000
• [Presentation] An integrated system of Sedimentation-DHS-A2SBR for effective removal of organic matter and nutrients from municipal wastewater (2013)
  • Author(s): N. Maharjan, K. Dehanla, K. Ohtsuki, Y. Saito, Y. Miyaoka, T. Tshering, A. Nakamura, M. Hatamoto, T. Yamaguchi
  • Organizer: Water and Environment Technology Conference
  • Year and Date: 20130000
• [Presentation] Assessment of combined system of UASB-DHS-A_2SBR for municipal sewage treatment (2013)
  • Author(s): Namita M., K. DEHAMA, K. OHTSUKI, T. CHOSEI, K. SAKAMOTO, Y. SAITO, Y. MIYAOKA, T.TSHERING, A. NAKAMURA, M. HATAMOTO and T.Yamaguchi
  • Organizer: The 3rd International Symposium on Technology for Sustainability
  • Year and Date: 20130000
• [Presentation] In situ HCR-FISH : a new sensitive fluorescence in situ hybridization technique for detecting environmental microorganisms (2012)
  • Author(s): T. Yamaguchi, S. Kawakami, M. Hatamoto, M. Takahashi, K. Kubota, H. Imachi, N. Araki, T. Yamaguchi
  • Organizer: 14th International Symposium on Microbial Ecology
  • Year and Date: 20120000
• [Presentation] Microbial communities involved in anaerobic sulfur-oxidation in UASB reactor treating municipal sewage (2012)
  • Author(s): A. A. Azmi, M. Hatamoto, M. Yamamoto, S. Ono, A. Nakamura, M. Takahashi, T. Yamaguchi
  • Organizer: The 4th IWA Asia-Pacific Young Water Professionals Conference
  • Year and Date: 20120000
• [Presentation] Characterization of anaerobic sulfur-oxidizing microbial communities in a UASB reactor treating municipal sewage (2012)
  • Author(s): A. A. Azmi, M. Hatamoto, S. Ono, A. Nakamura, M. Takahashi, T. Yamaguchi
  • Organizer: Water and Environnmnt Technology Conference
  • Year and Date: 20120000
• [Book] 総説 -嫌気性生物処理を中心に,排水・汚水処理技術集成vol.2、第2編第1章 処理技術研究の最前線の総説を執筆 (2013)
  • Author(s): 山口隆司,幡本将史,中村明靖,多川正,山崎慎一
• [Book] 微生物燃料電池による廃水処理システム最前線(第1編、第2章、第2節) (2013)
  • Author(s): 山口隆司、山口剛士、幡本将史、中村明靖、川上周司、久保田健吾
  • Publisher: NTS
• [Book] 水浄化技術の最新動向, 2章4節「次世代水資源循環技術」中のpp.121-131を担当) (2011)
  • Author(s): 山口隆司,高橋優信,幡本将史,川上周司,久保田健吾,原田秀樹,山田真義,山内正仁,荒木信夫, 山崎慎一
  • Publisher: シーエムシー出版
• [Remarks]
  • URL: http://ecolab.nagaokaut.ac.jp/