2011 Fiscal Year Final Research Report
Construction of biocatalysts based on substrate misrecognition of enzymes
| Project/Area Number |
21685018
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Nagoya University |
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Principal Investigator |
SHOJI Osami 名古屋大学, 理学研究科, 助教 (90379587)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 酵素化学 |
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| Research Abstract |
We have demonstrated that the substrate specificity of cytochrome P450s can be altered by inducing their substrate misrecognition using a decoy molecule that has a structural similarity to those of natural substrate. Accordingly, we have succeeded in constructing a novel reaction system that catalyzes oxidation reactions of non-natural substrates without any mutagenesis. Notably, our reaction system can alter the enantioselectivity of products just by changing the structure of the decoy molecules.
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