2011 Fiscal Year Final Research Report
Development of CLIP-RNAi method
| Project/Area Number |
21686078
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Okayama University |
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Principal Investigator |
OHTSUKI Takashi 岡山大学, 大学院・自然科学研究科, 教授 (80321735)
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| Project Period (FY) |
2009 – 2011
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| Keywords | RNAi / 光誘導 |
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| Research Abstract |
RNAi is currently an important experimental tool for basic research into gene function. RNAi also has many potential uses in various biotechnological and therapeutic applications. We recently developed photoinducible RNAi method, termed CLIP-RNAi. In this study, we improved the photoresponsive RNA carrier for CLIP-RNAi. We demonstrate the use of CLIP-RNAi with several fluorescent dyes that can be excited at different wavelengths. We used CLIP-RNAi method in in vitro and in vivo applications.
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