X-ray structural analysis of the vault with a molecular weight of 10 MDa.

2011 Fiscal Year Final Research Report

• Project/Area Number: 21687012
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Osaka University
• Principal Investigator: TANAKA Hideaki 大阪大学, 蛋白質研究所, 助教 (40346169)
• Project Period (FY): 2009 – 2011
• Keywords: ボルト / X線結晶構造解析 / 自然免疫 / 脂質ラフト

Research Abstract

I have obtained high quality crystals of the vault which diffracted x-rays at 2. 8Åresolution on beam line BL44XU at the SPring-8.

Research Products

• [Journal Article] ミニレビュー「全立体構造決定から切り開く謎の巨大粒子ボルトの機能解明」 (2011)
  • Author(s): 田中秀明、加藤公児、住澤知之、山下栄樹
  • Journal Title: 生化学 Volume: 83 Pages: 392-395
• [Journal Article] 分子量が1000万にも及ぶ生体内巨大分子に対する構造解析 (2009)
  • Author(s): 山下栄樹、加藤公児、田中秀明
  • Journal Title: 日本放射光学会誌 Volume: 22 Pages: 284-291
• [Journal Article] 最近の研究から「分子量約1000万の巨大粒子vaultのX線結晶構造」 (2009)
  • Author(s): 田中秀明、加藤公児
  • Journal Title: 日本結晶学会誌 Volume: 51 Pages: 189-194
• [Journal Article] Short Review「細胞内にある最大の核酸-蛋白質複合体vaultのX線結晶構造」 (2009)
  • Author(s): 加藤公児、田中秀明
  • Journal Title: 蛋白質核酸酵素 Volume: 54 Pages: 1159-1165
• [Journal Article] カレントトピックス「巨大粒子vaultの形から見えてきたこと」 (2009)
  • Author(s): 住澤知之、田中秀明
  • Journal Title: 実験医学 Volume: 27 Pages: 1751-1754
• [Journal Article] トピックス新進気鋭シリーズ「分子量約1000万の巨大粒子vaultの全体構造決定から機能解明に迫る」 (2009)
  • Author(s): 田中秀明
  • Journal Title: 生物物理 Volume: 49 Pages: 302-303
• [Journal Article] The Structure of Rat Liver Vault at 3. 5 Angstrom Resolution (2009)
  • Author(s): Tanaka H, Kato K, Yamashita E, Sumizawa T, Zhou Y, Yao M, Iwasaki K, Yoshimura M and Tsukihara T
  • Journal Title: ANNUAL REPORT OF OSAKA UNIVERSITY 2008-2009 Volume: 100 Selected Papers Pages: 67
• [Journal Article] The Structure of Rat Liver Vault at 3. 5 Angstrom Resolution (2009)
  • Author(s): Kato K, Tanaka H and Tsukihara T
  • Journal Title: SPring-8 Research Frontiers 2009 Pages: 28-29
• [Presentation] The mechanisms of self-assembly of the vault (2011)
  • Author(s): Tanaka H
  • Organizer: the largest cytoplasmic ribonucleo-protein complex. XXII Congress and General Assembly of the International Union of Crystallography(IUCr2011)
  • Place of Presentation: Madrid, Spain
  • Year and Date: 20110823-24
• [Presentation] Structure determination of rat vault, a large nucleoprotein complex at 3. 5A resolution (2011)
  • Author(s): Tanaka H
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県
  • Year and Date: 2011-09-17
• [Presentation] The self-assembly mechanism of the vault (2011)
  • Author(s): 藤田千鶴子、田中秀明、月原冨武
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: 大阪府
  • Year and Date: 2011-06-07
• [Presentation] X-ray crystal structure of rat vault, a large nucleoprotein complex at 3. 5A resolution (2010)
  • Author(s): Tanaka H
  • Organizer: International Symposium at Chungbuk National University
  • Place of Presentation: Chungbuk, Korea
  • Year and Date: 20101117-18
• [Presentation] 謎の巨大分子ボルトの全立体構造情報の利用~機能解明とDDSへの応用を目指して～ (2010)
  • Author(s): 田中秀明
  • Organizer: SENRI(Seminar for Enchaining of Research & Industry)の会
  • Place of Presentation: 大阪府
  • Year and Date: 2010-12-24
• [Presentation] 分子量約1000万の核酸ータンパク質複合体vaultのX線結晶構造解析 (2009)
  • Author(s): 田中秀明
  • Organizer: 日本結晶学会年会
  • Place of Presentation: 兵庫県
  • Year and Date: 2009-12-03
• [Presentation] The mechanisms of self-assembly of the vault : the largest cytoplasmic ribonucleoprotein complex
  • Author(s): Tanaka H
  • Organizer: Pacifichem 2010
  • Place of Presentation: Hawaii, USA
• [Remarks] ボルトのイメージ図、ムービーなど_
  • URL: http://www.protein.osaka-u.ac.jp/crystallography/mvp/