Functional Analysis of the Psyllid Bacteriome to Harbor Carsonella, Mutualistic Bacteria with an Extremely Reduced Genome

2012 Fiscal Year Final Research Report

• Project/Area Number: 21687020
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Evolutionary biology
• Research Institution: Toyohashi University of Technology (2011-2012); The Institute of Physical and Chemical Research (2009-2010)
• Principal Investigator: NAKABACHI Atsushi 豊橋技術科学大学, エレクトロニクス先端融合研究所, 准教授 (40332267)
• Project Period (FY): 2009 – 2012
• Keywords: キジラミ / 共生 / 菌細胞 / カルソネラ / プロフテラ / 極小ゲノム / トランスクリプトーム解析 / ゲノム解析

Research Abstract

Transcriptome analysis of the bacteriome of the two psyllid species, P. venustaand D. citrirevealed genes that apparently play pivotal roles to maintain either the psyllid-Carsonellasymbiosis or the psyllid-Carsonella-Profftellasymbiosis.RNAi analysis gave insight into the function of these genes. Whole genome analysis of Carsonella_DC and Profftellarevealed their reductive evolution, demonstrating that Profftellais an unprecedented type of defensive symbiont.

Research Products

• [Journal Article] Defensive bacteriome symbiont with a drastically reduced genome. (2013)
  • Author(s): Nakabachi A, UeokaR, OshimaK, TetaR, MangoniA, GurguiM, OldhamNJ, van Echten-DeckertG, OkamuraK, YamamotoK, InoueH, OhkumaM, HongohY, MiyagishimaSY, HattoriM, PielJ, FukatsuT
  • Journal Title: CurrBiol
  • Peer Reviewed
• [Journal Article] Genome size of Pachypsylla venusta(Hemiptera: Psyllidae), and the ploidy of its bacteriocyte, the symbiotic host cellthat harbors intracellular mutualistic bacteria with the smallest cellular genome (2010)
  • Author(s): Nakabachi A, Koshikawa S, Miura T, Miyagishima S
  • Journal Title: Bull Entomol Res Volume: 100(1) Pages: 27-33
  • Peer Reviewed
• [Journal Article] Genome sequence of the pea aphid Acyrthosiphon pisum (2010)
  • Author(s): The International Aphid Genomics Consortium
  • Journal Title: PLoS Biol Volume: 8(2)e1000313
  • Peer Reviewed
• [Journal Article] Bacterial genes in the aphid genome: Absence of functional gene transfer from Buchnerato its host (2010)
  • Author(s): Nikoh N, McCutcheon JP, Kudo T, Miyagishima S, Moran NA, Nakabachi A.
  • Journal Title: PLoS Genet Volume: 6(2):e1000827
  • Peer Reviewed
• [Journal Article] Expansion of genes encoding a novel type of dynamin in the genome of the pea aphid, Acyrthosiphon pisum (2010)
  • Author(s): Nakabachi A, Miyagishima S.
  • Journal Title: Insect Mol Biol Volume: 19(s2) Pages: 165-173
  • Peer Reviewed
• [Journal Article] Chitinase-like proteins encoded in the genome of the pea aphid, Acyrthosiphon pisum (2010)
  • Author(s): Nakabachi A, Shigenobu S, Miyagishima S.
  • Journal Title: Insect Mol Biol Volume: 19(s2) Pages: 175-185
  • Peer Reviewed
• [Journal Article] A full-length cDNA resource for the pea aphid, Acyrthosiphon pisum (2010)
  • Author(s): Shigenobu S,Richards S, Cree AG, Morioka M, Fukatsu T, Kudo T, Miyagishima S, Gibbs RA, Stern D, Nakabachi A
  • Journal Title: Insect Mol Biol Volume: 19(s2) Pages: 23-31
  • Peer Reviewed
• [Journal Article] A genomic analysis of transcytosis in the pea aphid, Acyrthosiphon pisum, a mechanism involved in virus transmission (2010)
  • Author(s): Tamborindeguy C,MonsionB, BraultV, HunnicuttL, Ju HJ, NakabachiA, Van FleetE.
  • Journal Title: Insect Mol Biol Volume: 19(s2) Pages: 259-272
  • Peer Reviewed
• [Journal Article] Immunity and other defenses in pea aphids, Acyrthosiphon pisum. (2010)
  • Author(s): GerardoNM, AltincicekB, AnselmeC, AtamianH, BarribeauSM, de VosM, Duncan EJ, EvansJD, GabaldonT, GhanimM, HeddiA, KaloshianI, LatorreA, MoyaA, NakabachiA, ParkerBJ, Perez-BrocalV, PignatelliM, RahbeY, RamseyJS, Spragg C, TamamesJ, TamaritD, TamborindeguyC, Vincent-Monegat C, VilcinskasA.
  • Journal Title: Genome Biol Volume: 11(2):R21
  • Peer Reviewed
• [Journal Article] Genomic evidence for complementary purine metabolism in the pea aphid, Acyrthosiphon pisum, and its symbiotic bacterium Buchnera aphidicola. (2010)
  • Author(s): RamseyJS, MacDonald SJ, Jander G, Nakabachi A, Thomas GH, Douglas AE
  • Journal Title: Insect Mol Biol Volume: 19(s2) Pages: 241-248
  • Peer Reviewed
• [Presentation] Functions and evolution of a dual bacterial symbiosis in a serious citrus pest (2012)
  • Author(s): Nakabachi A, UeokaR, OshimaK, TetaR, MangoniA, GurguiM, OldhamNJ, van Echten-DeckertG, OkamuraK, YamamotoK, InoueH, OhkumaM, HongohY, MiyagishimaSY, HattoriM, PielJ, FukatsuT.
  • Organizer: 第28回日本微生物生態学会大会
  • Place of Presentation: 豊橋技術科学大学(豊橋)
  • Year and Date: 2012-09-20
• [Presentation] Aphids acquiredsymbiotic genes from bacteria (2012)
  • Author(s): Nakabachi A
  • Organizer: XXIV International Congress of Entomology
  • Place of Presentation: Daegu, Korea
  • Year and Date: 2012-08-20
• [Presentation] ミカンキジラミの防衛共生 (2012)
  • Author(s): 中鉢淳、上岡麗子、大島健志朗、Alfonso Mangoni、Mihaela Gurgui、Neil Oldham、Gerhild van Echten-Deckert、井上広光、大熊盛也、本郷裕一、宮城島進也、服部正平、Jorn Piel、深津武馬
  • Organizer: 第56回日本応用動物昆虫学会大会
  • Place of Presentation: 近畿大学(奈良)
  • Year and Date: 2012-03-28
• [Presentation] ミカンキジラミ共生細菌2種のゲノム構造と機能的役割 (2012)
  • Author(s): 中鉢淳、大島健志朗、上岡麗子、Alfonso Mangoni、Mihaela Gurgui、Neil Oldham、Gerhild van Echten-Deckert、井上広光、大熊盛也、本郷裕一、宮城島進也、Jorn Piel、服部正平、深津武馬
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 立教大学(東京)
  • Year and Date: 2012-03-10
• [Presentation] The obligate symbiosis in aphids: apromising target for highly selective pest control (2011)
  • Author(s): Nakabachi A
  • Organizer: The Asia-Pacific Interdisciplinary Research Conference 2011
  • Place of Presentation: Toyohashi University of Technology (Toyohashi).
  • Year and Date: 2011-11-18
• [Presentation] ミカンキジラミ必須共生細菌2種のゲノム解析 (2011)
  • Author(s): 中鉢淳、大島健志朗、宮城島進也、服部正平、深津武馬
  • Organizer: 第5回日本ゲノム微生物学会年会
  • Place of Presentation: 東北学院大学(仙台)
  • Year and Date: 2011-03-14
• [Presentation] 宿主昆虫と必須共生細菌のゲノム進化 (2010)
  • Author(s): 中鉢淳
  • Organizer: 日本進化学会大会第12回大会
  • Place of Presentation: 東京工業大学(東京)
  • Year and Date: 2010-08-03
• [Remarks]
  • URL: http://www.eiiris.tut.ac.jp/nakabachi.html