Regulatory Mechanisms for the quality of wound angiogenesis

2012 Fiscal Year Final Research Report

• Project/Area Number: 21689047
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Plastic surgery
• Research Institution: Keio University
• Principal Investigator: KUBOTA Yoshiaki 慶應義塾大学, 医学部, 特任講師 (50348687)
• Project Period (FY): 2009 – 2012
• Keywords: 創傷治癒 / 血管 / マクロファージ / 形態学

Research Abstract

Recently it was recognized that vascular density (vascular “quantity”) does not necessarily correlate with oxygen delivery (vascular “quality”) to tissues. This study was performed to uncover the mechanism which determines the vascular quality. The group

Research Products

• [Journal Article] Pathological neoangiogenesis depends on oxidative stress regulation by ATM. (2012)
  • Author(s): Okuno Y, Nakamura-Ishizu A, Otsu K, SudaT and *Kubota Y.
  • Journal Title: Nat Med Volume: 18(8) Pages: 1208-1216
  • URL: http://www.keio.ac.jp/ja/press_release/2012/kr7a4300000artgk.html
  • Peer Reviewed
• [Journal Article] Tumor angiogenesis and anti-angiogenic therapy (2012)
  • Author(s): *Kubota Y.
  • Journal Title: Keio J Med. Volume: 61(2) Pages: 47-56
  • Peer Reviewed
• [Journal Article] The formation of an angiogenic astrocyte template is regulated by the neuroretina in a HIF-1-dependent manner. (2012)
  • Author(s): Nakamura-Ishizu A, Kurihara T, Okuno Y, Ozawa Y, Kishi K, Goda N, Tsubota K, Okano H, Suda T and *Kubota Y.
  • Journal Title: Dev Biol Volume: 363(1) Pages: 106-14
  • Peer Reviewed
• [Journal Article] Genome-wide identification of endothelial cell-enriched genes in the mouse embryo (2012)
  • Author(s): Takase H, Matsumoto K, Yamadera R, Kubota Y, Otsu A, Suzuki R, Ishitobi H, Mochizuki H, Kojima T, Takano S, UchidaK, Takahashi S, and *Ema M
  • Journal Title: Blood Volume: 120(4) Pages: 914-23
  • Peer Reviewed
• [Journal Article] Extracellular matrix protein Tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration. (2012)
  • Author(s): Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T and *Kubota Y.
  • Journal Title: Blood Volume: 119(23) Pages: 5429-5437
  • Peer Reviewed
• [Journal Article] Isolation and function of mouse tissue resident vascular precursors marked by myelin protein zero. (2011)
  • Author(s): *Kubota Y, Takubo K, Hirashima M, Nagoshi N, Kishi K, Okuno Y,Nakamura-Ishizu A, Sano K, Murakami M,Ema M, Omatsu Y, Takahashi S, Nagasawa T, Shibuya M, Okano H and *Suda T
  • Journal Title: J Exp Med Volume: 208(5) Pages: 949-60
  • URL: http://www.keio.ac.jp/ja/press_release/2011/kr7a4300000685ib-att/110511_2.pdf
  • Peer Reviewed
• [Journal Article] Bone marrow-derived cells serve as pro-angiogenic macrophages but notendothelial cells in wound healing. (2011)
  • Author(s): Okuno Y, Nakamura-Ishizu A, Kishi K, Suda T and *Kubota Y.
  • Journal Title: Blood Volume: 117(19) Pages: 5264-72
  • Peer Reviewed
• [Journal Article] Sphere formation restores and confers hair-inducing capacity in cultured mesenchymal cells. (2011)
  • Author(s): Shimizu R, Okabe K, Kubota Y, Nakamura-Ishizu A, Nakajima H and *Kishi K.
  • Journal Title: Exp Dermatol Volume: 20(8) Pages: 679-81
  • Peer Reviewed
• [Journal Article] Vascularity of nongynecological leiomyosarcoma depends on colony-stimulating factor 1 but not on vascular endothelial growth factor (2011)
  • Author(s): *Kubota Y and Suda T
  • Journal Title: Am J Pathol Volume: 179(4) Pages: 1591-3
  • Peer Reviewed
• [Journal Article] von Hippel-Lindau protein regulates transition from fetal to adult circulatory system in retina (2010)
  • Author(s): Kurihara T, Kubota Y (equally first author), Ozawa Y, Takubo K, Noda K, Simon MC, Johnson RS, Suematsu M, Tsubota K, Ishida S, Goda N, *Suda T and *Okano H.
  • Journal Title: Development Volume: 137 Pages: 1563-1571
  • Peer Reviewed
• [Journal Article] Endothelial protein C receptor-expressing hematopoietic stem cells reside in the perisinusoidal niche in fetal liver. (2010)
  • Author(s): *Iwasaki H, Arai F, Kubota Y, Dahl M and*Suda T.
  • Journal Title: Blood Volume: 116(4) Pages: 544-53
  • Peer Reviewed
• [Journal Article] Regulation of the HIF-1alpha Level Is Essential for Hematopoietic Stem Cells. (2010)
  • Author(s): *Takubo K, Goda N, Yamada W, Iriuchishima H, Ikeda E, Kubota Y, Shima H, Johnson RS, Hirao A, Suematsu M,*Suda T.
  • Journal Title: Cell Stem Cell Volume: 7(3) Pages: 391-402
  • Peer Reviewed
• [Journal Article] M-CSF inhibition selectively targets pathological angiogenesis andlymphangiogenesis. (2009)
  • Author(s): *Kubota Y, Takubo K, Shimizu T, Ohno H,Kishi K, Shibuya M, Saya H and *Suda T
  • Journal Title: J Exp Med Volume: 206 Pages: 1089-102
  • Peer Reviewed
• [Journal Article] Feedback mechanism between blood vessels and astrocytes in retinal vascular development. (2009)
  • Author(s): *Kubota Y and *Suda T.
  • Journal Title: Trends Cardiovasc Med Volume: 19 Pages: 38-43
  • Peer Reviewed
• [Journal Article] Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow. (2009)
  • Author(s): Morikawa S, Mabuchi Y, Kubota Y, Nagai Y, Niibe K, Hiratsu E, Suzuki S, Miyauchi-Hara C, Nagoshi N, Sunabori T, Shimmura S, Miyawaki A, Nakagawa T, Suda T, Okano H and *Matsuzaki Y
  • Journal Title: J Exp Med Volume: 206 Pages: 2483-96
  • Peer Reviewed
• [Presentation] Atm function in angiogenesis (2012)
  • Author(s): 久保田義顕
  • Organizer: 第35回日本分子生物学会年会 ワークショップ「ATMファミリーキナーゼの多様な制御機構と組織・動物種特異的な役割」
  • Place of Presentation: 福岡
  • Year and Date: 20121211-14
• [Presentation] Neuroretinal regulation of vascular patterning. (2012)
  • Author(s): Kubota Y
  • Organizer: Neuro-Vascular Wiring Symposium 2012
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 20121112-13
• [Presentation] 抗腫瘍血管新生療法の新規標的 (2012)
  • Author(s): 久保田義顕
  • Organizer: Beyond VEGF blockade 第30回日本骨代謝学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 20120719-21
• [Presentation] 腫瘍血管特異的分子メカニズムの解明 (2012)
  • Author(s): 久保田義顕
  • Organizer: 千葉大学G-COEセミナー
  • Place of Presentation: 千葉
  • Year and Date: 2012-12-17
• [Presentation] Oxidative stress in pathological angiogenesis. (2012)
  • Author(s): Kubota Y
  • Organizer: Special Seminar in Biomedicum Helsinki.
  • Place of Presentation: Biomedicum Helsinki, Helsinki, Finland
  • Year and Date: 2012-10-24
• [Presentation] Tumor angiogenesis and oxidative stress. (2012)
  • Author(s): Kubota Y.
  • Organizer: Joint JSPS-Karolinska Institute (KI) Symposium.
  • Place of Presentation: Karolinska Institute, Stockholm, Sweden
  • Year and Date: 2012-10-23
• [Presentation] マウス毛乳頭血管発生のメカニズム (2012)
  • Author(s): 久保田義顕
  • Organizer: 第2回細胞再生医療研究会
  • Place of Presentation: 神戸
  • Year and Date: 2012-07-29
• [Presentation] 血管パターニング制御機構と腫瘍血管新生. (2012)
  • Author(s): 久保田義顕
  • Organizer: 第39回NIH金曜会
  • Place of Presentation: Bethesda
  • Year and Date: 2012-06-01
• [Presentation] Pathological neoangiogenesis depends on oxidative stress regulation by ATM. (2012)
  • Author(s): Kubota Y.
  • Organizer: Mini Seminar in the Scripps Institute.
  • Place of Presentation: SanDiego, CA
  • Year and Date: 2012-03-12
• [Presentation] Pathological neoangiogenesis depends on oxidative stress regulation by AT M (2011)
  • Author(s): 久保田義顕
  • Organizer: 第34回日本分子生物学会年会ワークショップ「Dynamic linkage of vascular system and organ functions -novel roles of blood vessels in health and disease」
  • Place of Presentation: 横浜
  • Year and Date: 20111213-15
• [Presentation] Pathological neoangiogenesis depends on oxidative stress regulation by ATM. (2011)
  • Author(s): Kubota Y
  • Organizer: The Second Pacific Symposium on Vascular Biology.
  • Place of Presentation: Jeju, Korea
  • Year and Date: 20111030-1102
• [Presentation] ATM drives pathological angiogenesis by suppressing oxidative stress (2011)
  • Author(s): Kubota Y.
  • Organizer: 9th Japan-Korea vascular biology symposium.
  • Place of Presentation: Busan, Korea
  • Year and Date: 20110825-26
• [Presentation] Postnatal tissue-resident vascular precursor cells marked by myelin protein zero. (2011)
  • Author(s): 久保田義顕
  • Organizer: 新学術領域「血管-神経ワイヤリングにおける相互依存性の成立機構」班会議
  • Place of Presentation: 熊本
  • Year and Date: 20110818-20
• [Presentation] Role of Ataxia-telangiectasia mutated (ATM) in angiogenesis (2011)
  • Author(s): 久保田義顕
  • Organizer: 第7回Aso International Meeting
  • Place of Presentation: 熊本
  • Year and Date: 20110729-31
• [Presentation] ATM-mediated suppression of oxidative stress is required for pathological neovascular expansion. (2011)
  • Author(s): Kubota Y.
  • Organizer: 8th International Symposium on the Biology of Endothelial Cells.
  • Place of Presentation: 8th International Symposium on the Biology of Endothelial Cells.
  • Year and Date: 20110615-18
• [Presentation] マクロファージのリンパ管新生における役割 (2011)
  • Author(s): 久保田義顕
  • Organizer: 第35回日本リンパ学会総会
  • Place of Presentation: 東京
  • Year and Date: 20110603-05
• [Presentation] マクロファージを標的としたリンパ浮腫治療戦略 (2011)
  • Author(s): 久保田義顕、貴志和生
  • Organizer: 第54回日本形成外科学会総会・学術集会
  • Place of Presentation: 徳島
  • Year and Date: 20110413-15
• [Presentation] 抗腫瘍血管新生療法の新規標的の探索 (2011)
  • Author(s): 久保田義顕
  • Organizer: Beyond VEGF blockade第42回 ニューロ・オンコロジィの会
  • Place of Presentation: 東京
  • Year and Date: 2011-12-03
• [Presentation] 形成外科と免疫組織学的解析 (2011)
  • Author(s): 久保田義顕、貴志和生
  • Organizer: 第20回日本形成外科基礎学術集会
  • Place of Presentation: 東京
  • Year and Date: 2011-10-06
• [Presentation] 抗腫瘍血管新生療法の新規標的の探索 (2011)
  • Author(s): 久保田義顕
  • Organizer: 第80回北里記念式 北里賞受賞講演
  • Place of Presentation: 東京
  • Year and Date: 2011-06-10
• [Presentation] ATM-mediated suppression of oxidative stress is required for pathological neovascular expansion (2011)
  • Author(s): 久保田義顕
  • Organizer: 新学術領域「血管-神経ワイヤリングにおける相互依存性の成立機構」班会議
  • Place of Presentation: 神戸
  • Year and Date: 2011-01-18
• [Presentation] Role of macrophages in angiogenesis and lymphangiogenesis. (2010)
  • Author(s): 久保田義顕
  • Organizer: 第18回 日本血管生物医学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 20101201-03
• [Presentation] M-CSF as a therapeutic target for tumor lymph/angiogenesis. (2010)
  • Author(s): Kubota Y
  • Organizer: 5th Gordon Research Conference (Endothelial Cell Phenotypes In health & Disease).
  • Place of Presentation: Biddeford, ME
  • Year and Date: 20100808-13
• [Presentation] 組織血管前駆細胞(tissue-resident vascular precursors)の同定と機能解析 (2010)
  • Author(s): 久保田義顕
  • Organizer: 第11回 Organ Microcirculation Forum
  • Place of Presentation: 東京
  • Year and Date: 2010-11-04
• [Presentation] 網膜幹細胞の血管ネットワーク形成における役割 (2010)
  • Author(s): 久保田義顕
  • Organizer: 新学術領域「血管-神経ワイヤリングにおける相互依存性の成立機構」キックオフミーティング
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-13
• [Presentation] 血管・リンパ管新生におけるマクロファージの役割 (2010)
  • Author(s): 久保田義顕
  • Organizer: 第11回Pharmaco-Hematologyシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-06-19
• [Presentation] 血管・リンパ管新生におけるマクロファージの役割 (2009)
  • Author(s): 久保田義顕
  • Organizer: 第15回 血液科学セミナー
  • Place of Presentation: 東京
  • Year and Date: 20091121-22
• [Presentation] M-CSF inhibition selectively targets pathological angiogenesis and lymphangiogenesis. (2009)
  • Author(s): Kubota Y
  • Organizer: 5th International Conference on Tumor Microenvironment.
  • Place of Presentation: Versailles, France
  • Year and Date: 20091020-24
• [Presentation] 発生期・腫瘍リンパ管新生におけるマクロファージの役割 (2009)
  • Author(s): 久保田義顕
  • Organizer: 第1回リンパ学に関する松本カンファランス
  • Place of Presentation: 松本
  • Year and Date: 20090821-22
• [Remarks]
  • URL: http://www.careerpath-prj.keio.ac.jp/kanrinmaru/scholar/kubota/index.html