Clarification of mechanisms for hierarchical gene expression in RNA world and its application

2011 Fiscal Year Final Research Report

• Project/Area Number: 21689049
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Nagasaki University
• Principal Investigator: MORI Ryoichi 長崎大学, 大学院・医歯薬学総合研究科, 助教 (30509310)
• Project Period (FY): 2009 – 2011
• Keywords: 免疫 / 炎症 / microRNA / 次世代シークエンサー / 皮膚創傷治癒

Research Abstract

Inflammation-related miRNAs might be involved in regulation of inflammatory response and tissue fibrosis. However, these genes have not been identified yet. In the present study, we identified wound-related known and unknown miRNAs using next generation sequencing. Then we identified inflammation-related miRNAs and clarified its functions using histological, cellular and molecular biological, and imaging analysis in vivo and in vitro.

Research Products

• [Journal Article] Reporter bioassay systems for the phenotypic screening of candidate drugs (2011)
  • Author(s): Chiba T, Tsuchiya T, Mori R, Shimokawa I
  • Journal Title: Sensors Volume: 12 Pages: 1648-1656
  • Peer Reviewed
• [Journal Article] Release of TNF-αfrom macrophages is mediated by small GTPase Rab37 (2011)
  • Author(s): Mori R, Ikematsu K, Kitaguchi T, Kim SE, Okamoto M, Chiba T, Miyawaki A, Shimokawa I, Tsuboi T
  • Journal Title: European Journal of Immunology Volume: 41 Pages: 3230-3239
  • Peer Reviewed
• [Journal Article] Acute stress response modified by modest inhibition of growth hormone axis : a potential machinery of the anti-aging effect of calorie restriction (2011)
  • Author(s): Komatsu T, Trindade LS, Chiba T, Hayashi H, Henmi T, Ushiroda Y, Mori R, Shimokawa I
  • Journal Title: Mechanisms of Ageing and Development Volume: 132 Pages: 103-109
  • Peer Reviewed
• [Journal Article] Development of Calorie Restriction Mimetics as Therapeutics for Obesity, Diabetes, Inflammatory and Neurodegenerative Diseases (2010)
  • Author(s): Chiba T, Tsuchiya T, Komatsu T, Mori R, Hayashi H, Shimokawa I
  • Journal Title: Current Genomics Volume: 11 Pages: 562-567
  • Peer Reviewed
• [Journal Article] Wound-associated skin fibrosis : mechanisms and treatments based on modulating the inflammatory response. Endocrine (2010)
  • Author(s): Shaw TJ, Kishi K, Mori R
  • Journal Title: Metabolic & Immune Disorders-Drug Targets Volume: 10 Pages: 320-330
  • Peer Reviewed
• [Journal Article] Development of a bioassay to screen for chemicals mimicking the anti-aging effects of calorie restriction (2010)
  • Author(s): Chiba T, Tsuchiya T, Komatsu T, Mori R, Hayashi H, Shimano H, Spindler SR, Shimokawa I
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 401 Pages: 213-218
  • Peer Reviewed
• [Journal Article] FoxO1 is involved in the antineoplastic effect of calorie restriction (2010)
  • Author(s): Yamaza H, Komatsu T, Wakita S, Kijogi C, Park S, Hayashi H, Chiba T, Mori R, Furuyama T, Mori N, Shimokawa I
  • Journal Title: Aging Cell Volume: 9 Pages: 372-382
  • Peer Reviewed
• [Presentation] 皮膚創傷治癒過程における炎症反応特異的microRNAの同定 (2011)
  • Author(s): 森亮一、Kim Sang Eun、岡本百々子、千葉卓哉、下川功
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111213-16
• [Presentation] 皮膚創傷治癒過程における炎症反応特異的microRNAの同定 (2011)
  • Author(s): 森亮一
  • Organizer: 第3回日本RNAi研究会
  • Place of Presentation: 広島
  • Year and Date: 20110826-27
• [Presentation] マクロファージのサイトカイン分泌に関与する低分子量G蛋白質Rab familyの機能解析 (2011)
  • Author(s): 森亮一、間所俊介、Kim Sang Eun、小松利光、千葉卓哉、坪井貴司、下川功
  • Organizer: 第34回日本基礎老化学会
  • Place of Presentation: 東京
  • Year and Date: 20110617-18
• [Presentation] Molecular mechanisms for TNFαsecretion from macrophages (2011)
  • Author(s): Mori R
  • Organizer: Gordon Research Conferences-Tissue Repair & Regeneration
  • Place of Presentation: New Hampshire, USA
  • Year and Date: 20110605-10
• [Presentation] マクロファージにおけるtumor necrosis factor-αの分泌は低分子量G蛋白質Rab37によって制御される (2011)
  • Author(s): 森亮一、千葉卓哉、下川功
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20110428-30
• [Presentation] Identification of differentially expressed circulating microRNA-complexed with argonaute2 in blood plasma of calorie restriction and high fat diet-induced obese mice by next generation sequencing (2011)
  • Author(s): Mori R
  • Organizer: The 2nd Pusan-Nagasaki Joint Seminar on Aging Research
  • Place of Presentation: Pusan, Korea
  • Year and Date: 2011-01-16
• [Presentation] 次世代シーケンサーを用いたカロリー制限及び肥満マウス血漿中に存在するmicroRNAの網羅的同定 (2010)
  • Author(s): 森亮一、Kim San Eun、兼松宗太郎、千葉卓哉、小松利光、鈴木穣、下川功
  • Organizer: 第2回RNAi研究会
  • Place of Presentation: 広島
  • Year and Date: 20100827-28
• [Presentation] Identification of differentially expressed microRNA in blood plasma of calorie restricted and obese mice by next generation sequencing (2010)
  • Author(s): Mori R
  • Organizer: Asian Aging Core for Longevity Research and Education 2010
  • Place of Presentation: Jeju Island, Korea
  • Year and Date: 20100822-24
• [Presentation] 次世代シーケンサーを用いたカロリー制限及び肥満マウス血漿中に存在するmicroRNAの網羅的同定 (2010)
  • Author(s): 森亮一、Kim San Eun、兼松宗太郎、千葉卓哉、小松利光、鈴木穣、下川功
  • Organizer: 第33回日本基礎老化学会
  • Place of Presentation: 名古屋
  • Year and Date: 20100617-18
• [Presentation] デジタルトランスクリプトーム解析による炎症及び組織修復関連microRNAの網羅的同定 (2010)
  • Author(s): 森亮一、千葉卓哉、下川功
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 20100427-29
• [Presentation] Molecular mechanisms of cytokine secretion in macrophages (2009)
  • Author(s): 森亮一、池松和哉、下川功、坪井貴司
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-12