Roles of neuronal activity in circuit formation of the mammalian cerebral cortex

2010 Fiscal Year Final Research Report

• Project/Area Number: 21700350
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Kyoto University
• Principal Investigator: TAGAWA Yoshiaki Kyoto University, 大学院・理学研究科, 助教 (50303813)
• Project Period (FY): 2009 – 2010
• Keywords: 大脳皮質 / 神経回路形成 / 神経活動 / 発達 / 脳梁 / マウス / 電気穿孔法 / GFP

Research Abstract

This study aims to clarify the role of neuronal activity in circuit formation of the mammalian cerebral cortex. Levels and patterns of activity in mouse layer 2/3 cortical neurons during the perinatal period were manipulated in vivo, and their morphological development was assessed. Reducing activity in layer 2/3 neurons disturbed their long-range axonal projections. This defect was restored by increasing activity, suggesting that neuronal activity plays a pivotal role in their long-range axonal projections. In addition, repressing expression or function of an ion channel that is shown to be associated with an inherited mental retardation disturbed neuronal migration. Results suggest that control of neuronal activity is crucial for cortical circuit formation.

Research Products

• [Journal Article] Pre-synaptic and post-synaptic neuronal activity supports the axon development of callosal projection neurons during different post-natal periods in the mouse cerebral cortex. (2010)
  • Author(s): Mizuno H, Hirano T, Tagawa Y.
  • Journal Title: European Journal of Neuroscience 31 Pages: 410-424
  • Peer Reviewed
• [Journal Article] Control of cortical axon elongation by a GABA-driven Ca2+/calmodulin-dependent protein kinase cascade. (2009)
  • Author(s): Ageta-Ishihara N, Takemoto-Kimura S, Nonaka M, Adachi-Morishima A, Suzuki K, Kamijo S, Fujii H, Mano T, Blaeser F, Chatila TA, Mizuno H, Hirano T, Tagawa Y, Okuno H, Bito H.
  • Journal Title: Journal of Neuroscience 29(43) Pages: 13720-13729
  • Peer Reviewed
• [Presentation] Involvement of leak potassium channels in migration of cortical neurons. (2010)
  • Author(s): Bando Y, Hirano T, Tagawa Y.
  • Organizer: Soceity for Neuroscience annual meeting
  • Place of Presentation: San Diego、 USA
  • Year and Date: 2010-11-15
• [Presentation] A crucial role of leak potassium channels in neuronal migration. (2010)
  • Author(s): Bando Y, Hirano T, Tagawa Y.
  • Organizer: 日本神経科学大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-09-03
• [Presentation] A role of leak potassium channel KCNK9 in neuronal migration. (2009)
  • Author(s): Bando Y, Hirano T, Tagawa Y.
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Remarks] ホームページ等
  • URL: http://www.biophys.kyoto-u.ac.jp/hirano.php