2010 Fiscal Year Final Research Report
Analysis of neural tissue development using ES cells culture system
| Project/Area Number |
21700367
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neuroscience in general
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
EIRAKU Mototsugu The Institute of Physical and Chemical Research, 立体組織形成・解析ユニット, 副ユニットリーダー (40415097)
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| Project Period (FY) |
2009 – 2010
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| Keywords | ES細胞 / 形態形成 / 器官発生 / 試験管内再構築 |
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| Research Abstract |
In this study, we report the dynamic, autonomous formation of the optic cup (retinal primordium) structure from a three-dimensional culture of mouse ES cell aggregates. ES cell-derived retinal epithelium spontaneously formed hemispherical epithelial vesicles that become patterned along its proximal-distal axis. While the rigid proximal portion differentiated into pigment epithelium, the flexible distal portion progressively folded inward to form a shape reminiscent of the embryonic optic cup, exhibited interkinetic nuclear migration and generated stratified neural retinal tissue, just as seen in vivo. We demonstrate that optic-cup morphogenesis in this simple cell culture depends on an intrinsic self-organizing program involving stepwise and domain-specific regulations in local epithelial properties.
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Research Products
(5 results)
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[Journal Article] Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells.2010
Author(s)
Ohgushi M, Matsumura M, Eiraku M, Murakami K, Aramaki T, Nishiyama A, Muguruma K, Nakano T, Suga H, Ueno M, Ishizaki T, Suemori H, Narumiya S, Niwa H, Sasai Y
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Journal Title
Cell Stem Cell 7
Pages: 225-239
Peer Reviewed
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