The application of two photon excitation imaging to unanesthetised animal and the analysis of awakening brain function.

2010 Fiscal Year Final Research Report

• Project/Area Number: 21700372
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SOHYA Kazuhiro The Institute of Physical and Chemical Research, 大脳皮質回路可塑性研究チーム, 研究員 (80415207)
• Project Period (FY): 2009 – 2010
• Keywords: 二光子レーザー走査型顕微鏡 / カルシウムイメージング / 大脳皮質視覚野 / 方位(傾き)選択性 / in vivo / 光反応 / 蛍光タンパク質 / GABAergic Neuron(抑制性神経細胞)

Research Abstract

In this study, I applied in vivo two-photon functional calcium imaging of VGAT-Venus transgenic rats/mice, in which GABAergic neurons express Venus, a yellow fluorescent protein to the unanesthetized condition. And I analyzed response properties of excitatory and inhibitory neurons in the awakening brain function

Research Products

• [Journal Article] Laminar-specific maturation of GABAergic transmission and susceptibility to visual deprivation are related to endocannabinoid sensitivity in mouse visual cortex. (2010)
  • Author(s): Jiang B, Sohya K, Sarihi A, Yanagawa Y, Tsumoto T.
  • Journal Title: The Journal of Neuroscience 30 Pages: 14261-14272
  • Peer Reviewed
• [Journal Article] Ephrin-A5 and EphA5 interaction induces synaptogenesis during early hippocampal development. (2010)
  • Author(s): Akaneya Y, Sohya K, Kitamura A, Kimura F, Washburn C, Zhou R, Ninan I, Tsumoto T
  • Journal Title: Ziff EB.PLoS One 5 Pages: e12486(1-20)
  • Peer Reviewed
• [Journal Article] Difference in binocularity and ocular dominance plasticity between GABAergic and excitatory cortical neurons. (2010)
  • Author(s): Kameyama K, Sohya K (equal contribution), et al.
  • Journal Title: The Journal of Neuroscience 30 Pages: 1551-1559
  • Peer Reviewed
• [Presentation] Three-dimensional clusters of functional GA BAergic neurons in the mouse visualcortex. (2010)
  • Author(s): Ebina T, Sohya K, Yanagawa Y, Tsumoto T.
  • Organizer: 第33回日本神経科学大会(日本神経科学学会)
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-09-02
• [Presentation] Response properties of GABAergic and excitatory neurons in visual cortex of awake rats, revealed by two-photon functional calcium imaging. (2010)
  • Author(s): Kimura R, Sohya K, Ebina T, Isomura Y, Yanagawa Y, Cateau H, Tsumoto T.
  • Organizer: 第33回日本神経科学大会(日本神経科学学会)
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-09-02
• [Presentation] Differences in binocular responsiveness and ocular dominance plasticity between excitatory and GABAergic neurons in the mouse visual cortex. (2009)
  • Author(s): Sohya K, Kameyama K, Ebina T, Yanagawa Y, Tsumoto T.
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-09-17
• [Remarks] ホームページ等