Roles of Draxin signaling in formation of the corpus callosum and thalamocortical projections.

2010 Fiscal Year Final Research Report

• Project/Area Number: 21700382
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Kumamoto University
• Principal Investigator: SHINMYO Yohei Kumamoto University, 大学院・生命科学研究部, 助教 (00418831)
• Project Period (FY): 2009 – 2010
• Keywords: Draxin / 反発活性 / 脳梁 / 視床皮質神経

Research Abstract

In this study, we focused on Draxin functions in corpus callosum formation and thalamocortical projections. We found that Draxin has a critical role for the projections of corticothalamic and thalamocortical axons. Draxin is strongly expressed in early-born cortical neurons, including subplate and Cajal-Retzius cells, and weakly expressed in the ventral telencephalon and dorsal thalamus. Draxin deficient mice showed severe defects in the guidance of these axons : thalamocortical and corticothalamic axons followed an ectopic route through the external capsule instead of projecting to the cortex and thalamus, respectively. We found that thalamic axons showed a pathfinding error in the internal capsule region at embryonic day 14.5, when cortical axon pathfinding still appeared normal. This suggests that primary defects in the thalamocortical axon pathfinding cause misprojection of cortical axons. In addition, we showed that Draxin binds to thalamocortical axons and has inhibitory effects for neurite outgrowth from thalamic explants. Thus, we propose that Draxin repulsion from the neocortex may be essential for proper guidance of thalamocortical axons. We also found that Draxin repulsion is mediated by a receptor, dcc in the callosal neurons.

Research Products

• [Journal Article] Imaging of transgenic cricket embryos reveals cell movements consistent with a syncytial patterning mechanism. (2010)
  • Author(s): Nakamura T., Yoshizaki M., Ogawa S., Okamoto H., Shinmyo Y., Bando T., Ohuchi H., Noji S., Mito T.
  • Journal Title: Curr.Biol. 20 Pages: 1641-1647
• [Journal Article] Tsukushi is required for anterior commissure formation in mouse brain. (2010)
  • Author(s): Ito A., Shinmyo Y., Abe T., Oshima N., Tanaka H., Ohta K.
  • Journal Title: Biochem.Biophys.Res.Commun. 402 Pages: 813-818
  • Peer Reviewed
• [Journal Article] Olfactory bulb axonal outgrowth is inhibited by draxin. (2010)
  • Author(s): Ahmed G., Shinmyo Y., Naser I.B., Hossain M., Song X., Tanaka H.
  • Journal Title: Biochem.Biophys.Res.Commun. 398 Pages: 730-734
  • Peer Reviewed
• [Journal Article] Draxin is involved in the proper development of the dI3interneuron in chick spinal cord. (2010)
  • Author(s): Su Y., Zhang S., Islam S.M., Shinmyo Y., Naser I.B., Ahmed G., Tanaka H.
  • Journal Title: Dev.Dyn. 239 Pages: 1654-1663 2010
  • Peer Reviewed
• [Journal Article] PC3 is involved in the shift from proliferation to differentiation and maturation in spiral ganglion neurons. (2010)
  • Author(s): Hayashida M., Minoda R., Shinmyo Y., Ohta K., Tanaka H., Yumoto E.
  • Journal Title: Neuroreport 21 Pages: 90-93 2010
  • Peer Reviewed
• [Journal Article] Draxin, a repulsive axon guidance protein, is involved in hippocampal development. (2010)
  • Author(s): Zhang S., Su Y, Shinmyo Y., Islam S.M., Naser I.B., Ahmed G., Tamamaki N., Tanaka H.
  • Journal Title: Neurosci.Res. 66 Pages: 53-61
  • Peer Reviewed
• [Journal Article] Draxin, an axon guidance protein, affects chick trunk neural crest migration. (2009)
  • Author(s): Su Y., Naser I.B., Islam S.M., Zhang S., Ahmed G., Chen S., Shinmyo Y., Kawakami M., Yamamura K., Tanaka H.
  • Journal Title: Dev.Growth Differ. 51 Pages: 787-796
  • Peer Reviewed
• [Journal Article] Analysis of a repulsive axon guidance molecule, draxin, on ventrally directed axon projection in chick early embryonic midbrain. (2009)
  • Author(s): Naser I.B., Su Y., Islam S.M., Shinmyo Y., Zhang S., Ahmed G., Chen S., Tanaka H.
  • Journal Title: Dev.Biol. 332 Pages: 351-359
  • Peer Reviewed
• [Journal Article] 反撥性ガイダ ンス分子draxinは脊髄と大脳の交連線維形成に必須である (2009)
  • Author(s): 新明洋平、田中英明
  • Journal Title: 実験医学 Vol.27, No.9(6月号) Pages: 1376-1379
• [Journal Article] Draxin, a repulsive guidance protein for spinal cord and forebrain commissures. (2009)
  • Author(s): Islam S.M.Shinmyo Y., Okafuji T., Su Y., Naser I.B., Ahmed G., Zhang S., Chen S., Ohta K., Kiyonari H., Abe T., Tanaka S., Nishinakamura R., Terashima T., Kitamura T., Tanaka H.
  • Journal Title: Science These authors contributed equally to this work. 323 Pages: 388-393
  • Peer Reviewed
• [Journal Article] Ancestral functions of Delta/Notch signaling in the formation of body and leg segments in the cricket Gryllus bimaculatus.
  • Author(s): Mito T.Shinmyo Y.Kurita K., Nakamura T., Ohuchi H., Noji S.
  • Journal Title: Development in revision : These authors contributed equally to this work.
  • Peer Reviewed
• [Journal Article] Draxin Inhibits Axonal Outgrowth through the Netrin Receptor DCC.
  • Author(s): Ahmed G., Shinmyo Y., Ohta K., Islam S.M., Hossain M., Naser I.B., Riyadh A., Su Y., Zhang S., Tessier-Lavigne M., Tanaka H.
  • Journal Title: J.Neurosci in revision
  • Peer Reviewed
• [Presentation] draxin has a unique role for the guidance of thalamocortical projections (2011)
  • Author(s): Yohei Shinmyo, MD Riyadh, Iftekhar Bin Naser, Giasuddin Ahmed, Mahmud Hossain, Xiaohong Song, Kunimasa Ohta, Hideaki Tanaka.
  • Organizer: 日本発生生物学会44回大会
  • Place of Presentation: 沖縄 沖縄コンベンションセンター
  • Year and Date: 20110518-20110521
• [Presentation] draxin is essential for the guidance of thalamocortical projections (2010)
  • Author(s): Yohei Shinmyo, Tatsuya Okafuji, Iftekhar Bin Naser, Giasuddin Ahmed, Mahmud Hossain, Xiaohong Song, Kunimasa Ohta, Hideaki Tanaka
  • Organizer: 日本発生生物学会43回大会
  • Place of Presentation: 京都 国立京都国際会議
  • Year and Date: 20100620-20100623
• [Presentation] A novel repulsive guidance protein, Draxin. (2009)
  • Author(s): Yohei Shinmyo.
  • Organizer: Construction and Reconstruction of the Brain.
  • Place of Presentation: 淡路 淡路島夢舞台国際会議場
  • Year and Date: 20091008-20091010
• [Presentation] A novel repulsive guidance protein, draxin (2009)
  • Author(s): Yohei Shinmyo, Shahidul Islam, Tatsuya Okafuji, Yuhong Su, Iftekhar Naser, Giasuddin Ahmed, Sanbing Zhang, Kunimasa Ohta, Hideaki Tanaka.
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋 名古屋国際会議場
  • Year and Date: 20090916-20090918
• [Presentation] A novel repulsive guidance protein, Draxin (2009)
  • Author(s): Yohei Shinmyo, Shahidul Islam, Tatsuya Okafuji, Yuhong Su, Iftekhar Naser, Giasuddin Ahmed, Sanbing Zhang, Kunimasa Ohta, Hiroshi Kiyonari, Takaya Abe, Hideaki Tanaka
  • Organizer: 日本発生生物学会42回大会
  • Place of Presentation: 新潟 朱鷲メッセコンベンションセンター
  • Year and Date: 20090528-20090531