2010 Fiscal Year Final Research Report
Molecular characterization of a novel ER stress inducible factor, MANF
| Project/Area Number |
21700403
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Gifu University |
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Principal Investigator |
OH-HASHI Kentaro Gifu University, 工学部, 助教 (50332953)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 小胞体ストレス / ATF6 / MANF / CRELD2 |
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| Research Abstract |
Using microarray analysis and RT-PCR, we found that two types of MANF mRNA up-regulation by treatment with thapsigargin, an ER stress inducer. Afer constructing various expression plasmids of mouse MANF mutants in addition to two full-length mouse MANF, we evaluated the effects of MANF-overexpression on stress-induced cell death and examined the processing and secretion of wild type and mutated MANF.
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